Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Scientia Medica (Porto Alegre. Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705 |
Resumo: | AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model. |
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Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensisTerapia fotodinâmica na artrite experimental induzida por Paracoccidioides brasiliensisParacocidioidomycosisArthritis experimentallaser therapyReceptors purinergicBrilliant Blue G.paracoccidioidomicoseartrite experimentalterapia a laserreceptores purinérgicosBrilliant Blue G.AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model.OBJETIVOS: Avaliar a eficácia da terapia fotodinâmica com Brilliant Blue G no tratamento de um modelo experimental de artrite por Paracoccidioides brasiliensis (P. brasiliensis). MÉTODOS: Após a indução de artrite experimental com isolado de P. brasiliensis da linhagem Pb18 nos joelhos de ratos Wistar, os animais foram divididos em grupos e submetidos a terapia fotodinâmica com fotossensibilizador Brilliant Blue G intra-articular e a laserterapia apenas, sem o Brilliant Blue G. Todos os grupos receberam seus respectivos tratamentos do sétimo ao 11º dia. Para análise do edema foi mensurado o diâmetro latero-lateral do joelho de cada animal diariamente e após o período de tratamento os animais foram sacrificados para dissecação do joelho experimental e coleta de sangue para análise por ELISA, a fim de quantificar os níveis de anticorpos anti P. brasiliensis. RESULTADOS: A aplicação da terapia fotodinâmica foi capaz de impedir a formação de edema quando comparado ao controle (p>0,005), bem como a produção de anticorpos anti-Gp-43 de P. brasiliensis (p=0,001). No exame anatomopatológico foi possível observar maior grau de sinovite e maior presença de granulomas com o fungo em seu interior no grupo que não recebeu tratamento quando comparado aos grupos que receberam a terapia fotodinâmica. CONCLUSÕES: A terapia fotodinâmica foi eficaz para atenuar a artrite experimental induzida por P. brasiliensis no modelo articular proposto.Editora da PUCRS - ediPUCRS2019-08-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/3270510.15448/1980-6108.2019.2.32705Scientia Medica; Vol. 29 No. 2 (2019); e32705Scientia Medica; v. 29 n. 2 (2019); e327051980-61081806-556210.15448/1980-6108.2019.2reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSporhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705/18529Copyright (c) 2019 Scientia Medicahttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessGenske, Rodrigo DanielLoth, Eduardo AlexandreCecatto, VanessaGandra, Rinaldo FerreiraPilatti, Cleverson MarceloCosta, Maricília Silva2019-10-10T19:30:00Zoai:ojs.revistaseletronicas.pucrs.br:article/32705Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2019-10-10T19:30Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis Terapia fotodinâmica na artrite experimental induzida por Paracoccidioides brasiliensis |
title |
Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis |
spellingShingle |
Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis Genske, Rodrigo Daniel Paracocidioidomycosis Arthritis experimental laser therapy Receptors purinergic Brilliant Blue G. paracoccidioidomicose artrite experimental terapia a laser receptores purinérgicos Brilliant Blue G. |
title_short |
Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis |
title_full |
Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis |
title_fullStr |
Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis |
title_full_unstemmed |
Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis |
title_sort |
Photodynamic therapy in experimental arthritis induced by Paracoccidioides brasiliensis |
author |
Genske, Rodrigo Daniel |
author_facet |
Genske, Rodrigo Daniel Loth, Eduardo Alexandre Cecatto, Vanessa Gandra, Rinaldo Ferreira Pilatti, Cleverson Marcelo Costa, Maricília Silva |
author_role |
author |
author2 |
Loth, Eduardo Alexandre Cecatto, Vanessa Gandra, Rinaldo Ferreira Pilatti, Cleverson Marcelo Costa, Maricília Silva |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Genske, Rodrigo Daniel Loth, Eduardo Alexandre Cecatto, Vanessa Gandra, Rinaldo Ferreira Pilatti, Cleverson Marcelo Costa, Maricília Silva |
dc.subject.por.fl_str_mv |
Paracocidioidomycosis Arthritis experimental laser therapy Receptors purinergic Brilliant Blue G. paracoccidioidomicose artrite experimental terapia a laser receptores purinérgicos Brilliant Blue G. |
topic |
Paracocidioidomycosis Arthritis experimental laser therapy Receptors purinergic Brilliant Blue G. paracoccidioidomicose artrite experimental terapia a laser receptores purinérgicos Brilliant Blue G. |
description |
AIMS: To evaluate the effectiveness of photodynamic therapy with Brilliant Blue G in the treatment of an experimental model of arthritis by Paracoccidioides brasiliensis (P. brasiliensis). METHODS: After the induction of experimental arthritis with isolated from P. brasiliensis of lineage Pb18 in the knees of Wistar rats, the animals were divided into groups and submitted to photodynamic therapy with intra-articular Brilliant Blue G photosensitizer and laser therapy only, without Brilliant Blue G. All groups received their respective treatments from the seventh to the 11th day. For edema analysis, the knee lateral-lateral diameter of each animal was measured daily and after the treatment period the animals were sacrificed for experimental knee dissection and blood collection for analysis by ELISA, in order to quantify levels of anti-P. brasiliensis antibodies. RESULTS: The results showed that the application of photodynamic therapy was able to prevent the formation of edema when compared to the control (p>0.005), as well as the production of anti-Gp-43 antibodies from P. brasiliensis (p=0.001). In the anatomopathological examination it was possible to observe a higher degree of synovitis and a greater presence of granulomas with the fungus inside the group that did not receive treatment when compared to the groups that received the photodynamic therapy. CONCLUSIONS: Photodynamic therapy was effective in attenuating the experimental arthritis induced by P. brasiliensis in the proposed joint model. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-22 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705 10.15448/1980-6108.2019.2.32705 |
url |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705 |
identifier_str_mv |
10.15448/1980-6108.2019.2.32705 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/32705/18529 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Scientia Medica https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Scientia Medica https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Editora da PUCRS - ediPUCRS |
publisher.none.fl_str_mv |
Editora da PUCRS - ediPUCRS |
dc.source.none.fl_str_mv |
Scientia Medica; Vol. 29 No. 2 (2019); e32705 Scientia Medica; v. 29 n. 2 (2019); e32705 1980-6108 1806-5562 10.15448/1980-6108.2019.2 reponame:Scientia Medica (Porto Alegre. Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Scientia Medica (Porto Alegre. Online) |
collection |
Scientia Medica (Porto Alegre. Online) |
repository.name.fl_str_mv |
Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
scientiamedica@pucrs.br || editora.periodicos@pucrs.br |
_version_ |
1809101751946575872 |