Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012

Detalhes bibliográficos
Autor(a) principal: Paesi, Suelen
Data de Publicação: 2015
Outros Autores: Modena, Nicole, Roesch-Ely, Mariana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Scientia Medica (Porto Alegre. Online)
Texto Completo: https://revistaseletronicas.pucrs.br/scientiamedica/article/view/19519
Resumo: Aims: Rotavirus (RV) and norovirus (NoV) are the most common cause of diarrhea in children aged less than five years throughout the world. However, RV and NoV have frequently been overlooked as pathogens in elderly individuals. This study examined the frequency of RV and NoV among elderly patients with complaints of diarrhea in the city of Caxias do Sul, southern Brazil.Methods: Between 2010 and 2012, stool samples from patients aged 60 years or older with acute gastroenteritis treated at a private healthcare center were analyzed, seeking to identify RV and NoV. RV detection was performed by latex agglutination (LA) methods and polyacrylamide gel electrophoresis (PAGE). One third of the samples were further tested for NoV by reverse transcription polymerase chain reaction (RT-PCR).Results: A total of 145 stool samples from patients aged 60 to 105 years were analyzed. RV was detected in 6/145 (4.14%) and 5/125 (4.00%) of the samples by LA and PAGE, respectively. NoV was detected in 6/51 (11.76%). Mixed infection (RV and NoV) was detected in a single sample: 1/51 (1.96%).Conclusions: This study adds further evidence that viral agents are involved in the etiology of gastroenteritis in the elderly, contributing significantly to the understanding of RV and NoV infections in the mature population.
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spelling Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012Detecção de rotavírus e norovírus na população idosa na cidade de Caxias do Sul, Rio Grande do Sul, Brasil, de 2010 a 2012RotavirusNorovirusElderly PatientsGastroenteritisDiarrhea.Ciências da SaúdeIdosoRotavírusNorovírusGastroenteriteDiarreia.Aims: Rotavirus (RV) and norovirus (NoV) are the most common cause of diarrhea in children aged less than five years throughout the world. However, RV and NoV have frequently been overlooked as pathogens in elderly individuals. This study examined the frequency of RV and NoV among elderly patients with complaints of diarrhea in the city of Caxias do Sul, southern Brazil.Methods: Between 2010 and 2012, stool samples from patients aged 60 years or older with acute gastroenteritis treated at a private healthcare center were analyzed, seeking to identify RV and NoV. RV detection was performed by latex agglutination (LA) methods and polyacrylamide gel electrophoresis (PAGE). One third of the samples were further tested for NoV by reverse transcription polymerase chain reaction (RT-PCR).Results: A total of 145 stool samples from patients aged 60 to 105 years were analyzed. RV was detected in 6/145 (4.14%) and 5/125 (4.00%) of the samples by LA and PAGE, respectively. NoV was detected in 6/51 (11.76%). Mixed infection (RV and NoV) was detected in a single sample: 1/51 (1.96%).Conclusions: This study adds further evidence that viral agents are involved in the etiology of gastroenteritis in the elderly, contributing significantly to the understanding of RV and NoV infections in the mature population.Objetivos: Rotavírus (RV) e norovírus (NoV) são a causa mais comum de diarreia em crianças menores de cinco anos em todo o mundo; entretanto, como patógenos em idosos, esses vírus têm sido pouco investigados. Este estudo examinou a frequência de RV e NoV entre idosos com queixas de diarreia na cidade de Caxias do Sul, no sul do Brasil.Métodos: Entre 2010 e 2012 foram analisadas amostras de fezes de pacientes de um Centro de Atenção à Saúde privado, com idade de 60 anos ou mais, com gastroenterite aguda, buscando-se identificar RV e NoV. A detecção de RV foi realizada pelos métodos Aglutinação em Látex (LA) e Eletroforese em Gel de Poliacrilamida (PAGE). Uma terceira parte das amostras foi ainda testada quanto a NoV por Transcrição Reversa e Reação em Cadeia da Polimerase (RT-PCR).Resultados: Ao todo foram analisadas 145 amostras de fezes de pacientes com idade entre 60 e 105 anos. RV foi detectado em 6/145 (4,14%) e 5/125 (4,00%) das amostras por LA e PAGE, respectivamente. NoV foi detectado em 6/51 (11,76%). Infecção mista (RV e NoV) foi detectada em uma única amostra: 1/51 (1,96%).Conclusões: Este estudo acrescenta mais evidências de que agentes virais estão envolvidos na etiologia da gastroenterite em idosos, e contribui para a compreensão das infecções por RV e NoV na população idosa.Editora da PUCRS - ediPUCRS2015-02-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/1951910.15448/1980-6108.2015.1.19519Scientia Medica; Vol. 25 No. 1 (2015); ID19519Scientia Medica; v. 25 n. 1 (2015); ID195191980-61081806-556210.15448/1980-6108.2015.1reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSenghttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/19519/13501Copyright (c) 2015 Scientia Medicainfo:eu-repo/semantics/openAccessPaesi, SuelenModena, NicoleRoesch-Ely, Mariana2018-08-09T03:04:02Zoai:ojs.revistaseletronicas.pucrs.br:article/19519Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2018-08-09T03:04:02Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012
Detecção de rotavírus e norovírus na população idosa na cidade de Caxias do Sul, Rio Grande do Sul, Brasil, de 2010 a 2012
title Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012
spellingShingle Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012
Paesi, Suelen
Rotavirus
Norovirus
Elderly Patients
Gastroenteritis
Diarrhea.
