A new marker for rapid sex identification of red deer (Cervus elaphus)

Detalhes bibliográficos
Autor(a) principal: Barbosa, A. Márcia
Data de Publicação: 2009
Outros Autores: Fernández-García, José Luís, Carranza, Juan
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/2050
Resumo: The red deer (Cervus elaphus) is an economically important game species facing some conservation issues. The knowledge of population parameters such as sex ratios is an important basis for management and conservation. We used previously published Y-chromosomal primers under low-stringency amplification conditions and found that they allow quick and accurate sex identification in this species by producing two different amplicons in males and one in females. Both fragments are clearly different in size and easily separated through agarose gel electrophoresis, allowing one-step sex identification in biological samples such as carcasses, embryos recovered from culled females, and possibly hair or excrements (given sufficient DNA quality). This can help studies on intrauterine sex ratio, sex-biased mortality, or the sex ratio of wild or extensive game populations, with useful applications in demographic studies, game management, and conservation.
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spelling A new marker for rapid sex identification of red deer (Cervus elaphus)Cervus elaphusgender identificationsex markerY chromosomegame managementThe red deer (Cervus elaphus) is an economically important game species facing some conservation issues. The knowledge of population parameters such as sex ratios is an important basis for management and conservation. We used previously published Y-chromosomal primers under low-stringency amplification conditions and found that they allow quick and accurate sex identification in this species by producing two different amplicons in males and one in females. Both fragments are clearly different in size and easily separated through agarose gel electrophoresis, allowing one-step sex identification in biological samples such as carcasses, embryos recovered from culled females, and possibly hair or excrements (given sufficient DNA quality). This can help studies on intrauterine sex ratio, sex-biased mortality, or the sex ratio of wild or extensive game populations, with useful applications in demographic studies, game management, and conservation.Associazione Teriologica Italiana2010-09-15T08:28:33Z2010-09-152009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article60261 bytesapplication/pdfhttp://hdl.handle.net/10174/2050http://hdl.handle.net/10174/2050eng169-17220Hystrix - Italian Journal of Mammalogy20livrebarbosa@uevora.ptpepelufe@unex.escarranza@unex.es552Barbosa, A. MárciaFernández-García, José LuísCarranza, Juaninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T18:37:58Zoai:dspace.uevora.pt:10174/2050Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:57:45.056847Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A new marker for rapid sex identification of red deer (Cervus elaphus)
title A new marker for rapid sex identification of red deer (Cervus elaphus)
spellingShingle A new marker for rapid sex identification of red deer (Cervus elaphus)
Barbosa, A. Márcia
Cervus elaphus
gender identification
sex marker
Y chromosome
game management
title_short A new marker for rapid sex identification of red deer (Cervus elaphus)
title_full A new marker for rapid sex identification of red deer (Cervus elaphus)
title_fullStr A new marker for rapid sex identification of red deer (Cervus elaphus)
title_full_unstemmed A new marker for rapid sex identification of red deer (Cervus elaphus)
title_sort A new marker for rapid sex identification of red deer (Cervus elaphus)
author Barbosa, A. Márcia
author_facet Barbosa, A. Márcia
Fernández-García, José Luís
Carranza, Juan
author_role author
author2 Fernández-García, José Luís
Carranza, Juan
author2_role author
author
dc.contributor.author.fl_str_mv Barbosa, A. Márcia
Fernández-García, José Luís
Carranza, Juan
dc.subject.por.fl_str_mv Cervus elaphus
gender identification
sex marker
Y chromosome
game management
topic Cervus elaphus
gender identification
sex marker
Y chromosome
game management
description The red deer (Cervus elaphus) is an economically important game species facing some conservation issues. The knowledge of population parameters such as sex ratios is an important basis for management and conservation. We used previously published Y-chromosomal primers under low-stringency amplification conditions and found that they allow quick and accurate sex identification in this species by producing two different amplicons in males and one in females. Both fragments are clearly different in size and easily separated through agarose gel electrophoresis, allowing one-step sex identification in biological samples such as carcasses, embryos recovered from culled females, and possibly hair or excrements (given sufficient DNA quality). This can help studies on intrauterine sex ratio, sex-biased mortality, or the sex ratio of wild or extensive game populations, with useful applications in demographic studies, game management, and conservation.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01T00:00:00Z
2010-09-15T08:28:33Z
2010-09-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/2050
http://hdl.handle.net/10174/2050
url http://hdl.handle.net/10174/2050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 169-172
20
Hystrix - Italian Journal of Mammalogy
20
livre
barbosa@uevora.pt
pepelufe@unex.es
carranza@unex.es
552
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 60261 bytes
application/pdf
dc.publisher.none.fl_str_mv Associazione Teriologica Italiana
publisher.none.fl_str_mv Associazione Teriologica Italiana
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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