Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal

Detalhes bibliográficos
Autor(a) principal: Faillace, Giulia Ramos
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da PUC_RS
Texto Completo: http://tede2.pucrs.br/tede2/handle/tede/6268
Resumo: The potato is the third most important consumed food in the world. Despite its high productivity, many pest problems cause losses in the quantity and quality of the product. Its high susceptibility to pathogens such as the necrotrophic bacteria Pectobacterium atrosepticum, has led to the indiscriminate use of pesticides, causing damage to the environment and human population. Studies of plant resistance inducers represent an alternative for disease control in some cultures by promoting innate defense system against pathogens. Understanding the changes in plant metabolism related to defense enables the evaluation and development of new strategies and products to disease control. This work aimed was to evaluate some of the mechanisms involved in defense metabolism of Solanum tuberosum in response to the phytobacteria Pectobacterium atrosepticum as well as to the XTH and ASM elicitors.To this end, we analyzed the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APx), enzymes related to defense metabolism, polyphenol oxidase (PPO), peroxidase (POX), phenylalanine ammonia lyase (PAL), and pathogenesis-related proteins, β-1,3-glucanase and chitinase. It was also determined the synthesis of phenolic compounds and salicylic acid (SA), and the assessment of disease progression caused by the phytobacteria in leaves pretreated with XTH. Our results showed that the elicitor XTH was able to delay the disease progression and symptoms caused by the bacteria P. atrosepticum.The elicitors ASM and XTH promoted the activation of the antioxidant enzymes, as well as the induction of certain enzymes related to defense before and after the bacterial inoculation, differing from the response pattern observed in the leaves treated only with P. atrosepticum. In addition, we observed an increase in free SA levels in leaves treated only with the phytobacteria, differing from the other treatments. In conclusion, the elicitor XTH promotes resistance to the necrotrophic bacteria P. atrospeticum by the defense metabolism activation and modulation of S. tuberosum.
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spelling Astarita, Leandro Vieira644.533.830-53021.278.280-03http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4481916P3Faillace, Giulia Ramos2015-08-25T11:42:48Z2015-03-18http://tede2.pucrs.br/tede2/handle/tede/6268The potato is the third most important consumed food in the world. Despite its high productivity, many pest problems cause losses in the quantity and quality of the product. Its high susceptibility to pathogens such as the necrotrophic bacteria Pectobacterium atrosepticum, has led to the indiscriminate use of pesticides, causing damage to the environment and human population. Studies of plant resistance inducers represent an alternative for disease control in some cultures by promoting innate defense system against pathogens. Understanding the changes in plant metabolism related to defense enables the evaluation and development of new strategies and products to disease control. This work aimed was to evaluate some of the mechanisms involved in defense metabolism of Solanum tuberosum in response to the phytobacteria Pectobacterium atrosepticum as well as to the XTH and ASM elicitors.To this end, we analyzed the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APx), enzymes related to defense metabolism, polyphenol oxidase (PPO), peroxidase (POX), phenylalanine ammonia lyase (PAL), and pathogenesis-related proteins, β-1,3-glucanase and chitinase. It was also determined the synthesis of phenolic compounds and salicylic acid (SA), and the assessment of disease progression caused by the phytobacteria in leaves pretreated with XTH. Our results showed that the elicitor XTH was able to delay the disease progression and symptoms caused by the bacteria P. atrosepticum.The elicitors ASM and XTH promoted the activation of the antioxidant enzymes, as well as the induction of certain enzymes related to defense before and after