Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)

Detalhes bibliográficos
Autor(a) principal: Gusso, Darlan
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da PUC_RS
Texto Completo: http://tede2.pucrs.br/tede2/handle/tede/7950
Resumo: Mosquitoes are responsible for the transmission of various pathogens. Over the past three years, the world has witnessed epidemics affecting all age groups, such as dengue fever, yellow fever, chikungunya and zika virus. To control and eliminate Aedes vectors responsible for the transmission of these diseases, it is necessary to use larvicides and insecticides such as pyriproxyfen. Pyriproxyfen is a larvicide used to control mosquitoes, mainly of the genus Aedes. This study evaluated the effects of pyriproxyfen on survival, morphology, behavior, and endocrine parameters at different stages of zebrafish development. We have demonstrated that pyriproxyfen can cause changes in the survival rate, behavior and morphology of exposed larvae, adults exposed at the larval stage, and adult offspring of exposed parents. Larvae exposed to pyriproxyfen for 1-96 hpf (hours post-fertilization) showed a decrease in survival rate, heart rate 3 dpf (days post-fertilization), and body length at 5 and 8 dpf. The distance traveled and the mean velocities of the exposed larvae were reduced in comparison to the vehicle group (1% DMSO). Therefore, pyriproxyfen changes the morphology and behavior of zebrafish in early stages of development and may affect the next generations. However, when the exposed larvae were maintained until the adult stage (6 months), we did not observe differences in locomotor parameters. Parental exposure to pyriproxyfen induced decrease in locomotion of zebrafish adult offspring as well as an anxiolytic-like behavior. We also tested pyriproxyfen exposure for 96 hours in adult zebrafish and the locomotion, anxiety, memory and endocrine parameters were analyzed as well as the gene expression of the glucocorticoid receptor (GR) and corticotropin-releasing factor (CRF). These results showed that there was no significant difference in locomotion, anxiety and endocrine parameters. In addition, there was an impairment of habituation memory in adult zebrafish exposed to pyriproxyfen. Therefore, it is important to control the use of larvicides due to their toxic effects on non-target species. Our findings demonstrated the importance of studies related to the use of larvicides, since they are potential causes of morphological and behavioral alterations in aquatic species, such as zebrafish.
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spelling Bonan, Carla Denisehttp://lattes.cnpq.br/8058052532279136http://lattes.cnpq.br/1278147802553764Gusso, Darlan2018-04-19T16:43:13Z2018-02-21http://tede2.pucrs.br/tede2/handle/tede/7950Mosquitoes are responsible for the transmission of various pathogens. Over the past three years, the world has witnessed epidemics affecting all age groups, such as dengue fever, yellow fever, chikungunya and zika virus. To control and eliminate Aedes vectors responsible for the transmission of these diseases, it is necessary to use larvicides and insecticides such as pyriproxyfen. Pyriproxyfen is a larvicide used to control mosquitoes, mainly of the genus Aedes. This study evaluated the effects of pyriproxyfen on survival, morphology, behavior, and endocrine parameters at different stages of zebrafish development. We have demonstrated that pyriproxyfen can cause changes in the survival rate, behavior and morphology of exposed larvae, adults exposed at the larval stage, and adult offspring of exposed parents. Larvae exposed to pyriproxyfen for 1-96 hpf (hours post-fertilization) showed a decrease in survival rate, heart rate 3 dpf (days post-fertilization), and body length at 5 and 8 dpf. The distance traveled and the mean velocities of the exposed larvae were reduced in comparison to the vehicle group (1% DMSO). Therefore, pyriproxyfen changes the morphology and behavior of zebrafish in early stages of development and may affect the next generations. However, when the exposed larvae were maintained until the adult stage (6 months), we did not observe differences in locomotor parameters. Parental exposure to pyriproxyfen induced decrease in locomotion of zebrafish adult offspring as well as an anxiolytic-like behavior. We also tested pyriproxyfen exposure for 96 hours in adult zebrafish and the locomotion, anxiety, memory and endocrine parameters were analyzed as well as the gene expression of the glucocorticoid receptor (GR) and corticotropin-releasing factor (CRF). These results showed that there was no significant