Chicken feather keratin peptides for the control of keratinocyte migration

Detalhes bibliográficos
Autor(a) principal: Botelho, Cláudia M.
Data de Publicação: 2021
Outros Autores: Ferreira-Santos, P., Toubarro, Duarte, Dinis, H., Osório, Hugo, Costa-Barbosa, Augusto, Sampaio, Paula, Simões, Nelson, Teixeira, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/73776
Resumo: FAO estimates that in 2030 the poultry meat production could reach 120 million tons, which is a challenge in terms of waste management. Feathers are mainly composed of keratin, an important biomaterial. Using feathers as a source of keratin will minimize the waste generated, while contributing to supply an important material for several industries, such as pharmaceutical and biomedical. The peptides were extracted from the feathers by microbial degradation. In this study, we evaluated the peptides effect on keratinocyte metabolic activity and migration. The influence of these peptides on non-activated and activated macrophages was also assessed. It was demonstrated that depending on the keratin peptide fraction in contact with keratinocytes, it is possible to modulate the migration rate of the keratinocytes. Peptide fraction with low molecular weight increases migration, while peptides with a high range of molecular sizes decreases it. Some peptide fractions induce the secretion of TNF- in non-activated macrophages and not on activated macrophages, demonstrating that these peptides should only be placed in contact with cells, in the context of an ongoing inflammatory process. This work is a step forward on the understanding of keratin peptides influence on keratinocytes and immune cells system cells, macrophages.
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spelling Chicken feather keratin peptides for the control of keratinocyte migrationChicken feathersMicrobial hydrolysisKeratin peptidesKeratinocytesCellular migrationInflammationScience & TechnologyFAO estimates that in 2030 the poultry meat production could reach 120 million tons, which is a challenge in terms of waste management. Feathers are mainly composed of keratin, an important biomaterial. Using feathers as a source of keratin will minimize the waste generated, while contributing to supply an important material for several industries, such as pharmaceutical and biomedical. The peptides were extracted from the feathers by microbial degradation. In this study, we evaluated the peptides effect on keratinocyte metabolic activity and migration. The influence of these peptides on non-activated and activated macrophages was also assessed. It was demonstrated that depending on the keratin peptide fraction in contact with keratinocytes, it is possible to modulate the migration rate of the keratinocytes. Peptide fraction with low molecular weight increases migration, while peptides with a high range of molecular sizes decreases it. Some peptide fractions induce the secretion of TNF- in non-activated macrophages and not on activated macrophages, demonstrating that these peptides should only be placed in contact with cells, in the context of an ongoing inflammatory process. This work is a step forward on the understanding of keratin peptides influence on keratinocytes and immune cells system cells, macrophages.This research was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. It was also was financed by the Portuguese Mass Spectrometry Network, integrated in the National Roadmap of Research Infrastructures of Strategic Relevance (ROTEIRO/0028/2013; LISBOA-01-0145-FEDER-022125) and the MAC 2014-2020-MAC/1.1b/042-INTERREG V A España-Portugal, “BIOTRANSFER 2: Transferencia de la investigación biotecnológica orientada a rentabilidad empresarial y movilización de flujos de negocio 2”.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoBotelho, Cláudia M.Ferreira-Santos, P.Toubarro, DuarteDinis, H.Osório, HugoCosta-Barbosa, AugustoSampaio, PaulaSimões, NelsonTeixeira, J. A.2021-07-232021-07-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73776engBotelho, Cláudia M.; Ferreira-Santos, P.; Toubarro, Duarte; Dinis, H.; Osório, Hugo; Costa-Barbosa, Augusto; Sampaio, Paula; Simões, Nelson; Teixeira, José A., Chicken feather keratin peptides for the control of keratinocyte migration. Applied Sciences, 11(15), 6779, 20212076-341710.3390/app11156779https://www.mdpi.com/2076-3417/11/15/6779info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T06:13:24Zoai:repositorium.sdum.uminho.pt:1822/73776Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T06:13:24Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chicken feather keratin peptides for the control of keratinocyte migration
title Chicken feather keratin peptides for the control of keratinocyte migration
spellingShingle Chicken feather keratin peptides for the control of keratinocyte migration
Botelho, Cláudia M.
Chicken feathers
Microbial hydrolysis
Keratin peptides
Keratinocytes
Cellular migration
Inflammation
Science & Technology
title_short Chicken feather keratin peptides for the control of keratinocyte migration
title_full Chicken feather keratin peptides for the control of keratinocyte migration
title_fullStr Chicken feather keratin peptides for the control of keratinocyte migration
title_full_unstemmed Chicken feather keratin peptides for the control of keratinocyte migration
title_sort Chicken feather keratin peptides for the control of keratinocyte migration
author Botelho, Cláudia M.
author_facet Botelho, Cláudia M.
Ferreira-Santos, P.
Toubarro, Duarte
Dinis, H.
Osório, Hugo
Costa-Barbosa, Augusto
Sampaio, Paula
Simões, Nelson
Teixeira, J. A.
author_role author
author2 Ferreira-Santos, P.
Toubarro, Duarte
Dinis, H.
Osório, Hugo
Costa-Barbosa, Augusto
Sampaio, Paula
Simões, Nelson
Teixeira, J. A.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Botelho, Cláudia M.
Ferreira-Santos, P.
Toubarro, Duarte
Dinis, H.
Osório, Hugo
Costa-Barbosa, Augusto
Sampaio, Paula
Simões, Nelson
Teixeira, J. A.
dc.subject.por.fl_str_mv Chicken feathers
Microbial hydrolysis
Keratin peptides
Keratinocytes
Cellular migration
Inflammation
Science & Technology
topic Chicken feathers
Microbial hydrolysis
Keratin peptides
Keratinocytes
Cellular migration
Inflammation
Science & Technology
description FAO estimates that in 2030 the poultry meat production could reach 120 million tons, which is a challenge in terms of waste management. Feathers are mainly composed of keratin, an important biomaterial. Using feathers as a source of keratin will minimize the waste generated, while contributing to supply an important material for several industries, such as pharmaceutical and biomedical. The peptides were extracted from the feathers by microbial degradation. In this study, we evaluated the peptides effect on keratinocyte metabolic activity and migration. The influence of these peptides on non-activated and activated macrophages was also assessed. It was demonstrated that depending on the keratin peptide fraction in contact with keratinocytes, it is possible to modulate the migration rate of the keratinocytes. Peptide fraction with low molecular weight increases migration, while peptides with a high range of molecular sizes decreases it. Some peptide fractions induce the secretion of TNF- in non-activated macrophages and not on activated macrophages, demonstrating that these peptides should only be placed in contact with cells, in the context of an ongoing inflammatory process. This work is a step forward on the understanding of keratin peptides influence on keratinocytes and immune cells system cells, macrophages.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-23
2021-07-23T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/73776
url http://hdl.handle.net/1822/73776
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Botelho, Cláudia M.; Ferreira-Santos, P.; Toubarro, Duarte; Dinis, H.; Osório, Hugo; Costa-Barbosa, Augusto; Sampaio, Paula; Simões, Nelson; Teixeira, José A., Chicken feather keratin peptides for the control of keratinocyte migration. Applied Sciences, 11(15), 6779, 2021
2076-3417
10.3390/app11156779
https://www.mdpi.com/2076-3417/11/15/6779
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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