Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues

Detalhes bibliográficos
Autor(a) principal: Moreira, Helena R.
Data de Publicação: 2023
Outros Autores: Cerqueira, Mariana T., Silva, Lucília Pereira, Pires, Joana, Jarnalo, Mariana, Horta, Ricardo, Reis, R. L., Marques, A. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/86674
Resumo: Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2bm02022j
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spelling Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analoguesIn vitro modelsPapillary fibroblastsReticular fibroblastsSkinVascularizationElectronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2bm02022jThe papillary and reticular dermis harbors phenotypically distinct fibroblasts, whose functions such as maintenance of skin's microvasculature are also distinct. Thus, we hypothesized that pre-selection of the subpopulations of fibroblasts would benefit the generation of skin tissue engineered (TE) constructs, promoting their prevascularization in vitro. We first isolated papillary and reticular fibroblasts using fluorescence-activated cell sorting and studied the effect of their secretome and extracellular matrix (ECM) on human dermal microvascular endothelial cell (hDMEC) organization. Subsequently, we developed a bilayered 3D polymeric structure with distinct layer-associated features to house the subpopulations of fibroblasts, to generate a skin analogue. Both papillary and reticular fibroblasts were able to stimulate capillary-like network formation in a Matrigel assay. However, the secretome of the two subpopulations was substantially different, being enriched in VEGF, IGF-1, and Angio-1 in the case of papillary fibroblasts and in HGF and FGF-2 for the reticular subset. In addition, the fibroblast subpopulations deposited varied levels of ECM proteins, more collagen I and laminin was produced by the reticular subset, but these differences did not impact hDMEC organization. Vessel-like structures with lumens were observed earlier in the 3D skin analogue prepared with the sorted fibroblasts, although ECM deposition was not affected by the cell's pre-selection. Moreover, a more differentiated epidermal layer was obtained in the skin analogue formed by the sorted fibroblasts, confirming that its whole structure was not affected. Overall, we provide evidence that pre-selection of papillary and reticular fibroblasts is relevant for promoting the in vitro prevascularization of skin TE constructs.The authors would like to acknowledge the financial support from the Consolidator Grant “ECM_INK” (ERC-2016- COG-726061), to the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the PD/169/2013, NORTE-08-5369-FSE-000037 (H.R. M.).Royal Society of ChemistryUniversidade do MinhoMoreira, Helena R.Cerqueira, Mariana T.Silva, Lucília PereiraPires, JoanaJarnalo, MarianaHorta, RicardoReis, R. L.Marques, A. P.2023-052023-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/86674engMoreira H. R., Cerqueira M. T., da Silva L. P., Pires J., Jarnalo M., Horta R., Reis R. L., Marques A. P. Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues, Biomaterials Science, Vol. 11, pp. 5287, doi:10.1039/D2BM02022J, 20232047-48302047-484910.1039/D2BM02022J37350513https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm02022jinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-30T01:29:16Zoai:repositorium.sdum.uminho.pt:1822/86674Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:33:32.011377Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues
title Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues
spellingShingle Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues
Moreira, Helena R.
In vitro models
Papillary fibroblasts
Reticular fibroblasts
Skin
Vascularization
title_short Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues
title_full Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues
title_fullStr Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues
title_full_unstemmed Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues
title_sort Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues
author Moreira, Helena R.
author_facet Moreira, Helena R.
Cerqueira, Mariana T.
Silva, Lucília Pereira
Pires, Joana
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Marques, A. P.
author_role author
author2 Cerqueira, Mariana T.
Silva, Lucília Pereira
Pires, Joana
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Marques, A. P.
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Moreira, Helena R.
Cerqueira, Mariana T.
Silva, Lucília Pereira
Pires, Joana
Jarnalo, Mariana
Horta, Ricardo
Reis, R. L.
Marques, A. P.
dc.subject.por.fl_str_mv In vitro models
Papillary fibroblasts
Reticular fibroblasts
Skin
Vascularization
topic In vitro models
Papillary fibroblasts
Reticular fibroblasts
Skin
Vascularization
description Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2bm02022j
publishDate 2023
dc.date.none.fl_str_mv 2023-05
2023-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/86674
url https://hdl.handle.net/1822/86674
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Moreira H. R., Cerqueira M. T., da Silva L. P., Pires J., Jarnalo M., Horta R., Reis R. L., Marques A. P. Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues, Biomaterials Science, Vol. 11, pp. 5287, doi:10.1039/D2BM02022J, 2023
2047-4830
2047-4849
10.1039/D2BM02022J
37350513
https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm02022j
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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