Quantitative AgNORs study in ameloblastomas

Detalhes bibliográficos
Autor(a) principal: Santos, Anna Corrêa
Data de Publicação: 2008
Outros Autores: Tarquinio, Sandra Beatriz Chaves, Rivero, Elena Riet Corrêa, Araujo, Lenita Maria Áver, Krause, Cristina Iório
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/4266
Resumo: Purpose: Ameloblastomas are tumors that arise from the odontogenic epithelium, including the areas that coat the dentigerous cysts (DC). The aim of the present study was to investigate the proliferative activity present in luminal and mural areas of mural unicystic ameloblastomas (MUA), in conventional ameloblastomas (CA), and in DC, comparing them according to their biological behavior. Methods: AgNOR quantifications were performed using routine light microscopy under X100 magnification in 10 cases per type of lesion, considering 100 cells of representative areas of the tumor for each case. The AgNORs were observed as black dots within the cell nucleus. Results: MUA showed significant lower number of NORs/nucleus in the luminal proliferation area than in the area of mural proliferation and in CA (P<0.05). Conclusion: CA exhibit proliferative activity similar to the mural component of MUA, corroborating the pattern of higher aggressive clinical behavior of these tumor variants. Key words: Ameloblastoma; AgNOR; cell proliferation; dentigerous cyst
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spelling Quantitative AgNORs study in ameloblastomasAnálise quantitativa das AgNORs em ameloblastomasAmeloblastomaAgNORcell proliferationdentigerous cystOral BiologyOral PathologyPurpose: Ameloblastomas are tumors that arise from the odontogenic epithelium, including the areas that coat the dentigerous cysts (DC). The aim of the present study was to investigate the proliferative activity present in luminal and mural areas of mural unicystic ameloblastomas (MUA), in conventional ameloblastomas (CA), and in DC, comparing them according to their biological behavior. Methods: AgNOR quantifications were performed using routine light microscopy under X100 magnification in 10 cases per type of lesion, considering 100 cells of representative areas of the tumor for each case. The AgNORs were observed as black dots within the cell nucleus. Results: MUA showed significant lower number of NORs/nucleus in the luminal proliferation area than in the area of mural proliferation and in CA (P<0.05). Conclusion: CA exhibit proliferative activity similar to the mural component of MUA, corroborating the pattern of higher aggressive clinical behavior of these tumor variants. Key words: Ameloblastoma; AgNOR; cell proliferation; dentigerous cystObjetivo: Os ameloblastomas são tumores originados do epitélio odontogênico, inclusive daquele que reveste os cistos dentígeros (CD). O objetivo do presente trabalho foi avaliar a atividade proliferativa das áreas luminais e murais dos ameloblastomas unicísticos murais (AUM), ameloblastomas convencionais (AC) e cistos dentígeros (CD), comparando seus comportamentos biológicos. Metodologia: Para tanto foram selecionados 10 casos de AC, 10 casos de AUM e 10 amostras de CD. A análise quantificativa das AgNORs foi realizada através de microscopia ótica de rotina com aumento de 100X nos 10 casos de cada lesão estudada, analisando-se 100 células de áreas representativas do tumor. As AgNORs foram observadas como pontos pretos dentro do núcleo celular. Resultados: A média de NORs/núcleo da área de proliferação luminal do AUM foi estatisticamente inferior à da área de proliferação mural desta mesma lesão e à do AC (P<0,05); nenhuma outra diferença estatística foi encontrada. Conclusão: Conclui-se que os AC exibem atividade proliferativa semelhante à do componente mural do AUM, o que corrobora o padrão de comportamento clínico mais agressivo dessas variantes clínicas. Palavras-chave: Ameloblastoma; AgNORs; proliferação celular; cisto dentígeroEDIPUCRS - Editora Universitária da PUCRS2008-11-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articlelaboratoryapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/4266Revista Odonto Ciência; Vol. 24 No. 1 (2009); 9 - 14Revista Odonto Ciência; v. 24 n. 1 (2009); 9 - 141980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/4266/3660Santos, Anna CorrêaTarquinio, Sandra Beatriz ChavesRivero, Elena Riet CorrêaAraujo, Lenita Maria ÁverKrause, Cristina Iórioinfo:eu-repo/semantics/openAccess2013-11-11T13:23:39Zoai:ojs.revistaseletronicas.pucrs.br:article/4266Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-11T13:23:39Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Quantitative AgNORs study in ameloblastomas
Análise quantitativa das AgNORs em ameloblastomas
title Quantitative AgNORs study in ameloblastomas
spellingShingle Quantitative AgNORs study in ameloblastomas
Santos, Anna Corrêa
Ameloblastoma
AgNOR
cell proliferation
dentigerous cyst
Oral Biology
Oral Pathology
title_short Quantitative AgNORs study in ameloblastomas
title_full Quantitative AgNORs study in ameloblastomas
title_fullStr Quantitative AgNORs study in ameloblastomas
title_full_unstemmed Quantitative AgNORs study in ameloblastomas
title_sort Quantitative AgNORs study in ameloblastomas
author Santos, Anna Corrêa
author_facet Santos, Anna Corrêa
Tarquinio, Sandra Beatriz Chaves
Rivero, Elena Riet Corrêa
Araujo, Lenita Maria Áver
Krause, Cristina Iório
author_role author
author2 Tarquinio, Sandra Beatriz Chaves
Rivero, Elena Riet Corrêa
Araujo, Lenita Maria Áver
Krause, Cristina Iório
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Santos, Anna Corrêa
Tarquinio, Sandra Beatriz Chaves
Rivero, Elena Riet Corrêa
Araujo, Lenita Maria Áver
Krause, Cristina Iório
dc.subject.por.fl_str_mv Ameloblastoma
AgNOR
cell proliferation
dentigerous cyst
Oral Biology
Oral Pathology
topic Ameloblastoma
AgNOR
cell proliferation
dentigerous cyst
Oral Biology
Oral Pathology
description Purpose: Ameloblastomas are tumors that arise from the odontogenic epithelium, including the areas that coat the dentigerous cysts (DC). The aim of the present study was to investigate the proliferative activity present in luminal and mural areas of mural unicystic ameloblastomas (MUA), in conventional ameloblastomas (CA), and in DC, comparing them according to their biological behavior. Methods: AgNOR quantifications were performed using routine light microscopy under X100 magnification in 10 cases per type of lesion, considering 100 cells of representative areas of the tumor for each case. The AgNORs were observed as black dots within the cell nucleus. Results: MUA showed significant lower number of NORs/nucleus in the luminal proliferation area than in the area of mural proliferation and in CA (P<0.05). Conclusion: CA exhibit proliferative activity similar to the mural component of MUA, corroborating the pattern of higher aggressive clinical behavior of these tumor variants. Key words: Ameloblastoma; AgNOR; cell proliferation; dentigerous cyst
publishDate 2008
dc.date.none.fl_str_mv 2008-11-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
laboratory
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/4266
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/4266
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/4266/3660
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 24 No. 1 (2009); 9 - 14
Revista Odonto Ciência; v. 24 n. 1 (2009); 9 - 14
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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