Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach

Detalhes bibliográficos
Autor(a) principal: Santos-Rosales, Víctor
Data de Publicação: 2019
Outros Autores: Ardao, Inés, Alvarez-Lorenzo, Carmen, Ribeiro, Nilza, Oliveira, Ana L., García-González, Carlos A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/27085
Resumo: Aerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein: starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO2 sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications.
id RCAP_0086a4d450a020e076718aa11ee744f4
oai_identifier_str oai:repositorio.ucp.pt:10400.14/27085
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approachStarch aerogelsZeinSupercritical sterilizationSupercritical CO2Regenerative medicineAerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein: starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO2 sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications.MDPIVeritati - Repositório Institucional da Universidade Católica PortuguesaSantos-Rosales, VíctorArdao, InésAlvarez-Lorenzo, CarmenRibeiro, NilzaOliveira, Ana L.García-González, Carlos A.2019-03-13T15:07:27Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/27085engSantos-Rosales, V., Ardao, I., Alvarez-Lorenzo, C., Ribeiro, N., Oliveira, A., & García-González, C. (2019). Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO2-Based Approach. Molecules, 24(5), 871. https://doi.org/10.3390/molecules240508711420-304910.3390/molecules2405087185062889223PMC642919430823685000462662900047info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-10-03T01:41:43Zoai:repositorio.ucp.pt:10400.14/27085Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:21:39.566430Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
title Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
spellingShingle Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
Santos-Rosales, Víctor
Starch aerogels
Zein
Supercritical sterilization
Supercritical CO2
Regenerative medicine
title_short Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
title_full Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
title_fullStr Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
title_full_unstemmed Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
title_sort Sterile and dual-porous aerogels scaffolds obtained through a multistep supercritical CO2-based approach
author Santos-Rosales, Víctor
author_facet Santos-Rosales, Víctor
Ardao, Inés
Alvarez-Lorenzo, Carmen
Ribeiro, Nilza
Oliveira, Ana L.
García-González, Carlos A.
author_role author
author2 Ardao, Inés
Alvarez-Lorenzo, Carmen
Ribeiro, Nilza
Oliveira, Ana L.
García-González, Carlos A.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Santos-Rosales, Víctor
Ardao, Inés
Alvarez-Lorenzo, Carmen
Ribeiro, Nilza
Oliveira, Ana L.
García-González, Carlos A.
dc.subject.por.fl_str_mv Starch aerogels
Zein
Supercritical sterilization
Supercritical CO2
Regenerative medicine
topic Starch aerogels
Zein
Supercritical sterilization
Supercritical CO2
Regenerative medicine
description Aerogels from natural polymers are endowed with attractive textural and biological properties for biomedical applications due to their high open mesoporosity, low density, and reduced toxicity. Nevertheless, the lack of macroporosity in the aerogel structure and of a sterilization method suitable for these materials restrict their use for regenerative medicine purposes and prompt the research on getting ready-to-implant dual (macro + meso)porous aerogels. In this work, zein, a family of proteins present in materials for tissue engineering, was evaluated as a sacrificial porogen to obtain macroporous starch aerogels. This approach was particularly advantageous since it could be integrated in the conventional aerogel processing method without extra leaching steps. Physicochemical, morphological, and mechanical characterization were performed to study the effect of porogen zein at various proportions (0:1, 1:2, and 1:1 zein: starch weight ratio) on the properties of the obtained starch-based aerogels. From a forward-looking perspective for its clinical application, a supercritical CO2 sterilization treatment was implemented for these aerogels. The sterilization efficacy and the influence of the treatment on the aerogel final properties were evaluated mainly in terms of absence of microbial growth, cytocompatibility, as well as physicochemical, structural, and mechanical modifications.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-13T15:07:27Z
2019
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/27085
url http://hdl.handle.net/10400.14/27085
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Santos-Rosales, V., Ardao, I., Alvarez-Lorenzo, C., Ribeiro, N., Oliveira, A., & García-González, C. (2019). Sterile and Dual-Porous Aerogels Scaffolds Obtained through a Multistep Supercritical CO2-Based Approach. Molecules, 24(5), 871. https://doi.org/10.3390/molecules24050871
1420-3049
10.3390/molecules24050871
85062889223
PMC6429194
30823685
000462662900047
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131917753253888