Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers

Detalhes bibliográficos
Autor(a) principal: Venâncio, Armando
Data de Publicação: 1996
Outros Autores: Almeida, Catarina, Teixeira, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/3699
Resumo: The main drawback when using aqueous two-phase systems for macromolecule purification is the high cost of most polymers used. The purification of an enzyme, alcohol dehydrogenase, from a crude extract of Saccharomyces cerevisiae was tested in systems composed of poly(ethylene glycol) and a crude hydroxypropyl starch or Reppal PES 100, a purified fraction of hydroxypropyl starch. Purification factors measured for the enzyme were very similar in both systems (between 0.8 and 1.4 for both systems in the upper phase). However, systems composed of Reppal PES present a greater recovery of enzyme, between 77% and 100% versus 60% and 100%, while systems composed of crude hydroxypropyl starch exhibit a larger Δlog K for the tested ligand, 1.26 versus 0.81.
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spelling Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymersEnzymeAlcohol dehydrogenasePolymerScience & TechnologyThe main drawback when using aqueous two-phase systems for macromolecule purification is the high cost of most polymers used. The purification of an enzyme, alcohol dehydrogenase, from a crude extract of Saccharomyces cerevisiae was tested in systems composed of poly(ethylene glycol) and a crude hydroxypropyl starch or Reppal PES 100, a purified fraction of hydroxypropyl starch. Purification factors measured for the enzyme were very similar in both systems (between 0.8 and 1.4 for both systems in the upper phase). However, systems composed of Reppal PES present a greater recovery of enzyme, between 77% and 100% versus 60% and 100%, while systems composed of crude hydroxypropyl starch exhibit a larger Δlog K for the tested ligand, 1.26 versus 0.81.Junta Nacional de Investigação Científica e Tecnológica (JNICT).Elsevier ScienceUniversidade do MinhoVenâncio, ArmandoAlmeida, CatarinaTeixeira, J. A.1996-05-171996-05-17T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/3699eng"Journal of Chromatography, B : Biomedical Applications". ISSN 0378-4347. 680:1/2 (1996) 131-136.0378-434710.1016/0378-4347(95)00419-X8798890info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:54:18Zoai:repositorium.sdum.uminho.pt:1822/3699Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:48.919999Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers
title Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers
spellingShingle Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers
Venâncio, Armando
Enzyme
Alcohol dehydrogenase
Polymer
Science & Technology
title_short Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers
title_full Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers
title_fullStr Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers
title_full_unstemmed Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers
title_sort Enzyme purification with aqueous two-phase systems: comparison between systems composed of pure polymers and systems composed of crude polymers
author Venâncio, Armando
author_facet Venâncio, Armando
Almeida, Catarina
Teixeira, J. A.
author_role author
author2 Almeida, Catarina
Teixeira, J. A.
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Venâncio, Armando
Almeida, Catarina
Teixeira, J. A.
dc.subject.por.fl_str_mv Enzyme
Alcohol dehydrogenase
Polymer
Science & Technology
topic Enzyme
Alcohol dehydrogenase
Polymer
Science & Technology
description The main drawback when using aqueous two-phase systems for macromolecule purification is the high cost of most polymers used. The purification of an enzyme, alcohol dehydrogenase, from a crude extract of Saccharomyces cerevisiae was tested in systems composed of poly(ethylene glycol) and a crude hydroxypropyl starch or Reppal PES 100, a purified fraction of hydroxypropyl starch. Purification factors measured for the enzyme were very similar in both systems (between 0.8 and 1.4 for both systems in the upper phase). However, systems composed of Reppal PES present a greater recovery of enzyme, between 77% and 100% versus 60% and 100%, while systems composed of crude hydroxypropyl starch exhibit a larger Δlog K for the tested ligand, 1.26 versus 0.81.
publishDate 1996
dc.date.none.fl_str_mv 1996-05-17
1996-05-17T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/3699
url http://hdl.handle.net/1822/3699
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Journal of Chromatography, B : Biomedical Applications". ISSN 0378-4347. 680:1/2 (1996) 131-136.
0378-4347
10.1016/0378-4347(95)00419-X
8798890
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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