difference in locomotion, anxiety and endocrine parameters. In addition, there was an impairment of habituation memory in adult zebrafish exposed to pyriproxyfen. Therefore, it is important to control the use of larvicides due to their toxic effects on non-target species. Our findings demonstrated the importance of studies related to the use of larvicides, since they are potential causes of morphological and behavioral alterations in aquatic species, such as zebrafish.Mosquitos são responsáveis pela transmissão de vários agentes patogênicos. Nos últimos três anos, o mundo testemunhou epidemias que afetam todas as faixas etárias, tais como dengue, febre amarela, chikungunya e zika vírus. Para controlar e eliminar vetores do gênero Aedes, responsável pela transmissão destas doenças, é necessário usar larvicidas e inseticidas como o piriproxifeno. O piriproxifeno é um larvicida usado para eliminar mosquitos, principalmente do gênero Aedes. Este estudo avaliou os efeitos do piriproxifeno sobre a sobrevivência, morfologia, comportamento e parâmetros endócrinos em diferentes estágios de desenvolvimento de peixe-zebra. Nós demonstramos que o piriproxifeno pode causar alterações na taxa de sobrevivência, comportamento e morfologia de larvas expostas, adultos expostos no estágio larval e descendentes adultos de pais expostos. As larvas expostas ao piriproxifeno durante 1-96 hpf (horas pós-fertilização) mostraram uma diminuição na taxa de sobrevivência, frequência cardíaca 3 dpf e comprimento corporal nos 5 e 8 dpf (dias pós- fertilização). A distância percorrida e a velocidade média das larvas expostas foram reduzidas em relação ao grupo veículo (1% de DMSO). Portanto, o piriproxifeno altera a morfologia e o comportamento do peixe-zebra em estágios iniciais de desenvolvimento e pode afetar as próximas gerações. No entanto, quando as larvas expostas foram mantidas até o estágio adulto (6 meses), não observamos diferenças nos parâmetros locomotores. A exposição parental ao piriproxifeno induziu uma diminuição na locomoção da prole adulta, bem como um comportamento do tipo ansiolítico. Nós também avaliamos o efeito da exposição ao piriproxifeno em peixe-zebra na fase adulta durante 96 horas sobre a locomoção, ansiedade, memória e parâmetros endócrinos, como expressão gênica do receptor de glicocorticoide (GR) e fator de liberação de corticotrofina (CRF). Os resultados demonstraram que não houve diferença significativa na locomoção, ansiedade e parâmetros endócrinos. Além disso, houve um prejuízo da memória de habituação do peixe-zebra adulto exposto à piriproxifeno. Portanto, é importante controlar o uso de larvicidas devido aos seus efeitos tóxicos em espécies não-alvo. Nossos achados demonstram a importância de estudos relacionados com a utilização de larvicidas, uma vez que são potenciais causadores de alterações morfológicas e comportamentais em espécies aquáticas, como o peixe-zebra.Submitted by PPG Biologia Celular e Molecular (bcm@pucrs.br) on 2018-04-04T12:33:24Z No. of bitstreams: 1 DARLAN_GUSSO_DIS.pdf: 1507228 bytes, checksum: e2389f511956dbb64bb7b767d8f73e40 (MD5)Approved for entry into archive by Caroline Xavier (caroline.xavier@pucrs.br) on 2018-04-19T16:26:33Z (GMT) No. of bitstreams: 1 DARLAN_GUSSO_DIS.pdf: 1507228 bytes, checksum: e2389f511956dbb64bb7b767d8f73e40 (MD5)Made available in DSpace on 2018-04-19T16:43:13Z (GMT). No. of bitstreams: 1 DARLAN_GUSSO_DIS.pdf: 1507228 bytes, checksum: e2389f511956dbb64bb7b767d8f73e40 (MD5) Previous issue date: 2018-02-21Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESapplication/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/171570/DIS_DARLAN_GUSSO_CONFIDENCIAL.pdf.jpghttp://tede2.pucrs.br:80/tede2/retrieve/178555/DIS_DARLAN_GUSSO_COMPLETO.pdf.jpgporPontifícia Universidade Católica do Rio Grande do SulPrograma de Pós-Graduação em Biologia Celular e MolecularPUCRSBrasilEscola de CiênciasComportamentoMemóriaLarvicidaPiriproxifenoPeixe-zebraCIENCIAS BIOLOGICAS::BIOLOGIA GERALEfeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisTrabalho será publicado como artigo ou livro24 