the bacterial inoculation, differing from the response pattern observed in the leaves treated only with P. atrosepticum. In addition, we observed an increase in free SA levels in leaves treated only with the phytobacteria, differing from the other treatments. In conclusion, the elicitor XTH promotes resistance to the necrotrophic bacteria P. atrospeticum by the defense metabolism activation and modulation of S. tuberosum.A batata destaca-se como o terceiro alimento mais consumido no mundo. Apesar de sua alta produtividade, inúmeros problemas fitossanitários vêm causando perdas na quantidade e na qualidade do produto. Sua alta suscetibilidade a patógenos, como a bactéria necrotrófica Pectobacterium atrosepticum, tem levado ao uso indiscriminado de agrotóxicos, causando danos ao meio ambiente e à população humana. Estudos com indutores de resistência vegetal têm representado uma alternativa para o controle de doenças em algumas culturas através da promoção do sistema inato de defesa contra patógenos. Compreender as modificações no metabolismo vegetal relacionado à defesa possibilita a avaliação e o desenvolvimento de novos recursos e produtos para o manejo fitossanitário.Este trabalho teve por objetivo avaliar alguns dos mecanismos envolvidos no metabolismo de defesa de Solanum tuberosum em resposta à fitopabactéria Pectobacterium atrosepticum e aos indutores XTH e ASM. Para tal, foram realizadas análises de atividade das enzimas antioxidantes superóxido dismutase (SOD), catalase (CAT) e ascorbato peroxidase (APx), das enzimas relacionadas ao metabolismo de defesa, polifenoloxidase (PPO), peroxidase (POX), fenilalanina amônia liase (PAL), e das proteínas relacionadas à patôgenese, β-1,3-glucanase e quitinase. Foi determinado também o acúmulo de compostos fenólicos e de ácido salicílico (AS), além da avaliação da manifestação da doença provocada pela fitobactéria em folhas pré-tratadas com XTH. Os resultados demonstraram que o indutor XTH foi capaz de retardar a progressão e os sintomas da doença provocados pela bactéria P. atrosepticum em folhas de batata.Os indutores XTH e ASM promoveram a ativação das enzimas antioxidantes, assim como a indução de enzimas relacionadas à defesa, anteriormente e posteriormente à inoculação de P. atrosepticum, diferindo do padrão de resposta observado nas folhas tratadas apenas com a bactéria patogênica. Além disso, foi observado um aumento dos níveis de AS livre nas folhas tratadas apenas com a fitobactéria, diferindo dos outros tratamentos. A partir destes resultados conclui-se que o indutor XTH promove resistência contra a bactéria necrotrófica P. atrospeticum através da ativação e modulação do metabolismo de defesa em S. tuberosum.Submitted by Setor de Tratamento da Informação - BC/PUCRS (tede2@pucrs.br) on 2015-08-25T11:42:48Z No. of bitstreams: 1 474060 - Texto Completo.pdf: 1372189 bytes, checksum: a43c7ff8c9402604b61dba9bcef89a8f (MD5)Made available in DSpace on 2015-08-25T11:42:48Z (GMT). No. of bitstreams: 1 474060 - Texto Completo.pdf: 1372189 bytes, checksum: a43c7ff8c9402604b61dba9bcef89a8f (MD5) Previous issue date: 2015-03-18application/pdfhttp://tede2.pucrs.br:80/tede2/retrieve/163270/474060%20-%20Texto%20Completo.pdf.jpgporPontifícia Universidade Católica do Rio Grande do SulPrograma de Pós-Graduação em Biologia Celular e MolecularPUCRSBrasilFaculdade de BiociênciasBIOLOGIA CELULARBIOLOGIA MOLECULARBATATASENZIMASPLANTAS - DOENÇASCIENCIAS BIOLOGICAS::BIOLOGIA GERALMetabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis819824693009663736060060060036528317262667714-1634559385931244697info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da PUC_RSinstname:Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)instacron:PUC_RSTHUMBNAIL474060 - Texto Completo.pdf.jpg474060 - Texto Completo.pdf.jpgimage/jpeg3177http://tede2.pucrs.br/tede2/bitstream/tede/6268/4/474060+-+Texto+Completo.pdf.jpg38f1186466ae4da2722d9a69ebdebe44MD54TEXT474060 - Texto Completo.pdf.txt474060 - Texto Completo.pdf.txttext/plain169248http://tede2.pucrs.br/tede2/bitstream/tede/6268/3/474060+-+Texto+Completo.pdf.txt7ef1841ea76ec5762f00664eb92b1ce0MD53ORIGINAL474060 - Texto Completo.pdf474060 - Texto Completo.pdfapplication/pdf1372189http://tede2.pucrs.br/tede2/bitstream/tede/6268/2/474060+-+Texto+Completo.pdfa43c7ff8c9402604b61dba9bcef89a8fMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-8610http://tede2.pucrs.br/tede2/bitstream/tede/6268/1/license.txt5a9d6006225b368ef605ba16b4f6d1beMD51tede/62682015-09-29 08:31:49.507oai:tede2.pucrs.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://tede2.pucrs.br/tede2/PRIhttps://tede2.pucrs.br/oai/requestbiblioteca.central@pucrs.br||opendoar:2015-09-29T11:31:49Biblioteca Digital de Teses e Dissertações da PUC_RS - Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS)false
