Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis

Detalhes bibliográficos
Autor(a) principal: Duarte, José Manuel Martins Abrantes Teixeira
Data de Publicação: 2008
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/540
Resumo: Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
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spelling Botrytis Cinerea mating types distribution and NEP1 protein sequence analysisBotrytis cinereagenes MATproteinas NEPsilenciamento genéticoMAT genesNEP proteinsgene knockoutMestrado em Engenharia Agronómica - Instituto Superior de AgronomiaBotrytis cinerea [teleomorph: Botryotinia fuckeliana] is a heterothallic filamentous plant pathogenic Ascomycete with over two hundred different hosts, and its control at the crop level is still very difficult to achieve. Sexual reproduction as an active part in the fungus dispersal and variability has been underestimated until recent years. Research leading to more insight into the MAT locus and the fundamental principles of the mating process can prompt new strategies for B. cinerea control. During the B. cinerea infection process, phytotoxic proteins such as NEP1 are produced. Their function is yet to be unraveled, but it was postulated that they might play a role in speciation in the genus Botrytis. This report describes a mating type screening of a population of 80 field isolates of B. cinerea. Findings indicate a typical 1:1 Mendelian distribution of the MAT loci, and one strain with both MAT genes is reported. A scan of the MAT locus suggests the absence of the MAT1-1 locus in dual mating strains. Furthermore, Southern blotting demonstrates for the first time the possible presence of a complete MAT1-1 locus in a dual mating strain, instead of the predicted incomplete MAT1-1 locus (dMAT1). Besides the MAT loci, genomic sequences of the BcNEP1 gene in 16 different B. cinerea strains are analyzed, and results demonstrate the existence of three haplotypes and two alleles. Purifying selection is accounted for evolutionary changes within the gene.Finally, four uORF independent knockout mutants were created. This predicted gene is located within the MAT1-1 locus, but its function is yet to be described.Duclos, JoanaKan, Jan vanRepositório da Universidade de LisboaDuarte, José Manuel Martins Abrantes Teixeira2009-02-18T14:57:27Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.5/540enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:31:48Zoai:www.repository.utl.pt:10400.5/540Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:48:48.532408Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
title Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
spellingShingle Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
Duarte, José Manuel Martins Abrantes Teixeira
Botrytis cinerea
genes MAT
proteinas NEP
silenciamento genético
MAT genes
NEP proteins
gene knockout
title_short Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
title_full Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
title_fullStr Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
title_full_unstemmed Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
title_sort Botrytis Cinerea mating types distribution and NEP1 protein sequence analysis
author Duarte, José Manuel Martins Abrantes Teixeira
author_facet Duarte, José Manuel Martins Abrantes Teixeira
author_role author
dc.contributor.none.fl_str_mv Duclos, Joana
Kan, Jan van
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Duarte, José Manuel Martins Abrantes Teixeira
dc.subject.por.fl_str_mv Botrytis cinerea
genes MAT
proteinas NEP
silenciamento genético
MAT genes
NEP proteins
gene knockout
topic Botrytis cinerea
genes MAT
proteinas NEP
silenciamento genético
MAT genes
NEP proteins
gene knockout
description Mestrado em Engenharia Agronómica - Instituto Superior de Agronomia
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2009-02-18T14:57:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/540
url http://hdl.handle.net/10400.5/540
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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