Papel do baço na resposta inflamatória cerebral em modelo experimental de epilepsia em ratos tratados com células mononucleares de medula óssea

• Autor(a) principal: Silva, Daniele Vieira da
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da PUC_RS
• Texto Completo: http://tede2.pucrs.br/tede2/handle/tede/6256
• topic: MEDICINA; PEDIATRIA; EPILEPSIA; BAÇO; CITOCINAS; MEMÓRIA; RATOS - EXPERIÊNCIAS; DOENÇAS DO SISTEMA NERVOSO; CLINICA MEDICA::PEDIATRIA

Epilepsy comprehends a category of syndromes characterized by spontaneous and recurrent crises (SRCs), which are a result of intense electric activity, synchronized and rhythmic of neuronal populations of the central nervous system (CNS), and of excessive excitability in the absence of a toxic-metabolic condition or fever 1 2, 3. Experimental studies have shown that SE induction in rodents provokes pronounced inflammatory cerebral reaction involving endothelial cells, glia, and neurons4, 5. Our group has shown that intravenous bone marrow cells administration following lithium-pilocarpine SE induction reduces SRCs, improves cognitive function, and modulates the production of pro-inflammatory cytokines6, 7. The spleen is important to induce cerebral inflammatory response and also is an important target for stem cell intervention therapies8. Splenectomy before a cerebrovascular accident (CVA) significantly reduces the infarction size in the brain9. Moreover, it has been demonstrated that in the pilocarpine model splenectomy decreases the severity of crises, the time to SE onset, and the mortality rate caused by SE10. The aim of the present work was investigating the importance of the interaction between bone marrow mononuclear cells (BMC) and the spleen in epileptic rats. As a result, the animals treated with BMC presented a better capability of memory retrieval in the Morris aquatic maze (MAM) when compared to the treated animals without the spleen. Besides, the treatment did not present the potential of reducing the levels of IL-1 β or of increasing the levels of IL-10 in the serum and hippocampus of treated rats without the spleen. In conclusion, the work suggests that the spleen could be involved in the therapeutic effect promoted by BMC transplant.