In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]

Detalhes bibliográficos
Autor(a) principal: Garcia, Charlene Silvestrin Celi
Data de Publicação: 2012
Outros Autores: Lambert, Ana Paula Franco, Henriques, João Antonio Pêgas, Ely, Mariana Roesch
Tipo de documento: Artigo
Idioma: por
eng
Título da fonte: Scientia Medica (Porto Alegre. Online)
Texto Completo: https://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790
Resumo: AIMS: To determine cell viability compared to the biological effect of Salvia officinalis L. extract on cultured tumor cells of the larynx (Hep-2) and in human hepatoma cells (HepG2) METHODS: Tumor cells Hep-2 and HepG2 were grown in DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10% inactivated fetal bovine serum and 1% antibiotics (Penicillin/Streptomycin), in incubator at 37°C and humidified with 5% CO2. In the second step they were plated (5 x 104 cells/mL) in 96 well plates during 24 hours to obtain 60-70% confluency, and treated with the hydroalcoholic extract of Salvia officinalis L., negative control with 80% ethanol (v/v) and positive control of hydrogen peroxide (H2O2), t-butyl peroxide hidroxy (TBO), doxorubicin and cisplatin. Cell viability was determined by MTT reduction (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Results were analyzed by Tukey test (p≤0,05) using the SPSS v.19. RESULTS: the hydroalcoholic extract of Salvia officinalis L. showed cytotoxic activity to tumor cells Hep-2 IC50 0,38 ± 0.02 mg/mL for HepG2 IC50 0,54 ± 0.03 mg/mL compared to negative control, staying above the IC50 obtained for their positive controls. Cisplatin showed IC50 below the extract of sage, but to obtain their IC50 was increased exposure time in six hours. CONCLUSIONS: the results of this study suggest that the extract of Salvia officinalis L. has biological activity against tumor cell; further studies are needed to confirm its efficacy and its potential role in cancer treatment.
id PUC_RS-25_fedbfc4e69f6b605cb421f6805c181ea
oai_identifier_str oai:ojs.revistaseletronicas.pucrs.br:article/10790
network_acronym_str PUC_RS-25
network_name_str Scientia Medica (Porto Alegre. Online)
repository_id_str
spelling In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]Avaliação in vitro do potencial biológico da Salvia officinalis L. em células tumoraisSALVIA OFFICINALISCELL SURVIVALANTINEOPLASTIC AGENTSTHERAPY.Salvia officinalisSOBREVIVÊNCIA CELULARANTINEOPLÁSICOSTERAPÊUTICA.AIMS: To determine cell viability compared to the biological effect of Salvia officinalis L. extract on cultured tumor cells of the larynx (Hep-2) and in human hepatoma cells (HepG2) METHODS: Tumor cells Hep-2 and HepG2 were grown in DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10% inactivated fetal bovine serum and 1% antibiotics (Penicillin/Streptomycin), in incubator at 37°C and humidified with 5% CO2. In the second step they were plated (5 x 104 cells/mL) in 96 well plates during 24 hours to obtain 60-70% confluency, and treated with the hydroalcoholic extract of Salvia officinalis L., negative control with 80% ethanol (v/v) and positive control of hydrogen peroxide (H2O2), t-butyl peroxide hidroxy (TBO), doxorubicin and cisplatin. Cell viability was determined by MTT reduction (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Results were analyzed by Tukey test (p≤0,05) using the SPSS v.19. RESULTS: the hydroalcoholic extract of Salvia officinalis L. showed cytotoxic activity to tumor cells Hep-2 IC50 0,38 ± 0.02 mg/mL for HepG2 IC50 0,54 ± 0.03 mg/mL compared to negative control, staying above the IC50 obtained for their positive controls. Cisplatin showed IC50 below the extract of sage, but to obtain their IC50 was increased exposure time in six hours. CONCLUSIONS: the results of this study suggest that the extract of Salvia officinalis L. has biological activity against tumor cell; further studies are needed to confirm its efficacy and its potential role in cancer treatment.OBJETIVOS: Determinar a viabilidade celular frente ao efeito biológico do extrato hidroalcoólico de Salvia officinalis L. em culturas de células tumorais de laringe (Hep-2) e células de hepatoma humano (HepG2). MÉTODOS: Células tumorais Hep-2 e HepG2 foram cultivadas em meio DMEM (Dulbecco’s Modified Eagle Médium) suplementado com 10% soro fetal bovino inativado e 1% de antibiótico (Penicilina/Estreptomicina) em estufa a 37°C e atmosfera umidificada com 5% de CO2. Na segunda etapa foram semeadas (5 x 104 células/mL) em placas de 96 poços durante o período de 24 horas até obtenção de 60-70% de confluência e realizou-se o tratamento das células com extrato hidroalcoólico de Salvia officinalis L., controle negativo de etanol 80% (v/v) e controles positivos de peróxido de hidrogênio (H2O2), t-butil hidroxi peroxido (TBO), doxorrubicina e cisplatina. A viabilidade celular foi determinada pela redução do MTT (brometo de 3- (4,5 dimetiltiazol-2-il)-2,5-difeniltetrazolio). Os resultados foram analisados pelo teste de Tukey no programa SPSS v.19. RESULTADOS: O extrato hidroalcoólico de Salvia officinalis L. mostrou atividade citotóxica para células tumorais Hep-2 IC50 0,38±0,02 mg/mL e para HepG2 IC50 0,54±0,03 mg/mL em comparação ao controle negativo, ficando acima do IC50 obtido para seus controles positivos. A cisplatina apresentou IC50 abaixo do extrato de sálvia, porém para a obtenção do seu IC50 aumentou-se o tempo de exposição em seis horas. CONCLUSÕES: sugere-se que o extrato de Salvia officinalis L. tem atividade biológica em células tumorais, podendo ser objetivo de mais estudos com a finalidade de comprovar sua eficácia como possível agente para o tratamento do câncer.Editora da PUCRS - ediPUCRS2012-07-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790Scientia Medica; Vol. 22 No. 3 (2012); 131-137Scientia Medica; v. 22 n. 3 (2012); 131-1371980-61081806-5562reponame:Scientia Medica (Porto Alegre. Online)instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSporenghttps://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790/8178https://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790/8179https://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790/8180Garcia, Charlene Silvestrin CeliLambert, Ana Paula FrancoHenriques, João Antonio PêgasEly, Mariana Roeschinfo:eu-repo/semantics/openAccess2013-07-15T18:40:59Zoai:ojs.revistaseletronicas.pucrs.br:article/10790Revistahttps://revistaseletronicas.pucrs.br/scientiamedica/PUBhttps://revistaseletronicas.pucrs.br/scientiamedica/oaiscientiamedica@pucrs.br || editora.periodicos@pucrs.br1980-61081806-5562opendoar:2013-07-15T18:40:59Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.none.fl_str_mv In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]
Avaliação in vitro do potencial biológico da Salvia officinalis L. em células tumorais
title In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]
spellingShingle In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]
Garcia, Charlene Silvestrin Celi
SALVIA OFFICINALIS
CELL SURVIVAL
ANTINEOPLASTIC AGENTS
THERAPY.
