Cell Model of Depression: Reduction of Cell Stress with Mirtazapine

Detalhes bibliográficos
Autor(a) principal: Correia, Ana Salomé
Data de Publicação: 2022
Outros Autores: Fraga, Sónia, Teixeira, João Paulo, Vale, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8301
Resumo: This article belongs to the Special Issue Blood-Brain Barrier in CNS Injury and Repair 2022
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spelling Cell Model of Depression: Reduction of Cell Stress with MirtazapineDepressionSH-SY5YComet AssayMirtazapineHydrogen PeroxideStressTecnologias de Análise de DNAThis article belongs to the Special Issue Blood-Brain Barrier in CNS Injury and Repair 2022Depression is a very prevalent and complex disease. This condition is associated with a high rate of relapse, making its treatment a challenge. Thus, an intensive investigation of this disease and its treatment is necessary. In this work, through cell viability assays (MTT and neutral red assays) and alkaline comet assays, we aimed to test the induction of stress in human SH-SY5Y cells through the application of hydrocortisone and hydrogen peroxide and to test the reversal or attenuation of this stress through the application of mirtazapine to the cells. Our results demonstrated that hydrogen peroxide, and not hydrocortisone, can induce cellular stress, as evidenced by DNA damage and a global cellular viability reduction, which were alleviated by the antidepressant mirtazapine. The establishment of a cellular model of depression through stress induction is important to study new possibilities of treatment of this disease using cell cultures.This research was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation (POCI), Portugal 2020, and by Portuguese funds through Fundação para a Ciência e a Tecnologia (FCT) in the framework of the project IF/00092/2014/CP1255/CT0004 and CHAIR in Onco-Innovation.MDPIRepositório Científico do Instituto Nacional de SaúdeCorreia, Ana SaloméFraga, SóniaTeixeira, João PauloVale, Nuno2022-11-03T12:39:55Z2022-04-292022-04-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8301engInt J Mol Sci . 2022 Apr 29;23(9):4942. doi: 10.3390/ijms230949421661-659610.3390/ijms23094942info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:29Zoai:repositorio.insa.pt:10400.18/8301Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:57.187916Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
title Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
spellingShingle Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
Correia, Ana Salomé
Depression
SH-SY5Y
Comet Assay
Mirtazapine
Hydrogen Peroxide
Stress
Tecnologias de Análise de DNA
title_short Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
title_full Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
title_fullStr Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
title_full_unstemmed Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
title_sort Cell Model of Depression: Reduction of Cell Stress with Mirtazapine
author Correia, Ana Salomé
author_facet Correia, Ana Salomé
Fraga, Sónia
Teixeira, João Paulo
Vale, Nuno
author_role author
author2 Fraga, Sónia
Teixeira, João Paulo
Vale, Nuno
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Correia, Ana Salomé
Fraga, Sónia
Teixeira, João Paulo
Vale, Nuno
dc.subject.por.fl_str_mv Depression
SH-SY5Y
Comet Assay
Mirtazapine
Hydrogen Peroxide
Stress
Tecnologias de Análise de DNA
topic Depression
SH-SY5Y
Comet Assay
Mirtazapine
Hydrogen Peroxide
Stress
Tecnologias de Análise de DNA
description This article belongs to the Special Issue Blood-Brain Barrier in CNS Injury and Repair 2022
publishDate 2022
dc.date.none.fl_str_mv 2022-11-03T12:39:55Z
2022-04-29
2022-04-29T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8301
url http://hdl.handle.net/10400.18/8301
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Int J Mol Sci . 2022 Apr 29;23(9):4942. doi: 10.3390/ijms23094942
1661-6596
10.3390/ijms23094942
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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