Molecular characterization of Streptococcus mutans gtfB gene isolated from families

Detalhes bibliográficos
Autor(a) principal: Moreira, Monica
Data de Publicação: 2018
Outros Autores: Nascimento, Mariana Machado Fidelis do, Poletto, Mariane Moreira, Kliisiowicz, Débora do Rocio, Gomes, Renata Rodrigues, Vicente, Vânia Aparecida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/29727
Resumo: OBJECTIVE: Caries is a multifactorial infectious disease and the main etiological agent is the bacteria Streptococcus mutans due to its virulence factors, which enable the adherence to dental enamel and favours the formation of dental biofilm through the production of extracellular polysaccharides. The transmission of S. mutans can occur between people, often within families. The present study aimed to evaluate the intrafamily genetic pattern of S. mutans through partial sequencing of the gene that encodes the glucosyltransferase β (gtfB) enzyme.METHODS: We previously analyzed saliva samples from 40 individuals from nine families, and it was obtained 64 isolates biochemically characterized as S. mutans. The isolates were evaluated by random amplification of polymorphic DNA (RAPD). Those with greater similarity were characterized by partial sequence 16S rRNA gene and the gtfB gene sequencing.RESULTS: It was observed genetic similarity among strains isolated from individuals with caries activity; while isolates from individual without caries showed that they are genetically distinct, suggesting a different virulence pattern.CONCLUSION: The present results demonstrated that partial sequencing of the gtfB gene showed to be a potential marker to investigate genetic pattern and virulence of S. mutans, deserving further investigation in order to identify families at risk of caries.
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spelling Molecular characterization of Streptococcus mutans gtfB gene isolated from familiesMolecular characterization of Streptococcus mutans gtfB gene isolated from familiesStreptococcus mutansglucosyltransferase βvirulence.Streptococcus mutansglucosyltransferase βvirulence.OBJECTIVE: Caries is a multifactorial infectious disease and the main etiological agent is the bacteria Streptococcus mutans due to its virulence factors, which enable the adherence to dental enamel and favours the formation of dental biofilm through the production of extracellular polysaccharides. The transmission of S. mutans can occur between people, often within families. The present study aimed to evaluate the intrafamily genetic pattern of S. mutans through partial sequencing of the gene that encodes the glucosyltransferase β (gtfB) enzyme.METHODS: We previously analyzed saliva samples from 40 individuals from nine families, and it was obtained 64 isolates biochemically characterized as S. mutans. The isolates were evaluated by random amplification of polymorphic DNA (RAPD). Those with greater similarity were characterized by partial sequence 16S rRNA gene and the gtfB gene sequencing.RESULTS: It was observed genetic similarity among strains isolated from individuals with caries activity; while isolates from individual without caries showed that they are genetically distinct, suggesting a different virulence pattern.CONCLUSION: The present results demonstrated that partial sequencing of the gtfB gene showed to be a potential marker to investigate genetic pattern and virulence of S. mutans, deserving further investigation in order to identify families at risk of caries.OBJECTIVE: Caries is a multifactorial infectious disease and the main etiological agent is the bacteria Streptococcus mutans due to its virulence factors, which enable the adherence to dental enamel and favours the formation of dental biofilm through the production of extracellular polysaccharides. The transmission of S. mutans can occur between people, often within families. The present study aimed to evaluate the intrafamily genetic pattern of S. mutans through partial sequencing of the gene that encodes the glucosyltransferase β (gtfB) enzyme.METHODS: We previously analyzed saliva samples from 40 individuals from nine families, and it was obtained 64 isolates biochemically characterized as S. mutans. The isolates were evaluated by random amplification of polymorphic DNA (RAPD). Those with greater similarity were characterized by partial sequence 16S rRNA gene and the gtfB gene sequencing.RESULTS: It was observed genetic similarity among strains isolated from individuals with caries activity; while isolates from individual without caries showed that they are genetically distinct, suggesting a different virulence pattern.CONCLUSION: The present results demonstrated that partial sequencing of the gtfB gene showed to be a potential marker to investigate genetic pattern and virulence of S. mutans, deserving further investigation in order to identify families at risk of caries. *** Caracterização molecular do gene gtfB em Streptococcus mutans isolados de famílias ***OBJETIVO: A cárie é uma doença infecciosa multifatorial e o principal agente etiológico é a bactéria Streptococcus mutans devido a seus fatores de virulência, que permitem a adesão ao esmalte dental e favorecem a formação de biofilme dental através da produção de polissacarídeos extracelulares. A transmissão de S. mutans pode ocorrer entre as pessoas, muitas vezes na família. O presente estudo teve como objetivo avaliar o padrão genético intrafamiliar de S. mutans por meio de sequenciamento parcial do gene que codifica a enzima glicosiltransferase β (gtfB).METODOLOGIA: Foram previamente