Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista odonto ciência (Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3748 |
Resumo: | Purpose: The aim of the present work is to evaluate the behavior (survival and proliferation) of human third molar pulp cells cultivated in the presence or absence of amphotericin B. Methods: The cells were obtained from six impacted third molars extracted for orthodontic reasons. The pulp was minced into pieces, which were incubated at 37ºC for 60 minutes in buffer containing 0.2% collagenase. The cell suspension was divided into two tubes, centrifuged, and resuspensed in DMEM medium, supplemented with 10% fetal bovine serum, 100 units/mL penicillin, 100μg/mL streptomycin, 0.45μg/mL gentamincin, and 3.7mg/L HEPES with or without 2.5μg/mL of amphotericin B. The cells were plated in plastic culture dishes and incubated with 5% CO2/95% air at 37ºC. Results: Two out of the six tooth samples did not growth at any of the tested media. The other four samples growth in both media. The growth was more effective in the medium without amphotericin B. Conclusions: Although amphotericin B may prevent fungal contamination, it was concluded that amphotericin B may difficult the culture of human pulp cells. Key words: Amphotericin B; cell culture; dental pulp |
id |
PUC_RS-22_f07bdd6ea6f0dd8b0b90e57470425804 |
---|---|
oai_identifier_str |
oai:ojs.revistaseletronicas.pucrs.br:article/3748 |
network_acronym_str |
PUC_RS-22 |
network_name_str |
Revista odonto ciência (Online) |
repository_id_str |
|
spelling |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cellsUso de anfotericina B como antifúngico no meio de cultura para células pulpares humanasAmphotericin Bcell culturedental pulpDental pulpPurpose: The aim of the present work is to evaluate the behavior (survival and proliferation) of human third molar pulp cells cultivated in the presence or absence of amphotericin B. Methods: The cells were obtained from six impacted third molars extracted for orthodontic reasons. The pulp was minced into pieces, which were incubated at 37ºC for 60 minutes in buffer containing 0.2% collagenase. The cell suspension was divided into two tubes, centrifuged, and resuspensed in DMEM medium, supplemented with 10% fetal bovine serum, 100 units/mL penicillin, 100μg/mL streptomycin, 0.45μg/mL gentamincin, and 3.7mg/L HEPES with or without 2.5μg/mL of amphotericin B. The cells were plated in plastic culture dishes and incubated with 5% CO2/95% air at 37ºC. Results: Two out of the six tooth samples did not growth at any of the tested media. The other four samples growth in both media. The growth was more effective in the medium without amphotericin B. Conclusions: Although amphotericin B may prevent fungal contamination, it was concluded that amphotericin B may difficult the culture of human pulp cells. Key words: Amphotericin B; cell culture; dental pulpObjetivo: O presente trabalho tem como objetivo avaliar o comportamento de células da polpa, de terceiros molares humanos, cultivadas na presença ou ausência de um antifúngico (anfotericina B). Metodologia: As células pulpares foram obtidas de seis terceiros molares inclusos, após extração por motivos ortodônticos. A polpa foi cortada em fragmentos e estes foram incubados a 37ºC, por 60 minutos, em solução tampão contendo 0,2% de colagenase. A suspensão de células foi dividida em 2 tubos e centrifugada, sendo um pellet ressuspenso em meio – DMEM suplementado com 10% de soro fetal bovino, 100 unidades/mL de penicilina, 100μg/mL de estreptomicina, 0,45μg/mL de gentamicina e 3,7mg/L HEPES, e o outro ressuspenso no mesmo meio, porém contendo 2,5μg/mL de anfotericina B. As células foram semeadas em placas de cultura plásticas e incubadas em atmosfera 5% de CO2/95% de ar a 37ºC. Resultados: Dos seis dentes testados, dois não apresentaram crescimento celular em qualquer dos meios testados. Nos demais quatro dentes, houve crescimento celular em ambos meios, sendo este mais proeminente no meio sem anfotericina B. Conclusão: Apesar da anfotericina B ser importante para a prevenção de contaminações por fungos, concluiu-se que esta dificulta o estabelecimento de células pulpares humanas em cultura. Palavras-chave: Anfotericina B; cultura de células; polpa dentáriaEDIPUCRS - Editora Universitária da PUCRS2008-07-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articlelaboratory; cell culture; descriptiveapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3748Revista Odonto Ciência; Vol. 23 No. 1 (2008); 58 - 62Revista Odonto Ciência; v. 23 n. 1 (2008); 58 - 621980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3748/2874Luisi, Simone BonatoBarbachan, João Jorge DinizChies, José Artur BogoSant’ana Filho, Manoelinfo:eu-repo/semantics/openAccess2013-11-18T10:55:40Zoai:ojs.revistaseletronicas.pucrs.br:article/3748Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-18T10:55:40Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells Uso de anfotericina B como antifúngico no meio de cultura para células pulpares humanas |
title |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells |
spellingShingle |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells Luisi, Simone Bonato Amphotericin B cell culture dental pulp Dental pulp |
title_short |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells |
title_full |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells |
title_fullStr |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells |
title_full_unstemmed |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells |
title_sort |
Use of amphotericin B as antifungal agent in a culture medium for human dental pulp cells |
author |
Luisi, Simone Bonato |
author_facet |
Luisi, Simone Bonato Barbachan, João Jorge Diniz Chies, José Artur Bogo Sant’ana Filho, Manoel |
author_role |
author |
author2 |
Barbachan, João Jorge Diniz Chies, José Artur Bogo Sant’ana Filho, Manoel |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Luisi, Simone Bonato Barbachan, João Jorge Diniz Chies, José Artur Bogo Sant’ana Filho, Manoel |
dc.subject.por.fl_str_mv |
Amphotericin B cell culture dental pulp Dental pulp |
topic |
Amphotericin B cell culture dental pulp Dental pulp |
description |
Purpose: The aim of the present work is to evaluate the behavior (survival and proliferation) of human third molar pulp cells cultivated in the presence or absence of amphotericin B. Methods: The cells were obtained from six impacted third molars extracted for orthodontic reasons. The pulp was minced into pieces, which were incubated at 37ºC for 60 minutes in buffer containing 0.2% collagenase. The cell suspension was divided into two tubes, centrifuged, and resuspensed in DMEM medium, supplemented with 10% fetal bovine serum, 100 units/mL penicillin, 100μg/mL streptomycin, 0.45μg/mL gentamincin, and 3.7mg/L HEPES with or without 2.5μg/mL of amphotericin B. The cells were plated in plastic culture dishes and incubated with 5% CO2/95% air at 37ºC. Results: Two out of the six tooth samples did not growth at any of the tested media. The other four samples growth in both media. The growth was more effective in the medium without amphotericin B. Conclusions: Although amphotericin B may prevent fungal contamination, it was concluded that amphotericin B may difficult the culture of human pulp cells. Key words: Amphotericin B; cell culture; dental pulp |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-07-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article laboratory; cell culture; descriptive |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3748 |
url |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3748 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/3748/2874 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
publisher.none.fl_str_mv |
EDIPUCRS - Editora Universitária da PUCRS |
dc.source.none.fl_str_mv |
Revista Odonto Ciência; Vol. 23 No. 1 (2008); 58 - 62 Revista Odonto Ciência; v. 23 n. 1 (2008); 58 - 62 1980-6523 0102-9460 reponame:Revista odonto ciência (Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Revista odonto ciência (Online) |
collection |
Revista odonto ciência (Online) |
repository.name.fl_str_mv |
Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
||odontociencia@pucrs.br |
_version_ |
1754820874332012544 |