Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Scientia Medica (Porto Alegre. Online) |
Texto Completo: | https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660 |
Resumo: | Aims: To implant and optimize the semi-nested polimerase chain reaction (PCR) to identify and differentiate BK and JC virus. Methods: Nine clinical (urine and liquor) samples have been tested with semi-nested PCR. In order to optimize this reaction, different conditions have been tested. Results: Specificity has been confirmed by using JC and BK positive controls. When tested with semi-nested PCR, the clinical samples showed the following results: 100% (nine samples) positive from BK virus, and 66.6% (six samples) positive from JC virus. Conclusions: The semi-nested PCR reaction, specific for identification of polyomavirus, was optimized for acting as a tool for better follow-up of immunocompromised patients. KEY WORDS: MOLECULAR DIAGNOSTIC TECHNIQUES; JC VIRUS; BK VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS; IMMUNOSUPPRESSION. |
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Scientia Medica (Porto Alegre. Online) |
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Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]Proposta de reação em cadeia da polimerase (PCR) semi-nested para pesquisa e diferenciação dos vírus BK e JCDiagnóstico molecular. Poliomavírus. Imunossuprimidos. ImunocomprometidosAims: To implant and optimize the semi-nested polimerase chain reaction (PCR) to identify and differentiate BK and JC virus. Methods: Nine clinical (urine and liquor) samples have been tested with semi-nested PCR. In order to optimize this reaction, different conditions have been tested. Results: Specificity has been confirmed by using JC and BK positive controls. When tested with semi-nested PCR, the clinical samples showed the following results: 100% (nine samples) positive from BK virus, and 66.6% (six samples) positive from JC virus. Conclusions: The semi-nested PCR reaction, specific for identification of polyomavirus, was optimized for acting as a tool for better follow-up of immunocompromised patients. KEY WORDS: MOLECULAR DIAGNOSTIC TECHNIQUES; JC VIRUS; BK VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS; IMMUNOSUPPRESSION.Objetivos: Implantar e otimizar a reação em cadeia da polimerase (PCR) semi-nested para pesquisa e diferenciação dos vírus BK e JC a partir de amostras clínicas armazenadas. Métodos: Foram testadas 9 amostras clínicas (urina e líquor). As amostras foram submetidas à PCR semi-nested, e foram testadas também com diferentes condições, visando a otimização da reação. Resultados: Primeiramente os controles positivos foram testados quanto à especificidade através de uma PCR direta, e os resultados obtidos confirmaram a especificidade. Quando testadas por PCR semi-nested, as amostras apresentaram os seguintes resultados: 100% (nove amostras) positivas para o vírus BK e 66,6% (seis amostras) positivas para o vírus JC. Conclusões: Otimizou-se a reação de PCR seminested, específica para a identificação dos poliomavírus, para assim atuar como uma ferramenta diagnóstica para melhor acompanhamento de pacientes imunocomprometidos. DESCRITORES: TÉCNICAS DE DIAGNÓSTICO MOLECULAR; REAÇÃO EM CADEIA DA POLIMERASE; POLYOMAVIRUS; VIRUS JC; VIRUS BK; IMUNOSSUPRESSÃO.Editora da PUCRS - ediPUCRS2008-01-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionEstudo diagnósticoapplication/pdfapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660Scientia Medica; Vol. 17 No. 2 (2007); 72-78Scientia Medica; v. 17 n. 2 (2007); 72-781980-61081806-5562reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSporenghttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660/1901https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660/7881Domingues, Juliana Toniettode Paris, FernandaSchmitt, Virginia Minghelliinfo:eu-repo/semantics/openAccess2013-07-17T13:30:50Zoai:ojs.revistaseletronicas.pucrs.br:article/1660Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2013-07-17T13:30:50Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false |
dc.title.none.fl_str_mv |
Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English] Proposta de reação em cadeia da polimerase (PCR) semi-nested para pesquisa e diferenciação dos vírus BK e JC |
title |
Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English] |
spellingShingle |
Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English] Domingues, Juliana Tonietto Diagnóstico molecular. Poliomavírus. Imunossuprimidos. Imunocomprometidos |
title_short |
Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English] |
title_full |
Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English] |
title_fullStr |
Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English] |
title_full_unstemmed |
Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English] |
title_sort |
Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English] |
author |
Domingues, Juliana Tonietto |
author_facet |
Domingues, Juliana Tonietto de Paris, Fernanda Schmitt, Virginia Minghelli |
author_role |
author |
author2 |
de Paris, Fernanda Schmitt, Virginia Minghelli |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Domingues, Juliana Tonietto de Paris, Fernanda Schmitt, Virginia Minghelli |
dc.subject.por.fl_str_mv |
Diagnóstico molecular. Poliomavírus. Imunossuprimidos. Imunocomprometidos |
topic |
Diagnóstico molecular. Poliomavírus. Imunossuprimidos. Imunocomprometidos |
description |
Aims: To implant and optimize the semi-nested polimerase chain reaction (PCR) to identify and differentiate BK and JC virus. Methods: Nine clinical (urine and liquor) samples have been tested with semi-nested PCR. In order to optimize this reaction, different conditions have been tested. Results: Specificity has been confirmed by using JC and BK positive controls. When tested with semi-nested PCR, the clinical samples showed the following results: 100% (nine samples) positive from BK virus, and 66.6% (six samples) positive from JC virus. Conclusions: The semi-nested PCR reaction, specific for identification of polyomavirus, was optimized for acting as a tool for better follow-up of immunocompromised patients. KEY WORDS: MOLECULAR DIAGNOSTIC TECHNIQUES; JC VIRUS; BK VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS; IMMUNOSUPPRESSION. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-01-08 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Estudo diagnóstico |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660 |
url |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660/1901 https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660/7881 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Editora da PUCRS - ediPUCRS |
publisher.none.fl_str_mv |
Editora da PUCRS - ediPUCRS |
dc.source.none.fl_str_mv |
Scientia Medica; Vol. 17 No. 2 (2007); 72-78 Scientia Medica; v. 17 n. 2 (2007); 72-78 1980-6108 1806-5562 reponame:Scientia Medica (Porto Alegre. Online) instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) instacron:PUC_RS |
instname_str |
Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
instacron_str |
PUC_RS |
institution |
PUC_RS |
reponame_str |
Scientia Medica (Porto Alegre. Online) |
collection |
Scientia Medica (Porto Alegre. Online) |
repository.name.fl_str_mv |
Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS) |
repository.mail.fl_str_mv |
scientiamedica@pucrs.br || editora.periodicos@pucrs.br |
_version_ |
1809101748499906560 |