avaliadas amostras de saliva de 40 indivíduos de nove famílias, e obteve-se 64 isolados bioquimicamente caracterizados como S. mutans. Os isolados foram avaliados por amplificação aleatória de DNA polimórfico (RAPD). Aqueles com maior similaridade foram caracterizados pela sequência parcial do gene 16S rRNA e sequenciamento do gene gtfB.RESULTADOS: Observou-se similaridade genética entre as linhagens isoladas de indivíduos com atividade de cárie, enquanto isolados de indivíduo sem cárie mostraram-se geneticamente distintos, sugerindo-lhes um padrão de virulência diferente.CONCLUSÃO: Os resultados demonstraram que o sequenciamento parcial do gene gtfB mostrou ser um marcador potencial para investigar o padrão genético e a virulência de S. Mutans, merecendo mais investigação para identificar famílias em risco de cárie.Palavras-chave: Streptococcus mutans; glucosyltransferase β; virulência.EDIPUCRS - Editora Universitária da PUCRS2018-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2972710.15448/1980-6523.2018.1.29727Revista Odonto Ciência; Vol. 33 No. 1 (2018); 40-45Revista Odonto Ciência; v. 33 n. 1 (2018); 40-451980-65230102-946010.15448/1980-6523.2018.1reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/29727/17884Copyright (c) 2019 Revista Odonto Ciênciainfo:eu-repo/semantics/openAccessMoreira, MonicaNascimento, Mariana Machado Fidelis doPoletto, Mariane MoreiraKliisiowicz, Débora do RocioGomes, Renata RodriguesVicente, Vânia Aparecida2019-04-24T17:19:21Zoai:ojs.revistaseletronicas.pucrs.br:article/29727Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2019-04-24T17:19:21Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Molecular characterization of Streptococcus mutans gtfB gene isolated from families
Molecular characterization of Streptococcus mutans gtfB gene isolated from families
title Molecular characterization of Streptococcus mutans gtfB gene isolated from families
spellingShingle Molecular characterization of Streptococcus mutans gtfB gene isolated from families
Moreira, Monica
Streptococcus mutans
glucosyltransferase β
virulence.
Streptococcus mutans
glucosyltransferase β
virulence.
title_short Molecular characterization of Streptococcus mutans gtfB gene isolated from families
title_full Molecular characterization of Streptococcus mutans gtfB gene isolated from families
title_fullStr Molecular characterization of Streptococcus mutans gtfB gene isolated from families
title_full_unstemmed Molecular characterization of Streptococcus mutans gtfB gene isolated from families
title_sort Molecular characterization of Streptococcus mutans gtfB gene isolated from families
author Moreira, Monica
author_facet Moreira, Monica
Nascimento, Mariana Machado Fidelis do
Poletto, Mariane Moreira
Kliisiowicz, Débora do Rocio
Gomes, Renata Rodrigues
Vicente, Vânia Aparecida
author_role author
author2 Nascimento, Mariana Machado Fidelis do
Poletto, Mariane Moreira
Kliisiowicz, Débora do Rocio
Gomes, Renata Rodrigues
Vicente, Vânia Aparecida
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Moreira, Monica
Nascimento, Mariana Machado Fidelis do
Poletto, Mariane Moreira
Kliisiowicz, Débora do Rocio
Gomes, Renata Rodrigues
Vicente, Vânia Aparecida
dc.subject.por.fl_str_mv Streptococcus mutans
glucosyltransferase β
virulence.
Streptococcus mutans
glucosyltransferase β
virulence.
topic Streptococcus mutans
glucosyltransferase β
virulence.
Streptococcus mutans
glucosyltransferase β
virulence.
description OBJECTIVE: Caries is a multifactorial infectious disease and the main etiological agent is the bacteria Streptococcus mutans due to its virulence factors, which enable the adherence to dental enamel and favours the formation of dental biofilm through the production of extracellular polysaccharides. The transmission of S. mutans can occur between people, often within families. The present study aimed to evaluate the intrafamily genetic pattern of S. mutans through partial sequencing of the gene that encodes the glucosyltransferase β (gtfB) enzyme.METHODS: We previously analyzed saliva samples from 40 individuals from nine families, and it was obtained 64 isolates biochemically characterized as S. mutans. The isolates were evaluated by random amplification of polymorphic DNA (RAPD). Those with greater similarity were characterized by partial sequence 16S rRNA gene and the gtfB gene sequencing.RESULTS: It was observed genetic similarity among strains isolated from individuals with caries activity; while isolates from individual without caries showed that they are genetically distinct, suggesting a different virulence pattern.CONCLUSION: The present results demonstrated that partial sequencing of the gtfB gene showed to be a potential marker to investigate genetic pattern and virulence of S. mutans, deserving further investigation in order to identify families at risk of caries.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-30
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/29727
10.15448/1980-6523.2018.1.29727
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/29727
identifier_str_mv 10.15448/1980-6523.2018.1.29727
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/29727/17884
dc.rights.driver.fl_str_mv Copyright (c) 2019 Revista Odonto Ciência
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Revista Odonto Ciência
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 33 No. 1 (2018); 40-45
Revista Odonto Ciência; v. 33 n. 1 (2018); 40-45
1980-6523
0102-9460
10.15448/1980-6523.2018.1
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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