A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10451/48920 |
Resumo: | The increasing uncontrolled development of human activities and consequent increase in the production and release into the marine realm of potentially harmful substances highlights the need to develop efficient and high-throughput screening (HTS) tools. Bio-optical tools, such as laser-induced fluorescence (LIF) and pulse amplitude modulated (PAM) fluorometry, emerge as efficient non-invasive techniques for toxicophenomic evaluation in ecotoxicological trials. Both techniques generate large datasets that can be applied in multivariate analysis to evaluate canonical classification efficiency of the exposure types and levels to which photosynthetic organisms, such as diatoms, are subjected to. In the present work, marine diatom cultures were exposed to two trace elements known to have physiological roles and different toxicity ranges (Zn and Cu), and to two other trace elements without known metabolic functions and very different toxicological profiles (Cr and Hg). All the tested approaches were able to disentangle the control groups from the test groups. Moreover, the application of LIF raw-data showed that this technique had the higher classification efficiency, providing very good separation of the different doses applied of each trace element tested. Additionally, PAM chlorophyll fast induction kinetics raw data also produced good classification efficiencies and provided data that can be useful for interpreting the physiological shifts induced by trace element exposure. In sum, LIF and PAM techniques appear as completely non-invasive HTS techniques that, when applied together, produce a correct toxicophenomic classification and disentangle the physiological effects behind the observed phenomic changes. Therefore, they are worth to be included in future ecotoxicological assessment test protocols. |
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A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatomTrace elementsChlorophyll fluorescenceEcotoxicologyHigh-throughput screeningThe increasing uncontrolled development of human activities and consequent increase in the production and release into the marine realm of potentially harmful substances highlights the need to develop efficient and high-throughput screening (HTS) tools. Bio-optical tools, such as laser-induced fluorescence (LIF) and pulse amplitude modulated (PAM) fluorometry, emerge as efficient non-invasive techniques for toxicophenomic evaluation in ecotoxicological trials. Both techniques generate large datasets that can be applied in multivariate analysis to evaluate canonical classification efficiency of the exposure types and levels to which photosynthetic organisms, such as diatoms, are subjected to. In the present work, marine diatom cultures were exposed to two trace elements known to have physiological roles and different toxicity ranges (Zn and Cu), and to two other trace elements without known metabolic functions and very different toxicological profiles (Cr and Hg). All the tested approaches were able to disentangle the control groups from the test groups. Moreover, the application of LIF raw-data showed that this technique had the higher classification efficiency, providing very good separation of the different doses applied of each trace element tested. Additionally, PAM chlorophyll fast induction kinetics raw data also produced good classification efficiencies and provided data that can be useful for interpreting the physiological shifts induced by trace element exposure. In sum, LIF and PAM techniques appear as completely non-invasive HTS techniques that, when applied together, produce a correct toxicophenomic classification and disentangle the physiological effects behind the observed phenomic changes. Therefore, they are worth to be included in future ecotoxicological assessment test protocols.ElsevierRepositório da Universidade de LisboaDuarte, BernardoGameiro, CarlaUtkin, Andrei BorissovitchMatos, Ana RitaCaçador, IsabelFonseca, VanessaCabrita, Maria Teresa2021-07-14T10:21:47Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/48920engDuarte, B., Gameiro, C., Utkin, A. B., ... Fonseca, V. & Cabrita, M. T. (2021). A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom. Estuarine, Coastal and Shelf Science, 250, 107170. https://doi.org/10.1016/j.ecss.2021.1071700272-771410.1016/j.ecss.2021.107170metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:52:31Zoai:repositorio.ul.pt:10451/48920Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:00:41.961875Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom |
title |
A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom |
spellingShingle |
A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom Duarte, Bernardo Trace elements Chlorophyll fluorescence Ecotoxicology High-throughput screening |
title_short |
A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom |
title_full |
A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom |
title_fullStr |
A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom |
title_full_unstemmed |
A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom |
title_sort |
A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom |
author |
Duarte, Bernardo |
author_facet |
Duarte, Bernardo Gameiro, Carla Utkin, Andrei Borissovitch Matos, Ana Rita Caçador, Isabel Fonseca, Vanessa Cabrita, Maria Teresa |
author_role |
author |
author2 |
Gameiro, Carla Utkin, Andrei Borissovitch Matos, Ana Rita Caçador, Isabel Fonseca, Vanessa Cabrita, Maria Teresa |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Duarte, Bernardo Gameiro, Carla Utkin, Andrei Borissovitch Matos, Ana Rita Caçador, Isabel Fonseca, Vanessa Cabrita, Maria Teresa |
dc.subject.por.fl_str_mv |
Trace elements Chlorophyll fluorescence Ecotoxicology High-throughput screening |
topic |
Trace elements Chlorophyll fluorescence Ecotoxicology High-throughput screening |
description |
The increasing uncontrolled development of human activities and consequent increase in the production and release into the marine realm of potentially harmful substances highlights the need to develop efficient and high-throughput screening (HTS) tools. Bio-optical tools, such as laser-induced fluorescence (LIF) and pulse amplitude modulated (PAM) fluorometry, emerge as efficient non-invasive techniques for toxicophenomic evaluation in ecotoxicological trials. Both techniques generate large datasets that can be applied in multivariate analysis to evaluate canonical classification efficiency of the exposure types and levels to which photosynthetic organisms, such as diatoms, are subjected to. In the present work, marine diatom cultures were exposed to two trace elements known to have physiological roles and different toxicity ranges (Zn and Cu), and to two other trace elements without known metabolic functions and very different toxicological profiles (Cr and Hg). All the tested approaches were able to disentangle the control groups from the test groups. Moreover, the application of LIF raw-data showed that this technique had the higher classification efficiency, providing very good separation of the different doses applied of each trace element tested. Additionally, PAM chlorophyll fast induction kinetics raw data also produced good classification efficiencies and provided data that can be useful for interpreting the physiological shifts induced by trace element exposure. In sum, LIF and PAM techniques appear as completely non-invasive HTS techniques that, when applied together, produce a correct toxicophenomic classification and disentangle the physiological effects behind the observed phenomic changes. Therefore, they are worth to be included in future ecotoxicological assessment test protocols. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-07-14T10:21:47Z 2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10451/48920 |
url |
http://hdl.handle.net/10451/48920 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Duarte, B., Gameiro, C., Utkin, A. B., ... Fonseca, V. & Cabrita, M. T. (2021). A multivariate approach to chlorophyll a fluorescence data for trace element ecotoxicological trials using a model marine diatom. Estuarine, Coastal and Shelf Science, 250, 107170. https://doi.org/10.1016/j.ecss.2021.107170 0272-7714 10.1016/j.ecss.2021.107170 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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