In vitro study of dose-response relationship of fluoride with dental enamel

Detalhes bibliográficos
Autor(a) principal: Arthur, Rodrigo Alex
Data de Publicação: 2007
Outros Autores: Giancristófaro, Magda, Tabchoury, Cínthia Pereira Machado, Cury, Jaime Aparecido
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista odonto ciência (Online)
Texto Completo: https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/1218
Resumo: In vitro models for evaluation of fluoride (F) reactivity should present dose-response effect. Thus, the aim of this study was to evaluate the fluoride dose-response present in aqueous solution with bovine dental enamel. A hundred and twenty bovine enamel slabs (5 × 5 × 2 mm), 60 sound and 60 with caries-like lesions, were subjected during 10 min to distilled and deionized water (negative control) and aqueous solutions containing 50, 100, 200 or 400 μg F/mL. Each experimental group received 12 sound and 12 carious slabs. Two consecutive layers of dental enamel were removed from all slabs, by acid etching, and fluoride extracted was determined with specific electrode. The results of fluoride incorporated were expressed in μg per g of removed enamel, considering the total amount of the two layers. Fluoride uptake by sound enamel showed a linear dose-response (p = 0.0001), while that by carious slabs showed a polynomial one (p < 0.0001). The results suggest that the in vitro model of reactivity used in the present study is appropriate to evaluate dose-response between fluoride present in aqueous solution and that incorporated by either sound or carious bovine dental enamel. UNITERMS: fluoride; dental enamel; reactivity; dose-response; in vitro study.
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spelling In vitro study of dose-response relationship of fluoride with dental enamelIn vitro study of dose-response relationship of fluoride with dental enamelIn vitro models for evaluation of fluoride (F) reactivity should present dose-response effect. Thus, the aim of this study was to evaluate the fluoride dose-response present in aqueous solution with bovine dental enamel. A hundred and twenty bovine enamel slabs (5 × 5 × 2 mm), 60 sound and 60 with caries-like lesions, were subjected during 10 min to distilled and deionized water (negative control) and aqueous solutions containing 50, 100, 200 or 400 μg F/mL. Each experimental group received 12 sound and 12 carious slabs. Two consecutive layers of dental enamel were removed from all slabs, by acid etching, and fluoride extracted was determined with specific electrode. The results of fluoride incorporated were expressed in μg per g of removed enamel, considering the total amount of the two layers. Fluoride uptake by sound enamel showed a linear dose-response (p = 0.0001), while that by carious slabs showed a polynomial one (p < 0.0001). The results suggest that the in vitro model of reactivity used in the present study is appropriate to evaluate dose-response between fluoride present in aqueous solution and that incorporated by either sound or carious bovine dental enamel. UNITERMS: fluoride; dental enamel; reactivity; dose-response; in vitro study.Modelos in vitro para avaliação da reatividade do fluoreto (F) devem apresentar resposta dose-efeito. Dessa forma, o objetivo desse estudo foi avaliar a relação dose-resposta do fluoreto presente em solução aquosa com o esmalte dental bovino. Cento e vinte blocos de esmalte bovino (5 × 5 × 2 mm), 60 hígidos e 60 com lesão artificial de cárie, foram submetidos durante 10 minutos à água destilada e deionizada (controle negativo) e soluções aquosas contendo 50, 100, 200 ou 400 μg F/mL. Cada grupo experimental recebeu 12 blocos hígidos e 12 blocos com lesão artificial de cárie. Duas camadas consecutivas de esmalte dental foram removidas de todos os blocos dentais por meio de ataque ácido e o fluoreto extraído foi determinado com eletrodo específico. Os resultados de fluoreto incorporado foram expressos em μg por g de esmalte removido, considerando a quantidade total das duas camadas. A incorporação de fluoreto pelo esmalte hígido mostrou uma relação dose-resposta linear (p = 0,0001), enquanto que os blocos com lesão de cárie mostraram relação polinomial quadrática (p < 0,0001). Os resultados sugerem que o modelo in vitro de reatividade empregado no presente estudo é apropriado para avaliar a relação dose-resposta entre o