A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/58272 |
Resumo: | Accepted Manuscript |
| id |
RCAP_02dafdbe57916e2b21d675b0df286edd |
|---|---|
| oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/58272 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9Ciências Médicas::Medicina BásicaScience & TechnologyAccepted ManuscriptThe challenge of the early diagnosis of pancreatic cancer in routine clinical practice requires low-cost means of detection, and this may be achieved with immunosensors based on electrical or electrochemical principles. In this paper, we report a potentially low-cost immunosensor built with interdigitated gold electrodes coated with a self-assembled monolayer and a layer of anti-CA19-9 antibodies, which is capable of detecting the pancreatic cancer biomarker CA19-9 using electrical impedance spectroscopy. Due to specific, irreversible adsorption of CA19-9 onto its corresponding antibody, according to data from polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS), the immunosensor is highly sensitive and selective. It could detect CA19-9 in commercial samples with a limit of detection of 0.68 U mL−1, in addition to distinguishing between blood serum samples from patients with different concentrations of CA19-9. Furthermore, by treating the capacitance data with information visualization methods, we were able to verify the selectivity and robustness of the immunosensor with regard to false positives, as the samples containing higher CA19-9 concentrations, including those from tumor cells, could be distinguished from those with possible interferents.CAPES, FAPESP (Grant 2013/14262-7 and 2012/15543-7), CNPq (150985/2017-7), nBioNet network and Barretos Cancer Hospital for financial supportinfo:eu-repo/semantics/publishedVersionRoyal Society of ChemistryUniversidade do MinhoSoares, Andrey CoatriniSoares, Juliana CoatriniShimizu, Flavio MakotoRodrigues, Valquiria da CruzAwan, Iram TajMelendez, Matias EliseoPiazzetta, Maria Helena OliveiraGobbi, Angelo LuizReis, R. M.Fregnani, José Humberto T. G.Carvalho, André LopesOliveira, Osvaldo N.20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58272engSoares, A. C., Soares, J. C., Shimizu, F. M., et. al. (2018). A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9. Analyst0003-26541364-552810.1039/C8AN00430G29756151https://pubs.rsc.org/en/content/articlehtml/2018/an/c8an00430ginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:13:35Zoai:repositorium.sdum.uminho.pt:1822/58272Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:05:44.337129Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9 |
| title |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9 |
| spellingShingle |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9 Soares, Andrey Coatrini Ciências Médicas::Medicina Básica Science & Technology |
| title_short |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9 |
| title_full |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9 |
| title_fullStr |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9 |
| title_full_unstemmed |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9 |
| title_sort |
A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9 |
| author |
Soares, Andrey Coatrini |
| author_facet |
Soares, Andrey Coatrini Soares, Juliana Coatrini Shimizu, Flavio Makoto Rodrigues, Valquiria da Cruz Awan, Iram Taj Melendez, Matias Eliseo Piazzetta, Maria Helena Oliveira Gobbi, Angelo Luiz Reis, R. M. Fregnani, José Humberto T. G. Carvalho, André Lopes Oliveira, Osvaldo N. |
| author_role |
author |
| author2 |
Soares, Juliana Coatrini Shimizu, Flavio Makoto Rodrigues, Valquiria da Cruz Awan, Iram Taj Melendez, Matias Eliseo Piazzetta, Maria Helena Oliveira Gobbi, Angelo Luiz Reis, R. M. Fregnani, José Humberto T. G. Carvalho, André Lopes Oliveira, Osvaldo N. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Soares, Andrey Coatrini Soares, Juliana Coatrini Shimizu, Flavio Makoto Rodrigues, Valquiria da Cruz Awan, Iram Taj Melendez, Matias Eliseo Piazzetta, Maria Helena Oliveira Gobbi, Angelo Luiz Reis, R. M. Fregnani, José Humberto T. G. Carvalho, André Lopes Oliveira, Osvaldo N. |
| dc.subject.por.fl_str_mv |
Ciências Médicas::Medicina Básica Science & Technology |
| topic |
Ciências Médicas::Medicina Básica Science & Technology |
| description |
Accepted Manuscript |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/58272 |
| url |
http://hdl.handle.net/1822/58272 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Soares, A. C., Soares, J. C., Shimizu, F. M., et. al. (2018). A simple architecture with self-assembled monolayers to build immunosensors for detecting the pancreatic cancer biomarker CA19-9. Analyst 0003-2654 1364-5528 10.1039/C8AN00430G 29756151 https://pubs.rsc.org/en/content/articlehtml/2018/an/c8an00430g |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
| publisher.none.fl_str_mv |
Royal Society of Chemistry |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799132469835857920 |