Using zeta-potential measurements to quantify peptide partition to lipid membranes

Detalhes bibliográficos
Autor(a) principal: Freire, João M.
Data de Publicação: 2011
Outros Autores: Domingues, Marco M., Matos, Joana, Melo, Manuel N., Veiga, Ana Salomé, Santos, Nuno C., Castanho, Miguel A. R. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/6743
Resumo: © The Author(s) 2011. This article is published with open access at Springerlink.com.
id RCAP_033a492aeed49cf2ca51e4a0953f7be3
oai_identifier_str oai:repositorio.ul.pt:10451/6743
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Using zeta-potential measurements to quantify peptide partition to lipid membranesPartition constantZeta-potentialFluorescenceMembraneAntimicrobial peptides© The Author(s) 2011. This article is published with open access at Springerlink.com.Open Access: This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.Many cellular phenomena occur on the biomembranes. There are plenty of molecules (natural or xenobiotics) that interact directly or partially with the cell membrane. Biomolecules, such as several peptides (e.g., antimicrobial peptides) and proteins, exert their effects at the cell membrane level. This feature makes necessary investigating their interactions with lipids to clarify their mechanisms of action and side effects necessary. The determination of molecular lipid/water partition constants (Kp) is frequently used to quantify the extension of the interaction. The determination of this parameter has been achieved by using different methodologies, such as UV-Vis absorption spectrophotometry, fluorescence spectroscopy and ζ-potential measurements. In this work, we derived and tested a mathematical model to determine the Kp from ζ-potential data. The values obtained with this method were compared with those obtained by fluorescence spectroscopy, which is a regular technique used to quantify the interaction of intrinsically fluorescent peptides with selected biomembrane model systems. Two antimicrobial peptides (BP100 and pepR) were evaluated by this new method. The results obtained by this new methodology show that ζ-potential is a powerful technique to quantify peptide/lipid interactions of a wide variety of charged molecules, overcoming some of the limitations inherent to other techniques, such as the need for fluorescent labeling.This work was partially supported by project PTDC/QUI/ 69937/2006 from Fundação para a Ciência e Tecnologia-Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal), and by Fundação Calouste Gulbenkian (Portugal). JMF and MMD also thank FCT-MCTES for grants IMM/BT/37-2010 and SFRH/BD/41750/2007, respectively.SpringerRepositório da Universidade de LisboaFreire, João M.Domingues, Marco M.Matos, JoanaMelo, Manuel N.Veiga, Ana SaloméSantos, Nuno C.Castanho, Miguel A. R. B.2012-07-23T12:00:01Z20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/6743engEur Biophys J (2011) 40:481–4870175-7571http://dx.doi.org/10.1007/s00249-010-0661-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:49:12Zoai:repositorio.ul.pt:10451/6743Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:31:40.840301Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Using zeta-potential measurements to quantify peptide partition to lipid membranes
title Using zeta-potential measurements to quantify peptide partition to lipid membranes
spellingShingle Using zeta-potential measurements to quantify peptide partition to lipid membranes
Freire, João M.
Partition constant
Zeta-potential
Fluorescence
Membrane
Antimicrobial peptides
title_short Using zeta-potential measurements to quantify peptide partition to lipid membranes
title_full Using zeta-potential measurements to quantify peptide partition to lipid membranes
title_fullStr Using zeta-potential measurements to quantify peptide partition to lipid membranes
title_full_unstemmed Using zeta-potential measurements to quantify peptide partition to lipid membranes
title_sort Using zeta-potential measurements to quantify peptide partition to lipid membranes
author Freire, João M.
author_facet Freire, João M.
Domingues, Marco M.
Matos, Joana
Melo, Manuel N.
Veiga, Ana Salomé
Santos, Nuno C.
Castanho, Miguel A. R. B.
author_role author
author2 Domingues, Marco M.
Matos, Joana
Melo, Manuel N.
Veiga, Ana Salomé
Santos, Nuno C.
Castanho, Miguel A. R. B.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Freire, João M.
Domingues, Marco M.
Matos, Joana
Melo, Manuel N.
Veiga, Ana Salomé
Santos, Nuno C.
Castanho, Miguel A. R. B.
dc.subject.por.fl_str_mv Partition constant
Zeta-potential
Fluorescence
Membrane
Antimicrobial peptides
topic Partition constant
Zeta-potential
Fluorescence
Membrane
Antimicrobial peptides
description © The Author(s) 2011. This article is published with open access at Springerlink.com.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
2012-07-23T12:00:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/6743
url http://hdl.handle.net/10451/6743
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Eur Biophys J (2011) 40:481–487
0175-7571
http://dx.doi.org/10.1007/s00249-010-0661-4
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799134207448973312

Registros relacionados