fluoreto em solução aquosa e aquele incorporado pelo esmalte dental bovino hígido ou com lesão artificial de cárie. UNITERMOS: fluoreto; esmalte dental; reatividade; dose-resposta; estudo in vitro.EDIPUCRS - Editora Universitária da PUCRS2007-03-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed Articleapplication/pdfhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/1218Revista Odonto Ciência; Vol. 22 No. 55 (2007); 10 - 15Revista Odonto Ciência; v. 22 n. 55 (2007); 10 - 151980-65230102-9460reponame:Revista odonto ciência (Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/1218/969Arthur, Rodrigo AlexGiancristófaro, MagdaTabchoury, Cínthia Pereira MachadoCury, Jaime Aparecidoinfo:eu-repo/semantics/openAccess2013-11-18T11:34:14Zoai:ojs.revistaseletronicas.pucrs.br:article/1218Revistahttps://revistaseletronicas.pucrs.br/ojs/index.php/foPRIhttps://revistaseletronicas.pucrs.br/ojs/index.php/fo/oai||odontociencia@pucrs.br1980-65230102-9460opendoar:2013-11-18T11:34:14Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv In vitro study of dose-response relationship of fluoride with dental enamel
In vitro study of dose-response relationship of fluoride with dental enamel
title In vitro study of dose-response relationship of fluoride with dental enamel
spellingShingle In vitro study of dose-response relationship of fluoride with dental enamel
Arthur, Rodrigo Alex
title_short In vitro study of dose-response relationship of fluoride with dental enamel
title_full In vitro study of dose-response relationship of fluoride with dental enamel
title_fullStr In vitro study of dose-response relationship of fluoride with dental enamel
title_full_unstemmed In vitro study of dose-response relationship of fluoride with dental enamel
title_sort In vitro study of dose-response relationship of fluoride with dental enamel
author Arthur, Rodrigo Alex
author_facet Arthur, Rodrigo Alex
Giancristófaro, Magda
Tabchoury, Cínthia Pereira Machado
Cury, Jaime Aparecido
author_role author
author2 Giancristófaro, Magda
Tabchoury, Cínthia Pereira Machado
Cury, Jaime Aparecido
author2_role author
author
author
dc.contributor.author.fl_str_mv Arthur, Rodrigo Alex
Giancristófaro, Magda
Tabchoury, Cínthia Pereira Machado
Cury, Jaime Aparecido
description In vitro models for evaluation of fluoride (F) reactivity should present dose-response effect. Thus, the aim of this study was to evaluate the fluoride dose-response present in aqueous solution with bovine dental enamel. A hundred and twenty bovine enamel slabs (5 × 5 × 2 mm), 60 sound and 60 with caries-like lesions, were subjected during 10 min to distilled and deionized water (negative control) and aqueous solutions containing 50, 100, 200 or 400 μg F/mL. Each experimental group received 12 sound and 12 carious slabs. Two consecutive layers of dental enamel were removed from all slabs, by acid etching, and fluoride extracted was determined with specific electrode. The results of fluoride incorporated were expressed in μg per g of removed enamel, considering the total amount of the two layers. Fluoride uptake by sound enamel showed a linear dose-response (p = 0.0001), while that by carious slabs showed a polynomial one (p < 0.0001). The results suggest that the in vitro model of reactivity used in the present study is appropriate to evaluate dose-response between fluoride present in aqueous solution and that incorporated by either sound or carious bovine dental enamel. UNITERMS: fluoride; dental enamel; reactivity; dose-response; in vitro study.
publishDate 2007
dc.date.none.fl_str_mv 2007-03-20
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/1218
url https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/1218
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/ojs/index.php/fo/article/view/1218/969
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
publisher.none.fl_str_mv EDIPUCRS - Editora Universitária da PUCRS
dc.source.none.fl_str_mv Revista Odonto Ciência; Vol. 22 No. 55 (2007); 10 - 15
Revista Odonto Ciência; v. 22 n. 55 (2007); 10 - 15
1980-6523
0102-9460
reponame:Revista odonto ciência (Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Revista odonto ciência (Online)
collection Revista odonto ciência (Online)
repository.name.fl_str_mv Revista odonto ciência (Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv ||odontociencia@pucrs.br
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