meses19/04/20208198246930096637360500500600-16345593859312446972075167498588264571info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSORIGINALDIS_DARLAN_GUSSO_COMPLETO.pdfDIS_DARLAN_GUSSO_COMPLETO.pdfapplication/pdf1507228http://tede2.pucrs.br/tede2/bitstream/tede/7950/5/DIS_DARLAN_GUSSO_COMPLETO.pdfe2389f511956dbb64bb7b767d8f73e40MD55THUMBNAILDIS_DARLAN_GUSSO_CONFIDENCIAL.pdf.jpgDIS_DARLAN_GUSSO_CONFIDENCIAL.pdf.jpgimage/jpeg4108http://tede2.pucrs.br/tede2/bitstream/tede/7950/4/DIS_DARLAN_GUSSO_CONFIDENCIAL.pdf.jpg2429f5924b48ddcd8505ad47b63c24e3MD54DIS_DARLAN_GUSSO_COMPLETO.pdf.jpgDIS_DARLAN_GUSSO_COMPLETO.pdf.jpgimage/jpeg5779http://tede2.pucrs.br/tede2/bitstream/tede/7950/7/DIS_DARLAN_GUSSO_COMPLETO.pdf.jpg7cceb6655180bf5d63baaff4f00b2725MD57TEXTDIS_DARLAN_GUSSO_CONFIDENCIAL.pdf.txtDIS_DARLAN_GUSSO_CONFIDENCIAL.pdf.txttext/plain1151http://tede2.pucrs.br/tede2/bitstream/tede/7950/3/DIS_DARLAN_GUSSO_CONFIDENCIAL.pdf.txtaadb1e3636375337d0afb6e464a4caf2MD53DIS_DARLAN_GUSSO_COMPLETO.pdf.txtDIS_DARLAN_GUSSO_COMPLETO.pdf.txttext/plain104591http://tede2.pucrs.br/tede2/bitstream/tede/7950/6/DIS_DARLAN_GUSSO_COMPLETO.pdf.txte4e5911ce14ffb982f63632decbc74f4MD56LICENSElicense.txtlicense.txttext/plain; charset=utf-8610http://tede2.pucrs.br/tede2/bitstream/tede/7950/1/license.txt5a9d6006225b368ef605ba16b4f6d1beMD51tede/79502020-08-05 20:01:14.032oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2020-08-05T23:01:14Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.por.fl_str_mv Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)
title Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)
spellingShingle Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)
Gusso, Darlan
Comportamento
Memória
Larvicida
Piriproxifeno
Peixe-zebra
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)
title_full Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)
title_fullStr Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)
title_full_unstemmed Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)
title_sort Efeito do Piriproxifeno sobre o desenvolvimento, parâmetros comportamentais e endócrinos em larvas e adultos de Peixe-zebra (Danio rerio)
author Gusso, Darlan
author_facet Gusso, Darlan
author_role author
dc.contributor.advisor1.fl_str_mv Bonan, Carla Denise
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/8058052532279136
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/1278147802553764
dc.contributor.author.fl_str_mv Gusso, Darlan
contributor_str_mv Bonan, Carla Denise
dc.subject.por.fl_str_mv Comportamento
Memória
Larvicida
Piriproxifeno
Peixe-zebra
topic Comportamento
Memória
Larvicida
Piriproxifeno
Peixe-zebra
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description Mosquitoes are responsible for the transmission of various pathogens. Over the past three years, the world has witnessed epidemics affecting all age groups, such as dengue fever, yellow fever, chikungunya and zika virus. To control and eliminate Aedes vectors responsible for the transmission of these diseases, it is necessary to use larvicides and insecticides such as pyriproxyfen. Pyriproxyfen is a larvicide used to control mosquitoes, mainly of the genus Aedes. This study evaluated the effects of pyriproxyfen on survival, morphology, behavior, and endocrine parameters at different stages of zebrafish development. We have demonstrated that pyriproxyfen can cause changes in the survival rate, behavior and morphology of exposed larvae, adults exposed at the larval stage, and adult offspring of exposed parents. Larvae exposed to pyriproxyfen for 1-96 hpf (hours post-fertilization) showed a decrease in survival rate, heart rate 3 dpf (days post-fertilization), and body length at 5 and 8 dpf. The distance traveled and the mean velocities of the exposed larvae were reduced in comparison to the vehicle group (1% DMSO). Therefore, pyriproxyfen changes the morphology and behavior of zebrafish in early stages of development and may affect the next generations. However, when the exposed larvae were maintained until the adult stage (6 months), we did not observe differences in locomotor parameters. Parental exposure to pyriproxyfen induced decrease in locomotion of zebrafish adult offspring as well as an anxiolytic-like behavior. We also tested pyriproxyfen exposure for 96 hours in adult zebrafish and the locomotion, anxiety, memory and endocrine parameters were analyzed as well as the gene expression of the glucocorticoid receptor (GR) and corticotropin-releasing factor (CRF). These results showed that there was no significant difference in locomotion, anxiety and endocrine parameters. In addition, there was an impairment of habituation memory in adult zebrafish exposed to pyriproxyfen. Therefore, it is important to control the use of larvicides due to their toxic effects on non-target species. Our findings demonstrated the importance of studies related to the use of larvicides, since they are potential causes of morphological and behavioral alterations in aquatic species, such as zebrafish.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-04-19T16:43:13Z
dc.date.issued.fl_str_mv 2018-02-21
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