Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Paloma Filomena Gouveia
Data de Publicação: 2019
Outros Autores: Albiero, Lucinéia Reuse, Nery, Eduardo Figueredo, Kelly, Taiany Oliveira, Dalazen, Jeniffer Charlene Silva, Pereira, Débora Linsbinski, Sinhorin, Adilson Paulo, Sinhorin, Valéria Dornelles Gindri, Castoldi, Lindsey
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Medica (Porto Alegre. Online)
Texto Completo: https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408
Resumo: AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice.
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spelling Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extractNatural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extractCopaifera multijugafabaceaecopaibanatural killer cellscarcinomaEhrlich tumorimmunomodulation.AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice.***Atividade natural killer das células esplênicas de camundongos portadores do tumor de Ehrlich tratados com extrato de Copaifera multijuga***OBJETIVOS: O óleo-resina de Copaifera multijuga Hayne é popularmente utilizado na medicina tradicional por suas propriedades anti-inflamatória, antisséptica, antitumoral e antibacteriana. Entretanto, há poucos estudos sobre o efeito dos compostos obtidos da casca da planta. Assim, o objetivo deste trabalho foi avaliar o efeito imunomodulador do extrato etanólico da casca da C. multijuga na atividade natural killer das células esplênicas não aderentes de animais portadores do tumor de Ehrlich.MÉTODOS: Camundongos Swiss machos foram inoculados subcutaneamente com 1 x 106 células do tumor de Ehrlich (grupo Ehrlich e Ehrlich/C. multijuga) ou com solução salina tamponada (grupo controle e C. multijuga) e tratados diariamente (gavagem) com extrato de C. multijuga (200 mg kg-1, 0,1mL cada um, para os grupos Ehrlich/C. multijuga e C. multijuga) ou com solução salina tamponada (grupo controle e grupo Ehrlich). Os quatro grupos experimentais consistiram de oito animais cada e foram organizados em dois conjuntos, um tratado por sete dias e outro tratado por 14 dias, totalizando 64 animais durante todo o experimento. Após 24 horas do término do tratamento, os animais foram eutanasiados para obtenção da suspensão de células esplênicas não aderentes e avaliação da atividade natural killer. Os resultados são apresentados como porcentagem da lise celular das células alvo Yac.1 pelas células esplênicas não aderentes.RESULTADOS: A análise dos resultados demonstrou que aos sete dias de tratamento a C. multijuga aumentou a atividade natural killer no grupo Ehrlich/C. multijuga (21,20±8,89, p<0,05) em comparação ao grupo controle (3,14±2,71, p<0,05), entretanto tal efeito não foi mantido nos grupos tratados por 14 dias (Controle: 6,02±6,98; EHR: 4,82±7,72; C. multijuga: 2,07±2,10; EHR/C. multijuga: 2,01±1,63, p>0,05).CONCLUSÕES: Os resultados demonstraram que o tratamento com o extrato etanólico da casca de C. multijuga favoreceu a atividade natural killer das células esplênicas não aderentes dos animais portadores do tumor de Ehrlich apenas aos sete dias de tratamento.Editora da PUCRS - ediPUCRS2019-02-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/3240810.15448/1980-6108.2019.1.32408Scientia Medica; Vol. 29 No. 1 (2019); e32408Scientia Medica; v. 29 n. 1 (2019); e324081980-61081806-556210.15448/1980-6108.2019.1reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408/17828Copyright (c) 2019 Scientia Medicahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessRodrigues, Paloma Filomena GouveiaAlbiero, Lucinéia ReuseNery, Eduardo FigueredoKelly, Taiany OliveiraDalazen, Jeniffer Charlene SilvaPereira, Débora LinsbinskiSinhorin, Adilson PauloSinhorin, Valéria Dornelles GindriCastoldi, Lindsey2019-08-20T14:02:18Zoai:ojs.revistaseletronicas.pucrs.br:article/32408Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2019-08-20T14:02:18Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
title Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
spellingShingle Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
Rodrigues, Paloma Filomena Gouveia
Copaifera multijuga
fabaceae
copaiba
natural killer cells
carcinoma
Ehrlich tumor
immunomodulation.
title_short Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
title_full Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
title_fullStr Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
title_full_unstemmed Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
title_sort Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract
author Rodrigues, Paloma Filomena Gouveia
author_facet Rodrigues, Paloma Filomena Gouveia
Albiero, Lucinéia Reuse
Nery, Eduardo Figueredo
Kelly, Taiany Oliveira
Dalazen, Jeniffer Charlene Silva
Pereira, Débora Linsbinski
Sinhorin, Adilson Paulo
Sinhorin, Valéria Dornelles Gindri
Castoldi, Lindsey
author_role author
author2 Albiero, Lucinéia Reuse
Nery, Eduardo Figueredo
Kelly, Taiany Oliveira
Dalazen, Jeniffer Charlene Silva
Pereira, Débora Linsbinski
Sinhorin, Adilson Paulo
Sinhorin, Valéria Dornelles Gindri
Castoldi, Lindsey
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues, Paloma Filomena Gouveia
Albiero, Lucinéia Reuse
Nery, Eduardo Figueredo
Kelly, Taiany Oliveira
Dalazen, Jeniffer Charlene Silva
Pereira, Débora Linsbinski
Sinhorin, Adilson Paulo
Sinhorin, Valéria Dornelles Gindri
Castoldi, Lindsey
dc.subject.por.fl_str_mv Copaifera multijuga
fabaceae
copaiba
natural killer cells
carcinoma
Ehrlich tumor
immunomodulation.
topic Copaifera multijuga
fabaceae
copaiba
natural killer cells
carcinoma
Ehrlich tumor
immunomodulation.
description AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice.
publishDate 2019
dc.date.none.fl_str_mv 2019-02-27
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408
10.15448/1980-6108.2019.1.32408
url https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408
identifier_str_mv 10.15448/1980-6108.2019.1.32408
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32408/17828
dc.rights.driver.fl_str_mv Copyright (c) 2019 Scientia Medica
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Scientia Medica
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
dc.source.none.fl_str_mv Scientia Medica; Vol. 29 No. 1 (2019); e32408
Scientia Medica; v. 29 n. 1 (2019); e32408
1980-6108
1806-5562
10.15448/1980-6108.2019.1
reponame:Scientia Medica (Porto Alegre. Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Scientia Medica (Porto Alegre. Online)
collection Scientia Medica (Porto Alegre. Online)
repository.name.fl_str_mv Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv scientiamedica@pucrs.br || editora.periodicos@pucrs.br
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