Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB

Detalhes bibliográficos
Autor(a) principal: Escrevente, Cristina
Data de Publicação: 2021
Outros Autores: Bento-Lopes, Liliana, Ramalho, José S, Barral, Duarte C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/120539
Resumo: Funding: This study was supported by Fundaçã o para a Ciência e Tecnologia (FCT): C.E. was supported by a post-doctoral fellowship (SFRH/BPD/78491/2011), L.B.-L. by a PhD fellowship (SFRH/BD/131938/2017) and D.C.B. by the FCT Investigator Program (IF/00501/2014/CP1252/CT0001). This work was developed with the support from the research infrastructure PPBI-POCI-01-0145-FEDER-022122, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund). This article was supported by the LYSOCIL project. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 811087. Deposited in PMC for immediate release.
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spelling Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRABExocytosisGRABRAB3IL1LysosomeRab11Rab3aSec15Funding: This study was supported by Fundaçã o para a Ciência e Tecnologia (FCT): C.E. was supported by a post-doctoral fellowship (SFRH/BPD/78491/2011), L.B.-L. by a PhD fellowship (SFRH/BD/131938/2017) and D.C.B. by the FCT Investigator Program (IF/00501/2014/CP1252/CT0001). This work was developed with the support from the research infrastructure PPBI-POCI-01-0145-FEDER-022122, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund). This article was supported by the LYSOCIL project. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 811087. Deposited in PMC for immediate release.Lysosomes are dynamic organelles, capable of undergoing exocytosis. This process is crucial for several cellular functions, namely plasma membrane repair. Nevertheless, the molecular machinery involved in this process is poorly understood. Here, we identify Rab11a and Rab11b as regulators of calcium-induced lysosome exocytosis. Interestingly, Rab11-positive vesicles transiently interact with lysosomes at the cell periphery, indicating that this interaction is required for the last steps of lysosome exocytosis. Additionally, we found that the silencing of the exocyst subunit Sec15, a Rab11 effector, impairs lysosome exocytosis, suggesting that Sec15 acts together with Rab11 in the regulation of lysosome exocytosis. Furthermore, we show that Rab11 binds the guanine nucleotide exchange factor for Rab3a (GRAB) and also Rab3a, which we described previously as a regulator of the positioning and exocytosis of lysosomes. Thus, our study identifies new players required for lysosome exocytosis and suggest the existence of a Rab11-Rab3a cascade involved in this process.iNOVA4Health - pólo NMSNOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNEscrevente, CristinaBento-Lopes, LilianaRamalho, José SBarral, Duarte C2021-07-05T22:18:09Z2021-06-082021-06-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/120539eng0021-9533PURE: 31942675https://doi.org/10.1242/jcs.246694info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:03:01Zoai:run.unl.pt:10362/120539Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:22.566548Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB
title Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB
spellingShingle Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB
Escrevente, Cristina
Exocytosis
GRAB
RAB3IL1
Lysosome
Rab11
Rab3a
Sec15
title_short Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB
title_full Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB
title_fullStr Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB
title_full_unstemmed Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB
title_sort Rab11 is required for lysosome exocytosis through the interaction with Rab3a, Sec15 and GRAB
author Escrevente, Cristina
author_facet Escrevente, Cristina
Bento-Lopes, Liliana
Ramalho, José S
Barral, Duarte C
author_role author
author2 Bento-Lopes, Liliana
Ramalho, José S
Barral, Duarte C
author2_role author
author
author
dc.contributor.none.fl_str_mv iNOVA4Health - pólo NMS
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
RUN
dc.contributor.author.fl_str_mv Escrevente, Cristina
Bento-Lopes, Liliana
Ramalho, José S
Barral, Duarte C
dc.subject.por.fl_str_mv Exocytosis
GRAB
RAB3IL1
Lysosome
Rab11
Rab3a
Sec15
topic Exocytosis
GRAB
RAB3IL1
Lysosome
Rab11
Rab3a
Sec15
description Funding: This study was supported by Fundaçã o para a Ciência e Tecnologia (FCT): C.E. was supported by a post-doctoral fellowship (SFRH/BPD/78491/2011), L.B.-L. by a PhD fellowship (SFRH/BD/131938/2017) and D.C.B. by the FCT Investigator Program (IF/00501/2014/CP1252/CT0001). This work was developed with the support from the research infrastructure PPBI-POCI-01-0145-FEDER-022122, co-financed by FCT (Portugal) and Lisboa2020, under the PORTUGAL2020 agreement (European Regional Development Fund). This article was supported by the LYSOCIL project. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 811087. Deposited in PMC for immediate release.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-05T22:18:09Z
2021-06-08
2021-06-08T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/120539
url http://hdl.handle.net/10362/120539
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0021-9533
PURE: 31942675
https://doi.org/10.1242/jcs.246694
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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