Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing

Detalhes bibliográficos
Autor(a) principal: de Almeida, Mailza Costa
Data de Publicação: 2017
Outros Autores: Ferreira, Luiz Carlos de Lima, Venâncio, Gisely Naura, de Souza, Risonilce Fernandes Silva, Lima, Emerson Silva, Conde, Nikeila Chacon de Oliveira, Bandeira, Maria Fulgência Costa Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/26479
Resumo: OBJECTIVE: The aim of this study was to histopathologically analyze, in subcutaneous connective tissue in rats, a propolis solution for cavity cleansing and its toxicity through hemolytic and Artemia franciscana tests.METHODS: Fifteen male rats were selected and randomly distributed in three experimental periods (07, 30 and 45 days), in which each animal received the four treatment groups in rounds: Group I – Propolis I; Group II – Propolis II; Group III – Calcium Hydroxide Water and Group IV – 2% Chlorexidine; the sides of the tube were the control group. The data were analyzed using descriptive statistics. The results showed, in terms of biocompatibility, that all materials presented a significant reduction of the inflammatory infiltrate and an increase of the thickness in the collagen fibers. It may be suggested, in decreasing order of biocompatibility, the use of following materials: calcium hydroxide-water, 2% chlorexidine, propolis I and propolis II.RESULTS: In the cytotoxicity test using A. franciscana, the propolis extract showed high toxicity when tested at concentrations and in the hemolytic activity test the propolis I extract showed greater activity than propolis II.CONCLUSION: The present study suggests the use of propolis as a cavity cleansing solution for shallow and medium cavities similar to 2% chlorexidine.
id PUC_RS-22_e0fc098d020402713ba1cd5cc072f874
oai_identifier_str oai:ojs.revistaseletronicas.pucrs.br:article/26479
network_acronym_str PUC_RS-22
network_name_str Revista odonto ciência (Online)
repository_id_str
spelling Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansingBiocompatibilidade da própolis no tecido subcutâneo de ratos: um possível biomaterial de limpeza de cavidadeMaterials testingPropolisDental cavity liningDetergentsNatural ProductsTeste de materiaisPrópolisForramento da cavidade dentáriaDetergentesOBJECTIVE: The aim of this study was to histopathologically analyze, in subcutaneous connective tissue in rats, a propolis solution for cavity cleansing and its toxicity through hemolytic and Artemia franciscana tests.METHODS: Fifteen male rats were selected and randomly distributed in three experimental periods (07, 30 and 45 days), in which each animal received the four treatment groups in rounds: Group I – Propolis I; Group II – Propolis II; Group III – Calcium Hydroxide Water and Group IV – 2% Chlorexidine; the sides of the tube were the control group. The data were analyzed using descriptive statistics. The results showed, in terms of biocompatibility, that all materials presented a significant reduction of the inflammatory infiltrate and an increase of the thickness in the collagen fibers. It may be suggested, in decreasing order of biocompatibility, the use of following materials: calcium hydroxide-water, 2% chlorexidine, propolis I and propolis II.RESULTS: In the cytotoxicity test using A. franciscana, the propolis extract showed high toxicity when tested at concentrations and in the hemolytic activity test the propolis I extract showed greater activity than propolis II.CONCLUSION: The present study suggests the use of propolis as a cavity cleansing solution for shallow and medium cavities similar to 2% chlorexidine.OBJETIVO: O objetivo deste estudo foi analisar histopatologicamente, em tecido conjuntivo subcutâneo de ratos, uma solução de própolis para a limpeza de cavidades e sua toxicidade através dos testes de hemólise e Artemia franciscana.METODOLOGIA: Foram utilizados 15 ratos machos, selecionados e distribuídos aleatoriamente em três grupos (n=5) em períodos experimentais (7, 30 e 45 dias), em que cada animal recebeu os quatro grupos de tratamento em forma de rodízio: Grupo I – Própolis I; Grupo II – Própolis II; Grupo III – Água de Hidróxido de Cálcio e Grupo IV – Clorexidina a 2%; as laterais do tubo foram o grupo controle. Os dados foram analisados pela estatística descritiva.RESULTADOS: Todos os materiais apresentaram uma redução significativa do infiltrado inflamatório e aumento da espessura das fibras colágenas. No teste de citotoxicidade de Artemia franciscana, o extrato de própolis apresentou alta toxicidade e no teste de atividade hemolítica o extrato de Própolis I mostrou-se mais ativo que o da Própolis II.CONCLUSÃO: O presente estudo mostrou a biocompatibilidade da própolis, sugerindo seu uso como solução de limpeza em cavidade