Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis

Detalhes bibliográficos
Autor(a) principal: Genske, Rodrigo Daniel
Data de Publicação: 2019
Outros Autores: Loth, Eduardo Alexandre, Cecatto, Vanessa, Gandra, Rinaldo Ferreira, Pilatti, Cleverson Marcelo, Costa, Maricília Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Scientia Medica (Porto Alegre. Online)
Texto Completo: https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705
Resumo: AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model.
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spelling Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensisTerapia fotodinâmica na artrite experimental induzida por Paracoccidioides brasiliensisParacocidioidomycosisArthritis experimentallaser therapyReceptors purinergicBrilliant Blue G.paracoccidioidomicoseartrite experimentalterapia a laserreceptores purinérgicosBrilliant Blue G.AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model.OBJETIVOS: Avaliar a eficácia da terapia fotodinâmica com Brilliant Blue G no tratamento de um modelo experimental de artrite por Paracoccidioides brasiliensis (P. brasiliensis). MÉTODOS: Após a indução de artrite experimental com isolado de P. brasiliensis da linhagem Pb18 nos joelhos de ratos Wistar, os animais foram divididos em grupos e submetidos a terapia fotodinâmica com fotossensibilizador Brilliant Blue G intra-articular e a laserterapia apenas, sem o Brilliant Blue G. Todos os grupos receberam seus respectivos tratamentos do sétimo ao 11º dia. Para análise do edema foi mensurado o diâmetro latero-lateral do joelho de cada animal diariamente e após o período de tratamento os animais foram sacrificados para dissecação do joelho experimental e coleta de sangue para análise por ELISA, a fim de quantificar os níveis de anticorpos anti P. brasiliensis. RESULTADOS: A aplicação da terapia fotodinâmica foi capaz de impedir a formação de edema quando comparado ao controle (p>0,005), bem como a produção de anticorpos anti-Gp-43 de P. brasiliensis (p=0,001). No exame anatomopatológico foi possível observar maior grau de sinovite e maior presença de granulomas com o fungo em seu interior no grupo que não recebeu tratamento quando comparado aos grupos que receberam a terapia fotodinâmica. CONCLUSÕES: A terapia fotodinâmica foi eficaz para atenuar a artrite experimental induzida por P. brasiliensis no modelo articular proposto.Editora da PUCRS - ediPUCRS2019-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/3270510.15448/1980-6108.2019.2.32705Scientia Medica; Vol. 29 No. 2 (2019); e32705Scientia Medica; v. 29 n. 2 (2019); e327051980-61081806-556210.15448/1980-6108.2019.2reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSporhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705/18529Copyright (c) 2019 Scientia Medicahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGenske, Rodrigo DanielLoth, Eduardo AlexandreCecatto, VanessaGandra, Rinaldo FerreiraPilatti, Cleverson MarceloCosta, Maricília Silva2019-10-10T19:30:00Zoai:ojs.revistaseletronicas.pucrs.br:article/32705Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2019-10-10T19:30Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
Terapia fotodinâmica na artrite experimental induzida por Paracoccidioides brasiliensis
title Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
spellingShingle Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
Genske, Rodrigo Daniel
Paracocidioidomycosis
Arthritis experimental
laser therapy
Receptors purinergic
Brilliant Blue G.
paracoccidioidomicose
artrite experimental
terapia a laser
receptores purinérgicos
Brilliant Blue G.
title_short Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
title_full Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
title_fullStr Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
title_full_unstemmed Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
title_sort Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
author Genske, Rodrigo Daniel
author_facet Genske, Rodrigo Daniel
Loth, Eduardo Alexandre
Cecatto, Vanessa
Gandra, Rinaldo Ferreira
Pilatti, Cleverson Marcelo
Costa, Maricília Silva
author_role author
author2 Loth, Eduardo Alexandre
Cecatto, Vanessa
Gandra, Rinaldo Ferreira
Pilatti, Cleverson Marcelo
Costa, Maricília Silva
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Genske, Rodrigo Daniel
Loth, Eduardo Alexandre
Cecatto, Vanessa
Gandra, Rinaldo Ferreira
Pilatti, Cleverson Marcelo
Costa, Maricília Silva
dc.subject.por.fl_str_mv Paracocidioidomycosis
Arthritis experimental
laser therapy
Receptors purinergic
Brilliant Blue G.
paracoccidioidomicose
artrite experimental
terapia a laser
receptores purinérgicos
Brilliant Blue G.
topic Paracocidioidomycosis
Arthritis experimental
laser therapy
Receptors purinergic
Brilliant Blue G.
paracoccidioidomicose
artrite experimental
terapia a laser
receptores purinérgicos
Brilliant Blue G.
description AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705
10.15448/1980-6108.2019.2.32705
url https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705
identifier_str_mv 10.15448/1980-6108.2019.2.32705
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705/18529
dc.rights.driver.fl_str_mv Copyright (c) 2019 Scientia Medica
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Scientia Medica
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
dc.source.none.fl_str_mv Scientia Medica; Vol. 29 No. 2 (2019); e32705
Scientia Medica; v. 29 n. 2 (2019); e32705
1980-6108
1806-5562
10.15448/1980-6108.2019.2
reponame:Scientia Medica (Porto Alegre. Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Scientia Medica (Porto Alegre. Online)
collection Scientia Medica (Porto Alegre. Online)
repository.name.fl_str_mv Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv scientiamedica@pucrs.br || editora.periodicos@pucrs.br
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