Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.13/3288 |
Resumo: | To unlock the potential of Chlorella stigmatophora (Trebouxiophyceae, Chlorophyta) and Hemiselmis andersenii (Cryptophyceae, Cryptophyta) as natural reactors for biotechnological exploitation, their lipophilic extracts were characterized using Fourier Transform Infrared spectroscopy with Attenuated Total Reflectance (FTIR-ATR) and Gas Chromatography-Mass Spectrometry (GC-MS) before and after alkaline hydrolysis. The GC-MS analysis enabled the identification of 62 metabolites-namely fatty acids (27), aliphatic alcohols (17), monoglycerides (7), sterols (4), and other compounds (7). After alkaline hydrolysis, monounsaturated fatty acids increased by as much as 87%, suggesting that the esterified compounds were mainly neutral lipids. Hemiselmis andersenii yielded the highest Σω3/Σω6 ratio (7.26), indicating that it is a good source of ω3 fatty acids, in comparison to C. stigmatophora (Σω3/Σω6 = 1.24). Both microalgae presented significant amounts of aliphatic alcohols (6.81-10.95 mg · g dw-1 ), which are recognized by their cholesterol-lowering properties. The multivariate analysis allowed visualization of the chemical divergence among H. andersenii lipophilic extracts before and after alkaline hydrolysis, as well as species-specific differences. Chlorella stigmatophora showed to be a valuable source of essential fatty acids for nutraceuticals, whereas H. andersenii, due to its high chemical diversity, seems to be suitable for different fields of application. |
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Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approachChlorella stigmatophoraHemiselmis anderseniiGC-MSLipophilic fractionMicroalgae.Faculdade de Ciências Exatas e da EngenhariaTo unlock the potential of Chlorella stigmatophora (Trebouxiophyceae, Chlorophyta) and Hemiselmis andersenii (Cryptophyceae, Cryptophyta) as natural reactors for biotechnological exploitation, their lipophilic extracts were characterized using Fourier Transform Infrared spectroscopy with Attenuated Total Reflectance (FTIR-ATR) and Gas Chromatography-Mass Spectrometry (GC-MS) before and after alkaline hydrolysis. The GC-MS analysis enabled the identification of 62 metabolites-namely fatty acids (27), aliphatic alcohols (17), monoglycerides (7), sterols (4), and other compounds (7). After alkaline hydrolysis, monounsaturated fatty acids increased by as much as 87%, suggesting that the esterified compounds were mainly neutral lipids. Hemiselmis andersenii yielded the highest Σω3/Σω6 ratio (7.26), indicating that it is a good source of ω3 fatty acids, in comparison to C. stigmatophora (Σω3/Σω6 = 1.24). Both microalgae presented significant amounts of aliphatic alcohols (6.81-10.95 mg · g dw-1 ), which are recognized by their cholesterol-lowering properties. The multivariate analysis allowed visualization of the chemical divergence among H. andersenii lipophilic extracts before and after alkaline hydrolysis, as well as species-specific differences. Chlorella stigmatophora showed to be a valuable source of essential fatty acids for nutraceuticals, whereas H. andersenii, due to its high chemical diversity, seems to be suitable for different fields of application.WileyDigitUMaFernandes, TomásiaCordeiro, Nereida2021-04-15T08:46:58Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.13/3288engFernandes, T., & Cordeiro, N. (2020). Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach. Journal of Phycology, 56(6), 1493-1504.10.1111/jpy.13042info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-19T05:34:22Zoai:digituma.uma.pt:10400.13/3288Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:17.869171Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach |
title |
Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach |
spellingShingle |
Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach Fernandes, Tomásia Chlorella stigmatophora Hemiselmis andersenii GC-MS Lipophilic fraction Microalgae . Faculdade de Ciências Exatas e da Engenharia |
title_short |
Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach |
title_full |
Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach |
title_fullStr |
Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach |
title_full_unstemmed |
Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach |
title_sort |
Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach |
author |
Fernandes, Tomásia |
author_facet |
Fernandes, Tomásia Cordeiro, Nereida |
author_role |
author |
author2 |
Cordeiro, Nereida |
author2_role |
author |
dc.contributor.none.fl_str_mv |
DigitUMa |
dc.contributor.author.fl_str_mv |
Fernandes, Tomásia Cordeiro, Nereida |
dc.subject.por.fl_str_mv |
Chlorella stigmatophora Hemiselmis andersenii GC-MS Lipophilic fraction Microalgae . Faculdade de Ciências Exatas e da Engenharia |
topic |
Chlorella stigmatophora Hemiselmis andersenii GC-MS Lipophilic fraction Microalgae . Faculdade de Ciências Exatas e da Engenharia |
description |
To unlock the potential of Chlorella stigmatophora (Trebouxiophyceae, Chlorophyta) and Hemiselmis andersenii (Cryptophyceae, Cryptophyta) as natural reactors for biotechnological exploitation, their lipophilic extracts were characterized using Fourier Transform Infrared spectroscopy with Attenuated Total Reflectance (FTIR-ATR) and Gas Chromatography-Mass Spectrometry (GC-MS) before and after alkaline hydrolysis. The GC-MS analysis enabled the identification of 62 metabolites-namely fatty acids (27), aliphatic alcohols (17), monoglycerides (7), sterols (4), and other compounds (7). After alkaline hydrolysis, monounsaturated fatty acids increased by as much as 87%, suggesting that the esterified compounds were mainly neutral lipids. Hemiselmis andersenii yielded the highest Σω3/Σω6 ratio (7.26), indicating that it is a good source of ω3 fatty acids, in comparison to C. stigmatophora (Σω3/Σω6 = 1.24). Both microalgae presented significant amounts of aliphatic alcohols (6.81-10.95 mg · g dw-1 ), which are recognized by their cholesterol-lowering properties. The multivariate analysis allowed visualization of the chemical divergence among H. andersenii lipophilic extracts before and after alkaline hydrolysis, as well as species-specific differences. Chlorella stigmatophora showed to be a valuable source of essential fatty acids for nutraceuticals, whereas H. andersenii, due to its high chemical diversity, seems to be suitable for different fields of application. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-04-15T08:46:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.13/3288 |
url |
http://hdl.handle.net/10400.13/3288 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fernandes, T., & Cordeiro, N. (2020). Hemiselmis andersenii and Chlorella stigmatophora as new sources of high‐value compounds: a lipidomic approach. Journal of Phycology, 56(6), 1493-1504. 10.1111/jpy.13042 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799129933530791936 |