cavidades rasas e médias semelhante à clorexidina a 2%.EDIPUCRS - Editora Universitária da PUCRS2017-11-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/2647910.15448/1980-6523.2017.2.26479Revista Odonto Ciência; Vol. 32 No. 2 (2017); 60-65Revista Odonto Ciência; v. 32 n. 2 (2017); 60-651980-65230102-946010.15448/1980-6523.2017.2reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/26479/16165Copyright (c) 2017 Revista Odonto Ciênciainfo:eu-repo/semantics/openAccessde Almeida, Mailza CostaFerreira, Luiz Carlos de LimaVenâncio, Gisely Naurade Souza, Risonilce Fernandes SilvaLima, Emerson SilvaConde, Nikeila Chacon de OliveiraBandeira, Maria Fulgência Costa Lima2018-04-04T16:33:36Zoai:ojs.revistaseletronicas.pucrs.br:article/26479Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2018-04-04T16:33:36Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing
Biocompatibilidade da própolis no tecido subcutâneo de ratos: um possível biomaterial de limpeza de cavidade
title Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing
spellingShingle Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing
de Almeida, Mailza Costa
Materials testing
Propolis
Dental cavity lining
Detergents
Natural Products
Teste de materiais
Própolis
Forramento da cavidade dentária
Detergentes
title_short Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing
title_full Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing
title_fullStr Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing
title_full_unstemmed Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing
title_sort Biocompatibility of propolis in subcutaneous tissue of rats: a possible biomaterial for cavity cleansing
author de Almeida, Mailza Costa
author_facet de Almeida, Mailza Costa
Ferreira, Luiz Carlos de Lima
Venâncio, Gisely Naura
de Souza, Risonilce Fernandes Silva
Lima, Emerson Silva
Conde, Nikeila Chacon de Oliveira
Bandeira, Maria Fulgência Costa Lima
author_role author
author2 Ferreira, Luiz Carlos de Lima
Venâncio, Gisely Naura
de Souza, Risonilce Fernandes Silva
Lima, Emerson Silva
Conde, Nikeila Chacon de Oliveira
Bandeira, Maria Fulgência Costa Lima
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv de Almeida, Mailza Costa
Ferreira, Luiz Carlos de Lima
Venâncio, Gisely Naura
de Souza, Risonilce Fernandes Silva
Lima, Emerson Silva
Conde, Nikeila Chacon de Oliveira
Bandeira, Maria Fulgência Costa Lima
dc.subject.por.fl_str_mv Materials testing
Propolis
Dental cavity lining
Detergents
Natural Products
Teste de materiais
Própolis
Forramento da cavidade dentária
Detergentes
topic Materials testing
Propolis
Dental cavity lining
Detergents
Natural Products
Teste de materiais
Própolis
Forramento da cavidade dentária
Detergentes
description OBJECTIVE: The aim of this study was to histopathologically analyze, in subcutaneous connective tissue in rats, a propolis solution for cavity cleansing and its toxicity through hemolytic and Artemia franciscana tests.METHODS: Fifteen male rats were selected and randomly distributed in three experimental periods (07, 30 and 45 days), in which each animal received the four treatment groups in rounds: Group I – Propolis I; Group II – Propolis II; Group III – Calcium Hydroxide Water and Group IV – 2% Chlorexidine; the sides of the tube were the control group. The data were analyzed using descriptive statistics. The results showed, in terms of biocompatibility, that all materials presented a significant reduction of the inflammatory infiltrate and an increase of the thickness in the collagen fibers. It may be suggested, in decreasing order of biocompatibility, the use of following materials: calcium hydroxide-water, 2% chlorexidine, propolis I and propolis II.RESULTS: In the cytotoxicity test using A. franciscana, the propolis extract showed high toxicity when tested at concentrations and in the hemolytic activity test the propolis I extract showed greater activity than propolis II.CONCLUSION: The present study suggests the use of propolis as a cavity cleansing solution for shallow and medium cavities similar to 2% chlorexidine.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/26479
10.15448/1980-6523.2017.2.26479
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/26479
identifier_str_mv 10.15448/1980-6523.2017.2.26479
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/26479/16165
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista Odonto Ciência
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista Odonto Ciência
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 32 No. 2 (2017); 60-65
Revista Odonto Ciência; v. 32 n. 2 (2017); 60-65
1980-6523
0102-9460
10.15448/1980-6523.2017.2
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
_version_ 1754820877020561408

Registros relacionados