Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Scientia Medica (Porto Alegre. Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408 |
Resumo: | AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice. |
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Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extractNatural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extractCopaifera multijugafabaceaecopaibanatural killer cellscarcinomaEhrlich tumorimmunomodulation.AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice.***Atividade natural killer das células esplênicas de camundongos portadores do tumor de Ehrlich tratados com extrato de Copaifera multijuga***OBJETIVOS: O óleo-resina de Copaifera multijuga Hayne é popularmente utilizado na medicina tradicional por suas propriedades anti-inflamatória, antisséptica, antitumoral e antibacteriana. Entretanto, há poucos estudos sobre o efeito dos compostos obtidos da casca da planta. Assim, o objetivo deste trabalho foi avaliar o efeito imunomodulador do extrato etanólico da casca da C. multijuga na atividade natural killer das células esplênicas não aderentes de animais portadores do tumor de Ehrlich.MÉTODOS: Camundongos Swiss machos foram inoculados subcutaneamente com 1 x 106 células do tumor de Ehrlich (grupo Ehrlich e Ehrlich/C. multijuga) ou com solução salina tamponada (grupo controle e C. multijuga) e tratados diariamente (gavagem) com extrato de C. multijuga (200 mg kg-1, 0,1mL cada um, para os grupos Ehrlich/C. multijuga e C. multijuga) ou com solução salina tamponada (grupo controle e grupo Ehrlich). Os quatro grupos experimentais consistiram de oito animais cada e foram organizados em dois conjuntos, um tratado por sete dias e outro tratado por 14 dias, totalizando 64 animais durante todo o experimento. Após 24 horas do término do tratamento, os animais foram eutanasiados para obtenção da suspensão de células esplênicas não aderentes e avaliação da atividade natural killer. Os resultados são apresentados como porcentagem da lise celular das células alvo Yac.1 pelas células esplênicas não aderentes.RESULTADOS: A análise dos resultados demonstrou que aos sete dias de tratamento a C. multijuga aumentou a atividade natural killer no grupo Ehrlich/C. multijuga (21,20±8,89, p<0,05) em comparação ao grupo controle (3,14±2,71, p<0,05), entretanto tal efeito não foi mantido nos grupos tratados por 14 dias (Controle: 6,02±6,98; EHR: 4,82±7,72; C. multijuga: 2,07±2,10; EHR/C. multijuga: 2,01±1,63, p>0,05).CONCLUSÕES: Os resultados demonstraram que o tratamento com o extrato etanólico da casca de C. multijuga favoreceu a atividade natural killer das células esplênicas não aderentes dos animais portadores do tumor de Ehrlich apenas aos sete dias de tratamento.Editora da PUCRS - ediPUCRS2019-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/3240810.15448/1980-6108.2019.1.32408Scientia Medica; Vol. 29 No. 1 (2019); e32408Scientia Medica; v. 29 n. 1 (2019); e324081980-61081806-556210.15448/1980-6108.2019.1reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408/17828Copyright (c) 2019 Scientia Medicahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRodrigues, Paloma Filomena GouveiaAlbiero, Lucinéia ReuseNery, Eduardo FigueredoKelly, Taiany OliveiraDalazen, Jeniffer Charlene SilvaPereira, Débora LinsbinskiSinhorin, Adilson PauloSinhorin, Valéria Dornelles GindriCastoldi, Lindsey2019-08-20T14:02:18Zoai:ojs.revistaseletronicas.pucrs.br:article/32408Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2019-08-20T14:02:18Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract |
title |
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract |
spellingShingle |
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract Rodrigues, Paloma Filomena Gouveia Copaifera multijuga fabaceae copaiba natural killer cells carcinoma Ehrlich tumor immunomodulation. |
title_short |
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract |
title_full |
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract |
title_fullStr |
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract |
title_full_unstemmed |
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract |
title_sort |
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract |
author |
Rodrigues, Paloma Filomena Gouveia |
author_facet |
Rodrigues, Paloma Filomena Gouveia Albiero, Lucinéia Reuse Nery, Eduardo Figueredo Kelly, Taiany Oliveira Dalazen, Jeniffer Charlene Silva Pereira, Débora Linsbinski Sinhorin, Adilson Paulo Sinhorin, Valéria Dornelles Gindri Castoldi, Lindsey |
author_role |
author |
author2 |
Albiero, Lucinéia Reuse Nery, Eduardo Figueredo Kelly, Taiany Oliveira Dalazen, Jeniffer Charlene Silva Pereira, Débora Linsbinski Sinhorin, Adilson Paulo Sinhorin, Valéria Dornelles Gindri Castoldi, Lindsey |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Paloma Filomena Gouveia Albiero, Lucinéia Reuse Nery, Eduardo Figueredo Kelly, Taiany Oliveira Dalazen, Jeniffer Charlene Silva Pereira, Débora Linsbinski Sinhorin, Adilson Paulo Sinhorin, Valéria Dornelles Gindri Castoldi, Lindsey |
dc.subject.por.fl_str_mv |
Copaifera multijuga fabaceae copaiba natural killer cells carcinoma Ehrlich tumor immunomodulation. |
topic |
Copaifera multijuga fabaceae copaiba natural killer cells carcinoma Ehrlich tumor immunomodulation. |
description |
AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-02-27 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408 10.15448/1980-6108.2019.1.32408 |
url |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408 |
identifier_str_mv |
10.15448/1980-6108.2019.1.32408 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408/17828 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Scientia Medica https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Scientia Medica https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da PUCRS - ediPUCRS |
publisher.none.fl_str_mv |
Editora da PUCRS - ediPUCRS |
dc.source.none.fl_str_mv |
Scientia Medica; Vol. 29 No. 1 (2019); e32408 Scientia Medica; v. 29 n. 1 (2019); e32408 1980-6108 1806-5562 10.15448/1980-6108.2019.1 reponame:Scientia Medica (Porto Alegre. Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Scientia Medica (Porto Alegre. Online) |
collection |
Scientia Medica (Porto Alegre. Online) |
repository.name.fl_str_mv |
Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
scientiamedica@pucrs.br || editora.periodicos@pucrs.br |
_version_ |
1809101751937138688 |