dc.title.por.fl_str_mv Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal
title Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal
spellingShingle Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal
Faillace, Giulia Ramos
BIOLOGIA CELULAR
BIOLOGIA MOLECULAR
BATATAS
ENZIMAS
PLANTAS - DOENÇAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
title_short Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal
title_full Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal
title_fullStr Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal
title_full_unstemmed Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal
title_sort Metabolismo de defesa em Solanum tuberosum em resposta à fitobactéria Pectobacterium atrosepticum e a indutores de resistência vegetal
author Faillace, Giulia Ramos
author_facet Faillace, Giulia Ramos
author_role author
dc.contributor.advisor1.fl_str_mv Astarita, Leandro Vieira
dc.contributor.advisor1ID.fl_str_mv 644.533.830-53
dc.contributor.authorID.fl_str_mv 021.278.280-03
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4481916P3
dc.contributor.author.fl_str_mv Faillace, Giulia Ramos
contributor_str_mv Astarita, Leandro Vieira
dc.subject.por.fl_str_mv BIOLOGIA CELULAR
BIOLOGIA MOLECULAR
BATATAS
ENZIMAS
PLANTAS - DOENÇAS
topic BIOLOGIA CELULAR
BIOLOGIA MOLECULAR
BATATAS
ENZIMAS
PLANTAS - DOENÇAS
CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
dc.subject.cnpq.fl_str_mv CIENCIAS BIOLOGICAS::BIOLOGIA GERAL
description The potato is the third most important consumed food in the world. Despite its high productivity, many pest problems cause losses in the quantity and quality of the product. Its high susceptibility to pathogens such as the necrotrophic bacteria Pectobacterium atrosepticum, has led to the indiscriminate use of pesticides, causing damage to the environment and human population. Studies of plant resistance inducers represent an alternative for disease control in some cultures by promoting innate defense system against pathogens. Understanding the changes in plant metabolism related to defense enables the evaluation and development of new strategies and products to disease control. This work aimed was to evaluate some of the mechanisms involved in defense metabolism of Solanum tuberosum in response to the phytobacteria Pectobacterium atrosepticum as well as to the XTH and ASM elicitors.To this end, we analyzed the activities of antioxidant enzymes superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APx), enzymes related to defense metabolism, polyphenol oxidase (PPO), peroxidase (POX), phenylalanine ammonia lyase (PAL), and pathogenesis-related proteins, β-1,3-glucanase and chitinase. It was also determined the synthesis of phenolic compounds and salicylic acid (SA), and the assessment of disease progression caused by the phytobacteria in leaves pretreated with XTH. Our results showed that the elicitor XTH was able to delay the disease progression and symptoms caused by the bacteria P. atrosepticum.The elicitors ASM and XTH promoted the activation of the antioxidant enzymes, as well as the induction of certain enzymes related to defense before and after the bacterial inoculation, differing from the response pattern observed in the leaves treated only with P. atrosepticum. In addition, we observed an increase in free SA levels in leaves treated only with the phytobacteria, differing from the other treatments. In conclusion, the elicitor XTH promotes resistance to the necrotrophic bacteria P. atrospeticum by the defense metabolism activation and modulation of S. tuberosum.
publishDate 2015
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