Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains

Detalhes bibliográficos
Autor(a) principal: Mendoza, Hector
Data de Publicação: 2008
Outros Autores: De la Jara, Adelina, Freijanes, Karen, Carmona, Laura, Ramos, Ana, Duarte, Vanessa, Varela, J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/4836
Resumo: In the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye Nile red allowed the development of a cytometric method faster than the traditional techniques to select carotenoid hyperproducing strains of microalgae.
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spelling Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strainsFlow cytometryCarotenoidsDunaliella salinaMicroalgaeIn the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye Nile red allowed the development of a cytometric method faster than the traditional techniques to select carotenoid hyperproducing strains of microalgae.FCTOVERCAROTEN POCTI/MAR/15237/99; INTERREG 159-SAL - Atlantic Salt Ponds.SapientiaMendoza, HectorDe la Jara, AdelinaFreijanes, KarenCarmona, LauraRamos, AnaDuarte, VanessaVarela, J.2014-07-23T16:51:09Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/4836engMendoza, H., K. Freijanes, L. Carmona, A. Ramos, V. Duarte, J. Varela & A. de la Jara. (2008). Characterization of Dunaliella salina strains by flow cytometry: a new technique to select β-carotene hyperproducing strains. Electronic Journal of Biotechnology, 11 (4): doi:10.2225/vol11-issue4-fulltext-2.0717-3458AUT: JVA01305info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:15:58Zoai:sapientia.ualg.pt:10400.1/4836Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:58:01.910254Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
title Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
spellingShingle Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
Mendoza, Hector
Flow cytometry
Carotenoids
Dunaliella salina
Microalgae
title_short Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
title_full Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
title_fullStr Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
title_full_unstemmed Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
title_sort Characterization of Dunaliella salina strains by flow cytometry: a new approach to select carotenoid hyperproducing strains
author Mendoza, Hector
author_facet Mendoza, Hector
De la Jara, Adelina
Freijanes, Karen
Carmona, Laura
Ramos, Ana
Duarte, Vanessa
Varela, J.
author_role author
author2 De la Jara, Adelina
Freijanes, Karen
Carmona, Laura
Ramos, Ana
Duarte, Vanessa
Varela, J.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Mendoza, Hector
De la Jara, Adelina
Freijanes, Karen
Carmona, Laura
Ramos, Ana
Duarte, Vanessa
Varela, J.
dc.subject.por.fl_str_mv Flow cytometry
Carotenoids
Dunaliella salina
Microalgae
topic Flow cytometry
Carotenoids
Dunaliella salina
Microalgae
description In the present work the characterization of different strains of Dunaliella salina from established cell culture collections and various isolates from solar saltworks located in the Canary Island, as well as one mutant, was carried out in order to assess the existence of intraspecific differences and to determine the potential productivity of each one. Morphological characteristics such as cellular size and cellular complexity and parameters linked to cellular physiology, such as pigment content or cellular growth rate, were determined by means of traditional techniques as well as flow cytometry. Results showed a high morphological and physiological intraspecific variability among the studied strains. Results suggest that the application of the lipidic dye Nile red allowed the development of a cytometric method faster than the traditional techniques to select carotenoid hyperproducing strains of microalgae.
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2014-07-23T16:51:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/4836
url http://hdl.handle.net/10400.1/4836
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mendoza, H., K. Freijanes, L. Carmona, A. Ramos, V. Duarte, J. Varela & A. de la Jara. (2008). Characterization of Dunaliella salina strains by flow cytometry: a new technique to select β-carotene hyperproducing strains. Electronic Journal of Biotechnology, 11 (4): doi:10.2225/vol11-issue4-fulltext-2.
0717-3458
AUT: JVA01305
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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