Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt

Detalhes bibliográficos
Autor(a) principal: Leandro, Tânia
Data de Publicação: 2018
Outros Autores: Rodriguez, Nuria, Rojas, Patricia, Sanz, Jose L., da Costa, Milton S., Amils, Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/107666
https://doi.org/10.1016/j.heliyon.2018.e00605
Resumo: Two deep boreholes were drilled at 320 and 620 meters below surface in the Iberian Pyritic Belt (IPB) at Peña de Hierro (Huelva, Southwestern Spain). Cores were sampled and used for the establishment of enrichment cultures with methanogenic activity. The cultivable diversity of these enrichments was accessed using different cultivation techniques and several isolates were recovered in pure culture from various depths in both boreholes. Although no archaeal isolates were obtained in pure culture, strict anaerobes and facultative anaerobic bacteria belonging to the phyla Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were isolated and identified using the 16S rRNA gene sequence. Analysis of three selected enrichment cultures by amplification of both bacterial and archaeal 16S rRNA gene followed by pyrosequencing revealed further information on the populations enriched. The archaeal sequences obtained from the methanogenic enrichment cultures belonged to the orders Methanosarcinales and Methanocellales. To best of our knowledge this is the first report of enrichment in members of the Methanocellales in a deep terrestrial subsurface ecosystem. Several bacterial populations, predominantly consisting of Firmicutes and Proteobacteria, were also enriched. The prevalent microbial populations enriched as detected by pyrosequencing analysis, as well as the bacterial isolates cultivated were affiliated with known fermentative, sulfate reducing and acetogenic bacteria or methanogenic archaea. Our results show a great diversity in the microbial communities of the IPB deep subsurface.
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spelling Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic BeltMicrobiologyEarth sciencesTwo deep boreholes were drilled at 320 and 620 meters below surface in the Iberian Pyritic Belt (IPB) at Peña de Hierro (Huelva, Southwestern Spain). Cores were sampled and used for the establishment of enrichment cultures with methanogenic activity. The cultivable diversity of these enrichments was accessed using different cultivation techniques and several isolates were recovered in pure culture from various depths in both boreholes. Although no archaeal isolates were obtained in pure culture, strict anaerobes and facultative anaerobic bacteria belonging to the phyla Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were isolated and identified using the 16S rRNA gene sequence. Analysis of three selected enrichment cultures by amplification of both bacterial and archaeal 16S rRNA gene followed by pyrosequencing revealed further information on the populations enriched. The archaeal sequences obtained from the methanogenic enrichment cultures belonged to the orders Methanosarcinales and Methanocellales. To best of our knowledge this is the first report of enrichment in members of the Methanocellales in a deep terrestrial subsurface ecosystem. Several bacterial populations, predominantly consisting of Firmicutes and Proteobacteria, were also enriched. The prevalent microbial populations enriched as detected by pyrosequencing analysis, as well as the bacterial isolates cultivated were affiliated with known fermentative, sulfate reducing and acetogenic bacteria or methanogenic archaea. Our results show a great diversity in the microbial communities of the IPB deep subsurface.Elsevier2018-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/107666http://hdl.handle.net/10316/107666https://doi.org/10.1016/j.heliyon.2018.e00605eng2405-8440Leandro, TâniaRodriguez, NuriaRojas, PatriciaSanz, Jose L.da Costa, Milton S.Amils, Ricardoinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-26T10:44:39Zoai:estudogeral.uc.pt:10316/107666Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:23:59.579231Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt
title Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt
spellingShingle Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt
Leandro, Tânia
Microbiology
Earth sciences
title_short Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt
title_full Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt
title_fullStr Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt
title_full_unstemmed Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt
title_sort Study of methanogenic enrichment cultures of rock cores from the deep subsurface of the Iberian Pyritic Belt
author Leandro, Tânia
author_facet Leandro, Tânia
Rodriguez, Nuria
Rojas, Patricia
Sanz, Jose L.
da Costa, Milton S.
Amils, Ricardo
author_role author
author2 Rodriguez, Nuria
Rojas, Patricia
Sanz, Jose L.
da Costa, Milton S.
Amils, Ricardo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Leandro, Tânia
Rodriguez, Nuria
Rojas, Patricia
Sanz, Jose L.
da Costa, Milton S.
Amils, Ricardo
dc.subject.por.fl_str_mv Microbiology
Earth sciences
topic Microbiology
Earth sciences
description Two deep boreholes were drilled at 320 and 620 meters below surface in the Iberian Pyritic Belt (IPB) at Peña de Hierro (Huelva, Southwestern Spain). Cores were sampled and used for the establishment of enrichment cultures with methanogenic activity. The cultivable diversity of these enrichments was accessed using different cultivation techniques and several isolates were recovered in pure culture from various depths in both boreholes. Although no archaeal isolates were obtained in pure culture, strict anaerobes and facultative anaerobic bacteria belonging to the phyla Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes were isolated and identified using the 16S rRNA gene sequence. Analysis of three selected enrichment cultures by amplification of both bacterial and archaeal 16S rRNA gene followed by pyrosequencing revealed further information on the populations enriched. The archaeal sequences obtained from the methanogenic enrichment cultures belonged to the orders Methanosarcinales and Methanocellales. To best of our knowledge this is the first report of enrichment in members of the Methanocellales in a deep terrestrial subsurface ecosystem. Several bacterial populations, predominantly consisting of Firmicutes and Proteobacteria, were also enriched. The prevalent microbial populations enriched as detected by pyrosequencing analysis, as well as the bacterial isolates cultivated were affiliated with known fermentative, sulfate reducing and acetogenic bacteria or methanogenic archaea. Our results show a great diversity in the microbial communities of the IPB deep subsurface.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/107666
http://hdl.handle.net/10316/107666
https://doi.org/10.1016/j.heliyon.2018.e00605
url http://hdl.handle.net/10316/107666
https://doi.org/10.1016/j.heliyon.2018.e00605
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2405-8440
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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