Applications and perspectives of multi-parameter flow cytometry to microbial biofuels production processes 

• Autor(a) principal: Silva, Teresa Lopes da
• Data de Publicação: 2012
• Outros Autores: Roseiro, J. Carlos, Reis, Alberto
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10400.9/2887
• topic: Biodiesel; Bioethanol; Biomethane; Biohydrogen; Microbial fuel cells; Bioprocess monitoring

Conventional microbiology methods used to monitor microbial biofuels production are based on off-line analyses. The analyses are, unfortunately, insufficient for bioprocess optimization. Real time process control strategies, such as flow cytometry (FC), can be used to monitor bioprocess development (at-line) by providing single cell information that improves process model formulation and validation. This paper reviews the current uses and potential applications of FC in biodiesel, bioethanol, biomethane, biohydrogen and fuel cell processes. By highlighting the inherent accuracy and robustness of the technique for a range of biofuel processing parameters, more robust monitoring and control may be implemented to enhance process efficiency.