Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART

Detalhes bibliográficos
Autor(a) principal: Sousa, Ana E.
Data de Publicação: 2000
Outros Autores: Chaves, Ana F., Doroana, Manuela, Antunes, Francisco, Victorino, Rui M. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/11129
Resumo: Copyright © 2000, Elsevier
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spelling Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAARTHIVCytokinesAntiretroviral therapyImmunological reconstitutionIntracellular cytokine detectionCopyright © 2000, ElsevierCytokine imbalances play a major role in HIV immunopathogenesis. This study analyzes simultaneously the frequency of cytokine-producing cells at the single cell level by flow cytometry and the disturbances in cytokine secretion assessed by ELISA in a cohort of asymptomatic HIV1 patients in different stages of CD4 depletion and during antiretroviral therapy (HAART). Early in the disease, there is an increased frequency of IFN-γ lymphocytes and bulk IFN-γ+ production, in parallel with a reduced proportion of IL4+ cells and IL4 secreted. The two IL4 measurements are significantly correlated. No such correlation was found for IFN-γ, which is consistent with a large variation in the amount of IFN-γ released per individual cell. Moreover, HAART was associated with a reduction to normal levels in the bulk IFN-γ secretion concomitant with a persistency of the overexpanded IFN-γ+ cell subset in the peripheral blood. This study emphasizes the importance of using a conjoint approach to assess the cytokine network in trials of antiretroviral and/or immune-based therapies to avoid missing significant effects which are possibly relevant in the clinical setting.This work was supported by grants from “Ministério da Ciência e Tecnologia–Praxis XXI” and from “Comissão Nacional de Luta Contra a SIDA–Ministério da Saúde” to RMMV. AES received a scholarship from Praxis and AFC received a scholarship from CNLCS.ElsevierRepositório da Universidade de LisboaSousa, Ana E.Chaves, Ana F.Doroana, ManuelaAntunes, FranciscoVictorino, Rui M. M.2014-06-04T15:45:37Z20002000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/11129engClinical Immunology, Vol. 97, No. 2, November, pp. 162–170, 20001521-6616http://dx.doi.org/10.1006/clim.2000.4930metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:57:04Zoai:repositorio.ul.pt:10451/11129Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:34:53.824908Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
title Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
spellingShingle Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
Sousa, Ana E.
HIV
Cytokines
Antiretroviral therapy
Immunological reconstitution
Intracellular cytokine detection
title_short Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
title_full Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
title_fullStr Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
title_full_unstemmed Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
title_sort Bulk cytokine production versus frequency of cytokine-producing cells in HIV1 infection before and during HAART
author Sousa, Ana E.
author_facet Sousa, Ana E.
Chaves, Ana F.
Doroana, Manuela
Antunes, Francisco
Victorino, Rui M. M.
author_role author
author2 Chaves, Ana F.
Doroana, Manuela
Antunes, Francisco
Victorino, Rui M. M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Sousa, Ana E.
Chaves, Ana F.
Doroana, Manuela
Antunes, Francisco
Victorino, Rui M. M.
dc.subject.por.fl_str_mv HIV
Cytokines
Antiretroviral therapy
Immunological reconstitution
Intracellular cytokine detection
topic HIV
Cytokines
Antiretroviral therapy
Immunological reconstitution
Intracellular cytokine detection
description Copyright © 2000, Elsevier
publishDate 2000
dc.date.none.fl_str_mv 2000
2000-01-01T00:00:00Z
2014-06-04T15:45:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/11129
url http://hdl.handle.net/10451/11129
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Immunology, Vol. 97, No. 2, November, pp. 162–170, 2000
1521-6616
http://dx.doi.org/10.1006/clim.2000.4930
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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