PrP(106-126) does not interact with membranes under physiological conditions
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/7260 |
Resumo: | © 2008 by the Biophysical Society |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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PrP(106-126) does not interact with membranes under physiological conditions© 2008 by the Biophysical SocietyTransmissible spongiform encephalopathies are neurodegenerative diseases characterized by the accumulation of an abnormal isoform of the prion protein PrPSc. Its fragment 106-126 has been reported to maintain most of the pathological features of PrPSc, and a role in neurodegeneration has been proposed based on the modulation of membrane properties and channel formation. The ability of PrPSc to modulate membranes and/or form channels in membranes has not been clearly demonstrated; however, if these processes are important, peptide-membrane interactions would be a key feature in the toxicity of PrPSc. In this work, the interaction of PrP(106-126) with model membranes comprising typical lipid identities, as well as more specialized lipids such as phosphatidylserine and GM1 ganglioside, was examined using surface plasmon resonance and fluorescence methodologies. This comprehensive study examines different parameters relevant to characterization of peptidemembrane interactions, including membrane charge, viscosity, lipid composition, pH, and ionic strength. We report that PrP(106-126) has a low affinity for lipid membranes under physiological conditions without evidence of membrane disturbances. Membrane insertion and leakage occur only under conditions in which strong electrostatic interactions operate. These results support the hypothesis that the physiological prion protein PrPC mediates PrP(106-126) toxic effects in neuronal cells.This work was supported by a grant from Fundação para a Ciência e Tecnologia (SFRH/BD/14337/2003 to S.T.H.). The International Union of Biochemistry and Molecular Biology is acknowledged for financial support to S.T.H. for a short-term visit to the Marie-Isabel Aguilar laboratory at Monash University, Victoria, Australia. The support of the Australian Research Council and the Potter Foundation is gratefully acknowledged.Biophysical SocietyRepositório da Universidade de LisboaHenriques, Sónia TroeiraPattenden, Leonard KeithAguilar, Marie-IsabelCastanho, Miguel A. R. B.2012-11-21T15:12:57Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/7260engBiophysical Journal 95(4) 1877–1889, August 20080006-3495hhtp://dx.doi.org/10.1529/biophysj.108.131458info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:50:17Zoai:repositorio.ul.pt:10451/7260Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:32:06.903642Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
PrP(106-126) does not interact with membranes under physiological conditions |
title |
PrP(106-126) does not interact with membranes under physiological conditions |
spellingShingle |
PrP(106-126) does not interact with membranes under physiological conditions Henriques, Sónia Troeira |
title_short |
PrP(106-126) does not interact with membranes under physiological conditions |
title_full |
PrP(106-126) does not interact with membranes under physiological conditions |
title_fullStr |
PrP(106-126) does not interact with membranes under physiological conditions |
title_full_unstemmed |
PrP(106-126) does not interact with membranes under physiological conditions |
title_sort |
PrP(106-126) does not interact with membranes under physiological conditions |
author |
Henriques, Sónia Troeira |
author_facet |
Henriques, Sónia Troeira Pattenden, Leonard Keith Aguilar, Marie-Isabel Castanho, Miguel A. R. B. |
author_role |
author |
author2 |
Pattenden, Leonard Keith Aguilar, Marie-Isabel Castanho, Miguel A. R. B. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Henriques, Sónia Troeira Pattenden, Leonard Keith Aguilar, Marie-Isabel Castanho, Miguel A. R. B. |
description |
© 2008 by the Biophysical Society |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008 2008-01-01T00:00:00Z 2012-11-21T15:12:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/7260 |
url |
http://hdl.handle.net/10451/7260 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biophysical Journal 95(4) 1877–1889, August 2008 0006-3495 hhtp://dx.doi.org/10.1529/biophysj.108.131458 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Biophysical Society |
publisher.none.fl_str_mv |
Biophysical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799134212162322432 |