Antiviral activity of dermaseptin 01 against Dengue virus type 2, Herpes simplex virus type 1 and Vaccinia virus[Abstract in English]

• Autor(a) principal: Cardoso, João Luiz Macedo de Souza
• Data de Publicação: 2013
• Outros Autores: Soares, Maria José dos Santos, Leite, José Roberto Souza Almeida, Malaquias, Luiz Cosme Cotta, Coelho, Luiz Felipe Leomil
• Tipo de documento: Artigo
• Idioma: por; eng
• Título da fonte: Scientia Medica (Porto Alegre. Online)
• Texto Completo: https://revistaseletronicas.pucrs.br/scientiamedica/article/view/12751
• topic: PRODUCTS WITH ANTIMICROBIAL ACTION; ANTIVIRAL AGENTS; DENGUE VIRUS; VACCINIA VIRUS; HERPES SIMPLEX; ANPHIBIANS.; PRODUTOS COM AÇÃO ANTIMICROBIANA; ANTIVIRAIS; VÍRUS DA DENGUE; VÍRUS VACCINIA; HERPES SIMPLES; ANFÍBIOS

AIMS: Determine the in vitro antiviral activity of dermaseptin-1 an antimicrobial peptide discovered in the skin secretion of Phyllomedusa hypochondrialis frogs against Herpes simplex virus type 1, Vaccinia virus and Dengue virus type 2. METHODS: The Peptide dermaseptin-1 was used for the cytotoxic assays using Vero, LLCMK2 and C6/36 cells. The antiviral activity was evaluated using (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide colorimetric assay for Herpes simplex virus type 1 and Vaccinia virus in VERO cells and for Dengue virus type 2 in LLCMK2 cells. The antiviral activity of dermaseptin-1was also evaluated in C6/36 cells infected with Dengue virus type 2 by cytopathic effect reduction. RESULTS: The 50% cytotoxicity concentration of dermaseptin-1 was 105 µg/ml in insect cells (C6/36) and more than 1000 µg/ml in mammalian cells (VERO and LLCMK2). DS-01 displayed antiviral effect only against Dengue virus type 2 with a 50% effective concentration of 15 µg/ml in C6/36 cells and 60 µg/ml in LLCMK2 cells. CONCLUSIONS: These data suggested that dermaseptin 01 have an in vitro antiviral action against Dengue virus type 2 but not against Herpes simplex virus type 1 or Vaccinia virus.