Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/27085 |
Resumo: | Aerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein: starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO2 sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications. |
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Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approachStarch aerogelsZeinSupercritical sterilizationSupercritical CO2Regenerative medicineAerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein: starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO2 sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications.MDPIVeritati - Repositório Institucional da Universidade Católica PortuguesaSantos-Rosales, VíctorArdao, InésAlvarez-Lorenzo, CarmenRibeiro, NilzaOliveira, Ana L.García-González, Carlos A.2019-03-13T15:07:27Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/27085engSantos-Rosales, V., Ardao, I., Alvarez-Lorenzo, C., Ribeiro, N., Oliveira, A., & García-González, C. (2019). Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO2-Based Approach. Molecules, 24(5), 871. https://doi.org/10.3390/molecules240508711420-304910.3390/molecules2405087185062889223PMC642919430823685000462662900047info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-03T01:41:43Zoai:repositorio.ucp.pt:10400.14/27085Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:21:39.566430Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach |
title |
Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach |
spellingShingle |
Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach Santos-Rosales, Víctor Starch aerogels Zein Supercritical sterilization Supercritical CO2 Regenerative medicine |
title_short |
Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach |
title_full |
Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach |
title_fullStr |
Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach |
title_full_unstemmed |
Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach |
title_sort |
Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach |
author |
Santos-Rosales, Víctor |
author_facet |
Santos-Rosales, Víctor Ardao, Inés Alvarez-Lorenzo, Carmen Ribeiro, Nilza Oliveira, Ana L. García-González, Carlos A. |
author_role |
author |
author2 |
Ardao, Inés Alvarez-Lorenzo, Carmen Ribeiro, Nilza Oliveira, Ana L. García-González, Carlos A. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Santos-Rosales, Víctor Ardao, Inés Alvarez-Lorenzo, Carmen Ribeiro, Nilza Oliveira, Ana L. García-González, Carlos A. |
dc.subject.por.fl_str_mv |
Starch aerogels Zein Supercritical sterilization Supercritical CO2 Regenerative medicine |
topic |
Starch aerogels Zein Supercritical sterilization Supercritical CO2 Regenerative medicine |
description |
Aerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein: starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO2 sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-03-13T15:07:27Z 2019 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/27085 |
url |
http://hdl.handle.net/10400.14/27085 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos-Rosales, V., Ardao, I., Alvarez-Lorenzo, C., Ribeiro, N., Oliveira, A., & García-González, C. (2019). Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO2-Based Approach. Molecules, 24(5), 871. https://doi.org/10.3390/molecules24050871 1420-3049 10.3390/molecules24050871 85062889223 PMC6429194 30823685 000462662900047 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799131917753253888 |