Strategies for the hypothermic preservation of cell sheets of human adipose stem cells
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/74743 |
Resumo: | Cell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenges related with the preservation of CS structure and function from the fabrication site to the bedside. Pausing cells at hypothermic temperatures has been suggested as a valuable method for short-term cell preservation. In this study, we tested the efficiency of two preservation strategies, one using culture medium supplementation with Rokepie and the other using the preservation solution Hypothermosol, in preserving human adipose stromal/stem cells (hASC) CS-like confluent cultures at 4°C, during 3 and 7 days. Both preservation strategies demonstrated excellent ability to preserve cell function during the first 3 days in hypothermia, as demonstrated by metabolic activity results and assessment of extracellular matrix integrity and differentiation potential. At the end of the 7th day of hypothermic incubation, the decrease in cell metabolic activity was more evident for all conditions. Nonetheless, hASC incubated with Rokepie and Hypothermosol retained a higher metabolic activity and extracellular matrix integrity in comparison with unsupplemented cells. Differentiation results for the later time point showed that supplementation with both Rokepie and Hypothermosol rescued adipogenic differentiation potential but only Rokepie was able to preserve hASC osteogenic potential. |
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Strategies for the hypothermic preservation of cell sheets of human adipose stem cellsCell sheetsHuman adipose stem cellsHypothermic preservationScience & TechnologyCell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenges related with the preservation of CS structure and function from the fabrication site to the bedside. Pausing cells at hypothermic temperatures has been suggested as a valuable method for short-term cell preservation. In this study, we tested the efficiency of two preservation strategies, one using culture medium supplementation with Rokepie and the other using the preservation solution Hypothermosol, in preserving human adipose stromal/stem cells (hASC) CS-like confluent cultures at 4°C, during 3 and 7 days. Both preservation strategies demonstrated excellent ability to preserve cell function during the first 3 days in hypothermia, as demonstrated by metabolic activity results and assessment of extracellular matrix integrity and differentiation potential. At the end of the 7th day of hypothermic incubation, the decrease in cell metabolic activity was more evident for all conditions. Nonetheless, hASC incubated with Rokepie and Hypothermosol retained a higher metabolic activity and extracellular matrix integrity in comparison with unsupplemented cells. Differentiation results for the later time point showed that supplementation with both Rokepie and Hypothermosol rescued adipogenic differentiation potential but only Rokepie was able to preserve hASC osteogenic potential.This work was supported by: SF-R by PhD grant PD/BD/135252/2017; AFC by contract financed by SFRH/BPD/109595/2015; MTC by NORTE-01-0145-FEDER-000021 and RPP by IF/ 00347/2015, all supported by Fundac¸ão para a Ciencia e Tecnologia (FCT). This work was supported by LA ICVS/3B’s project UID/Multi/ 50026/2013 (POCI-01-0145-FEDER-007038), financed by FCT and Fundo Europeu de Desenvolvimento Regional (FEDER), as well as Gene2Skin Project (H2020-TWINN-2015-692221). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Public Library of Science (PLOS)Universidade do MinhoFreitas-Ribeiro, SaraCarvalho, Andreia FilipaCosta, MarinaCerqueira, Mariana TeixeiraMarques, A. P.Reis, R. L.Pirraco, Rogério P.2019-102019-10-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/74743engFreitas-Ribeiro S., Carvalho A. F., Costa M., Cerqueira M. T., Marques A. P., Reis R. L., Pirraco R. P. Strategies for the hypothermic preservation of cell sheets of human adipose stem cells, Plos One, doi:10.1371/JOURNAL.PONE.0222597, 20191932-620310.1371/journal.pone.022259731613935https://doi.org/10.1371/journal.pone.0222597info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:42:05Zoai:repositorium.sdum.uminho.pt:1822/74743Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:42:05Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Strategies for the hypothermic preservation of cell sheets of human adipose stem cells |
title |
Strategies for the hypothermic preservation of cell sheets of human adipose stem cells |
spellingShingle |
Strategies for the hypothermic preservation of cell sheets of human adipose stem cells Freitas-Ribeiro, Sara Cell sheets Human adipose stem cells Hypothermic preservation Science & Technology |
title_short |
Strategies for the hypothermic preservation of cell sheets of human adipose stem cells |
title_full |
Strategies for the hypothermic preservation of cell sheets of human adipose stem cells |
title_fullStr |
Strategies for the hypothermic preservation of cell sheets of human adipose stem cells |
title_full_unstemmed |
Strategies for the hypothermic preservation of cell sheets of human adipose stem cells |
title_sort |
Strategies for the hypothermic preservation of cell sheets of human adipose stem cells |
author |
Freitas-Ribeiro, Sara |
author_facet |
Freitas-Ribeiro, Sara Carvalho, Andreia Filipa Costa, Marina Cerqueira, Mariana Teixeira Marques, A. P. Reis, R. L. Pirraco, Rogério P. |
author_role |
author |
author2 |
Carvalho, Andreia Filipa Costa, Marina Cerqueira, Mariana Teixeira Marques, A. P. Reis, R. L. Pirraco, Rogério P. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Freitas-Ribeiro, Sara Carvalho, Andreia Filipa Costa, Marina Cerqueira, Mariana Teixeira Marques, A. P. Reis, R. L. Pirraco, Rogério P. |
dc.subject.por.fl_str_mv |
Cell sheets Human adipose stem cells Hypothermic preservation Science & Technology |
topic |
Cell sheets Human adipose stem cells Hypothermic preservation Science & Technology |
description |
Cell Sheet (CS) Engineering is a regenerative medicine strategy proposed for the treatment of injured or diseased organs and tissues. In fact, several clinical trials are underway using CS-based methodologies. However, the clinical application of such cell-based methodologies poses several challenges related with the preservation of CS structure and function from the fabrication site to the bedside. Pausing cells at hypothermic temperatures has been suggested as a valuable method for short-term cell preservation. In this study, we tested the efficiency of two preservation strategies, one using culture medium supplementation with Rokepie and the other using the preservation solution Hypothermosol, in preserving human adipose stromal/stem cells (hASC) CS-like confluent cultures at 4°C, during 3 and 7 days. Both preservation strategies demonstrated excellent ability to preserve cell function during the first 3 days in hypothermia, as demonstrated by metabolic activity results and assessment of extracellular matrix integrity and differentiation potential. At the end of the 7th day of hypothermic incubation, the decrease in cell metabolic activity was more evident for all conditions. Nonetheless, hASC incubated with Rokepie and Hypothermosol retained a higher metabolic activity and extracellular matrix integrity in comparison with unsupplemented cells. Differentiation results for the later time point showed that supplementation with both Rokepie and Hypothermosol rescued adipogenic differentiation potential but only Rokepie was able to preserve hASC osteogenic potential. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10 2019-10-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/74743 |
url |
http://hdl.handle.net/1822/74743 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Freitas-Ribeiro S., Carvalho A. F., Costa M., Cerqueira M. T., Marques A. P., Reis R. L., Pirraco R. P. Strategies for the hypothermic preservation of cell sheets of human adipose stem cells, Plos One, doi:10.1371/JOURNAL.PONE.0222597, 2019 1932-6203 10.1371/journal.pone.0222597 31613935 https://doi.org/10.1371/journal.pone.0222597 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library of Science (PLOS) |
publisher.none.fl_str_mv |
Public Library of Science (PLOS) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817544391428931584 |