Salvia officinalis
SOBREVIVÊNCIA CELULAR
ANTINEOPLÁSICOS
TERAPÊUTICA.
title_short In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]
title_full In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]
title_fullStr In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]
title_full_unstemmed In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]
title_sort In vitro evaluation of the biological potential of Salvia officinalis L. in tumor cells [Abstract in English]
author Garcia, Charlene Silvestrin Celi
author_facet Garcia, Charlene Silvestrin Celi
Lambert, Ana Paula Franco
Henriques, João Antonio Pêgas
Ely, Mariana Roesch
author_role author
author2 Lambert, Ana Paula Franco
Henriques, João Antonio Pêgas
Ely, Mariana Roesch
author2_role author
author
author
dc.contributor.author.fl_str_mv Garcia, Charlene Silvestrin Celi
Lambert, Ana Paula Franco
Henriques, João Antonio Pêgas
Ely, Mariana Roesch
dc.subject.por.fl_str_mv SALVIA OFFICINALIS
CELL SURVIVAL
ANTINEOPLASTIC AGENTS
THERAPY.
Salvia officinalis
SOBREVIVÊNCIA CELULAR
ANTINEOPLÁSICOS
TERAPÊUTICA.
topic SALVIA OFFICINALIS
CELL SURVIVAL
ANTINEOPLASTIC AGENTS
THERAPY.
Salvia officinalis
SOBREVIVÊNCIA CELULAR
ANTINEOPLÁSICOS
TERAPÊUTICA.
description AIMS: To determine cell viability compared to the biological effect of Salvia officinalis L. extract on cultured tumor cells of the larynx (Hep-2) and in human hepatoma cells (HepG2) METHODS: Tumor cells Hep-2 and HepG2 were grown in DMEM (Dulbecco's Modified Eagle Medium) supplemented with 10% inactivated fetal bovine serum and 1% antibiotics (Penicillin/Streptomycin), in incubator at 37°C and humidified with 5% CO2. In the second step they were plated (5 x 104 cells/mL) in 96 well plates during 24 hours to obtain 60-70% confluency, and treated with the hydroalcoholic extract of Salvia officinalis L., negative control with 80% ethanol (v/v) and positive control of hydrogen peroxide (H2O2), t-butyl peroxide hidroxy (TBO), doxorubicin and cisplatin. Cell viability was determined by MTT reduction (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide). Results were analyzed by Tukey test (p≤0,05) using the SPSS v.19. RESULTS: the hydroalcoholic extract of Salvia officinalis L. showed cytotoxic activity to tumor cells Hep-2 IC50 0,38 ± 0.02 mg/mL for HepG2 IC50 0,54 ± 0.03 mg/mL compared to negative control, staying above the IC50 obtained for their positive controls. Cisplatin showed IC50 below the extract of sage, but to obtain their IC50 was increased exposure time in six hours. CONCLUSIONS: the results of this study suggest that the extract of Salvia officinalis L. has biological activity against tumor cell; further studies are needed to confirm its efficacy and its potential role in cancer treatment.
publishDate 2012
dc.date.none.fl_str_mv 2012-07-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790
url https://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790
dc.language.iso.fl_str_mv por
eng
language por
eng
dc.relation.none.fl_str_mv https://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790/8178
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790/8179
https://revistaseletronicas.pucrs.br/scientiamedica/article/view/10790/8180
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
publisher.none.fl_str_mv Editora da PUCRS - ediPUCRS
dc.source.none.fl_str_mv Scientia Medica; Vol. 22 No. 3 (2012); 131-137
Scientia Medica; v. 22 n. 3 (2012); 131-137
1980-6108
1806-5562
reponame:Scientia Medica (Porto Alegre. Online)
instname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron:PUC_RS
instname_str Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
instacron_str PUC_RS
institution PUC_RS
reponame_str Scientia Medica (Porto Alegre. Online)
collection Scientia Medica (Porto Alegre. Online)
repository.name.fl_str_mv Scientia Medica (Porto Alegre. Online) - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)
repository.mail.fl_str_mv scientiamedica@pucrs.br || editora.periodicos@pucrs.br
_version_ 1809101749627125760

Registros relacionados