Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]

Detalhes bibliográficos
Autor(a) principal: Domingues, Juliana Tonietto
Data de Publicação: 2008
Outros Autores: de Paris, Fernanda, Schmitt, Virginia Minghelli
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Scientia Medica (Porto Alegre. Online)
Texto Completo: https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660
Resumo: Aims: To implant and optimize the semi-nested polimerase chain reaction (PCR) to identify and differentiate BK and JC virus. Methods: Nine clinical (urine and liquor) samples have been tested with semi-nested PCR. In order to optimize this reaction, different conditions have been tested. Results: Specificity has been confirmed by using JC and BK positive controls. When tested with semi-nested PCR, the clinical samples showed the following results: 100% (nine samples) positive from BK virus, and 66.6% (six samples) positive from JC virus. Conclusions: The semi-nested PCR reaction, specific for identification of polyomavirus, was optimized for acting as a tool for better follow-up of immunocompromised patients. KEY WORDS: MOLECULAR DIAGNOSTIC TECHNIQUES; JC VIRUS; BK VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS; IMMUNOSUPPRESSION.
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spelling Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]Proposta de reação em cadeia da polimerase (PCR) semi-nested para pesquisa e diferenciação dos vírus BK e JCDiagnóstico molecular. Poliomavírus. Imunossuprimidos. ImunocomprometidosAims: To implant and optimize the semi-nested polimerase chain reaction (PCR) to identify and differentiate BK and JC virus. Methods: Nine clinical (urine and liquor) samples have been tested with semi-nested PCR. In order to optimize this reaction, different conditions have been tested. Results: Specificity has been confirmed by using JC and BK positive controls. When tested with semi-nested PCR, the clinical samples showed the following results: 100% (nine samples) positive from BK virus, and 66.6% (six samples) positive from JC virus. Conclusions: The semi-nested PCR reaction, specific for identification of polyomavirus, was optimized for acting as a tool for better follow-up of immunocompromised patients. KEY WORDS: MOLECULAR DIAGNOSTIC TECHNIQUES; JC VIRUS; BK VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS; IMMUNOSUPPRESSION.Objetivos: Implantar e otimizar a reação em cadeia da polimerase (PCR) semi-nested para pesquisa e diferenciação dos vírus BK e JC a partir de amostras clínicas armazenadas. Métodos: Foram testadas 9 amostras clínicas (urina e líquor). As amostras foram submetidas à PCR semi-nested, e foram testadas também com diferentes condições, visando a otimização da reação. Resultados: Primeiramente os controles positivos foram testados quanto à especificidade através de uma PCR direta, e os resultados obtidos confirmaram a especificidade. Quando testadas por PCR semi-nested, as amostras apresentaram os seguintes resultados: 100% (nove amostras) positivas para o vírus BK e 66,6% (seis amostras) positivas para o vírus JC. Conclusões: Otimizou-se a reação de PCR seminested, específica para a identificação dos poliomavírus, para assim atuar como uma ferramenta diagnóstica para melhor acompanhamento de pacientes imunocomprometidos. DESCRITORES: TÉCNICAS DE DIAGNÓSTICO MOLECULAR; REAÇÃO EM CADEIA DA POLIMERASE; POLYOMAVIRUS; VIRUS JC; VIRUS BK; IMUNOSSUPRESSÃO.Editora da PUCRS - ediPUCRS2008-01-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionEstudo diagnósticoapplication/pdfapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660Scientia Medica; Vol. 17 No. 2 (2007); 72-78Scientia Medica; v. 17 n. 2 (2007); 72-781980-61081806-5562reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSporenghttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660/1901https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660/7881Domingues, Juliana Toniettode Paris, FernandaSchmitt, Virginia Minghelliinfo:eu-repo/semantics/openAccess2013-07-17T13:30:50Zoai:ojs.revistaseletronicas.pucrs.br:article/1660Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2013-07-17T13:30:50Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
Proposta de reação em cadeia da polimerase (PCR) semi-nested para pesquisa e diferenciação dos vírus BK e JC
title Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
spellingShingle Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
Domingues, Juliana Tonietto
Diagnóstico molecular. Poliomavírus. Imunossuprimidos. Imunocomprometidos
title_short Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
title_full Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
title_fullStr Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
title_full_unstemmed Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
title_sort Proposal of semi-nested polimerase chain reaction (PCR) for diagnosing and differentiating BK and JC virus [Abstract in English]
author Domingues, Juliana Tonietto
author_facet Domingues, Juliana Tonietto
de Paris, Fernanda
Schmitt, Virginia Minghelli
author_role author
author2 de Paris, Fernanda
Schmitt, Virginia Minghelli
author2_role author
author
dc.contributor.author.fl_str_mv Domingues, Juliana Tonietto
de Paris, Fernanda
Schmitt, Virginia Minghelli
dc.subject.por.fl_str_mv Diagnóstico molecular. Poliomavírus. Imunossuprimidos. Imunocomprometidos
topic Diagnóstico molecular. Poliomavírus. Imunossuprimidos. Imunocomprometidos
description Aims: To implant and optimize the semi-nested polimerase chain reaction (PCR) to identify and differentiate BK and JC virus. Methods: Nine clinical (urine and liquor) samples have been tested with semi-nested PCR. In order to optimize this reaction, different conditions have been tested. Results: Specificity has been confirmed by using JC and BK positive controls. When tested with semi-nested PCR, the clinical samples showed the following results: 100% (nine samples) positive from BK virus, and 66.6% (six samples) positive from JC virus. Conclusions: The semi-nested PCR reaction, specific for identification of polyomavirus, was optimized for acting as a tool for better follow-up of immunocompromised patients. KEY WORDS: MOLECULAR DIAGNOSTIC TECHNIQUES; JC VIRUS; BK VIRUS; POLYMERASE CHAIN REACTION; POLYOMAVIRUS; IMMUNOSUPPRESSION.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-08
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Estudo diagnóstico
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660
url https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660/1901
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/1660/7881
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
dc.source.none.fl_str_mv Scientia Medica; Vol. 17 No. 2 (2007); 72-78
Scientia Medica; v. 17 n. 2 (2007); 72-78
1980-6108
1806-5562
reponame:Scientia Medica (Porto Alegre. Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Scientia Medica (Porto Alegre. Online)
collection Scientia Medica (Porto Alegre. Online)
repository.name.fl_str_mv Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv scientiamedica@pucrs.br || editora.periodicos@pucrs.br
_version_ 1809101748499906560

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