Ciências da Saúde
Idoso
Rotavírus
Norovírus
Gastroenterite
Diarreia.
title_short Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012
title_full Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012
title_fullStr Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012
title_full_unstemmed Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012
title_sort Detection of rotavirus and norovirus in the elderly population of Caxias do Sul, Rio Grande do Sul, Brazil, from 2010 to 2012
author Paesi, Suelen
author_facet Paesi, Suelen
Modena, Nicole
Roesch-Ely, Mariana
author_role author
author2 Modena, Nicole
Roesch-Ely, Mariana
author2_role author
author
dc.contributor.author.fl_str_mv Paesi, Suelen
Modena, Nicole
Roesch-Ely, Mariana
dc.subject.por.fl_str_mv Rotavirus
Norovirus
Elderly Patients
Gastroenteritis
Diarrhea.
Ciências da Saúde
Idoso
Rotavírus
Norovírus
Gastroenterite
Diarreia.
topic Rotavirus
Norovirus
Elderly Patients
Gastroenteritis
Diarrhea.
Ciências da Saúde
Idoso
Rotavírus
Norovírus
Gastroenterite
Diarreia.
description Aims: Rotavirus (RV) and norovirus (NoV) are the most common cause of diarrhea in children aged less than five years throughout the world. However, RV and NoV have frequently been overlooked as pathogens in elderly individuals. This study examined the frequency of RV and NoV among elderly patients with complaints of diarrhea in the city of Caxias do Sul, southern Brazil.Methods: Between 2010 and 2012, stool samples from patients aged 60 years or older with acute gastroenteritis treated at a private healthcare center were analyzed, seeking to identify RV and NoV. RV detection was performed by latex agglutination (LA) methods and polyacrylamide gel electrophoresis (PAGE). One third of the samples were further tested for NoV by reverse transcription polymerase chain reaction (RT-PCR).Results: A total of 145 stool samples from patients aged 60 to 105 years were analyzed. RV was detected in 6/145 (4.14%) and 5/125 (4.00%) of the samples by LA and PAGE, respectively. NoV was detected in 6/51 (11.76%). Mixed infection (RV and NoV) was detected in a single sample: 1/51 (1.96%).Conclusions: This study adds further evidence that viral agents are involved in the etiology of gastroenteritis in the elderly, contributing significantly to the understanding of RV and NoV infections in the mature population.
publishDate 2015
dc.date.none.fl_str_mv 2015-02-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/19519
10.15448/1980-6108.2015.1.19519
url https://revistaseletronicas.pucrs.br/scientiamedica/article/view/19519
identifier_str_mv 10.15448/1980-6108.2015.1.19519
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/19519/13501
dc.rights.driver.fl_str_mv Copyright (c) 2015 Scientia Medica
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2015 Scientia Medica
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
dc.source.none.fl_str_mv Scientia Medica; Vol. 25 No. 1 (2015); ID19519
Scientia Medica; v. 25 n. 1 (2015); ID19519
1980-6108
1806-5562
10.15448/1980-6108.2015.1
reponame:Scientia Medica (Porto Alegre. Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Scientia Medica (Porto Alegre. Online)
collection Scientia Medica (Porto Alegre. Online)
repository.name.fl_str_mv Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv scientiamedica@pucrs.br || editora.periodicos@pucrs.br
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