Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens

Detalhes bibliográficos
Autor(a) principal: Ciurko, Dominika
Data de Publicação: 2023
Outros Autores: Laba, Wojciech, Kancelista, Anna, John, Lukasz, Gudiña, Eduardo José, Lazar, Zbigniew, Janek, Tomasz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/84645
Resumo: Supplementary data associated with this article can be found in the online version at doi:10.1016/j.bej.2023.108981.
id RCAP_03cc01d2d7eef5d13a7fc7e83d457146
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/84645
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescensblack cumin cakePseudomonas fluorescenslipopeptidesoptimization studiesantifungal activitySupplementary data associated with this article can be found in the online version at doi:10.1016/j.bej.2023.108981.Most biosurfactants are obtained using costly culture media, which limits their wider industrial use. In the present study, a low-cost culture medium, containing the agro-industrial residue black cumin cake, was developed for amphisin production by Pseudomonas fluorescens DSS73. By using black cumin cake as the substrate, not only was the production cost reduced but also a higher production yield was achieved. A Box-Behnken experimental design was applied to maximize lipopeptide biosurfactant production. The optimal conditions for amphisin production, such as black cumin cake (6.6%) and NaCl (8.0 mM) concentration, and cultivation time (6.5 days), were determined. Yield of amphisin production, performed in optimal conditions, reached 16.51 ± 0.49 g/L. Such high production has not been evidenced previously for Pseudomonas lipopeptide biosurfactants. Moreover, active utilization of the substrate, observed with the aid of scanning electron microscopy (SEM), documented by numerous holes and pitting on the black cumin cake surface, was confirmed. Finally, antifungal activity of amphisin against Aspergillus carbonarius was demonstrated. Hence, amphisin production by P. fluorescens was achieved with statistical optimization using an inexpensive agro-industrial by-product for the first time.This work was supported by the National Science Centre, Poland, project 2020/37/B/NZ9/01519. EJG would like to acknowledge the support of the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit, and by LABBELS – Associate Laboratory in Biotechnology, Bioengineering and Microelectromechanical Systems, LA/P/0029/2020.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoCiurko, DominikaLaba, WojciechKancelista, AnnaJohn, LukaszGudiña, Eduardo JoséLazar, ZbigniewJanek, Tomasz2023-082023-08-01T00:00:00Z10000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/84645engCiurko, D., Łaba, W., Kancelista, A., John, Ł., Gudiña, E. J., Lazar, Z., & Janek, T. (2023, August). Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens. Biochemical Engineering Journal. Elsevier BV. http://doi.org/10.1016/j.bej.2023.1089811369-703X10.1016/j.bej.2023.108981108981http://www.elsevier.com/locate/issn/1369703Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:27:18Zoai:repositorium.sdum.uminho.pt:1822/84645Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:21:50.901888Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens
title Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens
spellingShingle Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens
Ciurko, Dominika
black cumin cake
Pseudomonas fluorescens
lipopeptides
optimization studies
antifungal activity
title_short Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens
title_full Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens
title_fullStr Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens
title_full_unstemmed Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens
title_sort Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens
author Ciurko, Dominika
author_facet Ciurko, Dominika
Laba, Wojciech
Kancelista, Anna
John, Lukasz
Gudiña, Eduardo José
Lazar, Zbigniew
Janek, Tomasz
author_role author
author2 Laba, Wojciech
Kancelista, Anna
John, Lukasz
Gudiña, Eduardo José
Lazar, Zbigniew
Janek, Tomasz
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Ciurko, Dominika
Laba, Wojciech
Kancelista, Anna
John, Lukasz
Gudiña, Eduardo José
Lazar, Zbigniew
Janek, Tomasz
dc.subject.por.fl_str_mv black cumin cake
Pseudomonas fluorescens
lipopeptides
optimization studies
antifungal activity
topic black cumin cake
Pseudomonas fluorescens
lipopeptides
optimization studies
antifungal activity
description Supplementary data associated with this article can be found in the online version at doi:10.1016/j.bej.2023.108981.
publishDate 2023
dc.date.none.fl_str_mv 10000-01-01T00:00:00Z
2023-08
2023-08-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/84645
url https://hdl.handle.net/1822/84645
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciurko, D., Łaba, W., Kancelista, A., John, Ł., Gudiña, E. J., Lazar, Z., & Janek, T. (2023, August). Efficient conversion of black cumin cake from industrial waste into lipopeptide biosurfactant by Pseudomonas fluorescens. Biochemical Engineering Journal. Elsevier BV. http://doi.org/10.1016/j.bej.2023.108981
1369-703X
10.1016/j.bej.2023.108981
108981
http://www.elsevier.com/locate/issn/1369703X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132686650966016

Registros relacionados