Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/34426 |
Resumo: | Hydrogels have been used in combination with cells for several biomedical and biotechnological applications. Nevertheless, the use of bulk hydrogels has exhibited severe limitations in diffusion of oxygen, nutrients, and metabolites. Here, a support for cell culture is reported where glucose is generated in situ by the own hydrogel degradation, allowing cell survival and function while promoting tissue growth. For this purpose, laminaran (or laminarin)-based hydrogels were fabricated, immobilizing the adequate enzymes to obtain structural platforms for 3D cell culture and providing glucose feeding for metabolic activity of cells through polysaccharide degradation. We demonstrate that tumor A549 cells and human mesenchymal stem cells (hMSCs) can use the glucose resultant from the hydrogel degradation to survive and grow in non-added glucose cell culture medium. Additionally, in vivo biocompatibility and biodegradability of laminaran-based hydrogels were explored for the first time. The self-feeding hydrogels exhibited high potential in cell survival compared to native cell-laden laminaran hydrogels over two weeks of sub-cutaneous implantation. Such bioscaffolds with enzyme-empowered degradation capacity can be applied in diverse biotechnological contexts such as tissue regeneration devices, biofactories, disease models, and cell delivery systems. |
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Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell cultureCell culture techniquesCell culture techniques, three dimensionalCell survivalHumansGlucoseHydrogelsHydrogels have been used in combination with cells for several biomedical and biotechnological applications. Nevertheless, the use of bulk hydrogels has exhibited severe limitations in diffusion of oxygen, nutrients, and metabolites. Here, a support for cell culture is reported where glucose is generated in situ by the own hydrogel degradation, allowing cell survival and function while promoting tissue growth. For this purpose, laminaran (or laminarin)-based hydrogels were fabricated, immobilizing the adequate enzymes to obtain structural platforms for 3D cell culture and providing glucose feeding for metabolic activity of cells through polysaccharide degradation. We demonstrate that tumor A549 cells and human mesenchymal stem cells (hMSCs) can use the glucose resultant from the hydrogel degradation to survive and grow in non-added glucose cell culture medium. Additionally, in vivo biocompatibility and biodegradability of laminaran-based hydrogels were explored for the first time. The self-feeding hydrogels exhibited high potential in cell survival compared to native cell-laden laminaran hydrogels over two weeks of sub-cutaneous implantation. Such bioscaffolds with enzyme-empowered degradation capacity can be applied in diverse biotechnological contexts such as tissue regeneration devices, biofactories, disease models, and cell delivery systems.Royal Society of Chemistry2023-02-01T00:00:00Z2022-02-01T00:00:00Z2022-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/34426eng10.1039/d0mh01982hZargarzadeh, MehrzadSilva, A. SofiaNunes, CláudiaCoimbra, Manuel A.Custódio, Catarina A.Mano, João F.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:06:30Zoai:ria.ua.pt:10773/34426Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:05:45.776389Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture |
title |
Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture |
spellingShingle |
Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture Zargarzadeh, Mehrzad Cell culture techniques Cell culture techniques, three dimensional Cell survival Humans Glucose Hydrogels |
title_short |
Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture |
title_full |
Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture |
title_fullStr |
Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture |
title_full_unstemmed |
Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture |
title_sort |
Self-glucose feeding hydrogels by enzyme empowered degradation for 3D cell culture |
author |
Zargarzadeh, Mehrzad |
author_facet |
Zargarzadeh, Mehrzad Silva, A. Sofia Nunes, Cláudia Coimbra, Manuel A. Custódio, Catarina A. Mano, João F. |
author_role |
author |
author2 |
Silva, A. Sofia Nunes, Cláudia Coimbra, Manuel A. Custódio, Catarina A. Mano, João F. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Zargarzadeh, Mehrzad Silva, A. Sofia Nunes, Cláudia Coimbra, Manuel A. Custódio, Catarina A. Mano, João F. |
dc.subject.por.fl_str_mv |
Cell culture techniques Cell culture techniques, three dimensional Cell survival Humans Glucose Hydrogels |
topic |
Cell culture techniques Cell culture techniques, three dimensional Cell survival Humans Glucose Hydrogels |
description |
Hydrogels have been used in combination with cells for several biomedical and biotechnological applications. Nevertheless, the use of bulk hydrogels has exhibited severe limitations in diffusion of oxygen, nutrients, and metabolites. Here, a support for cell culture is reported where glucose is generated in situ by the own hydrogel degradation, allowing cell survival and function while promoting tissue growth. For this purpose, laminaran (or laminarin)-based hydrogels were fabricated, immobilizing the adequate enzymes to obtain structural platforms for 3D cell culture and providing glucose feeding for metabolic activity of cells through polysaccharide degradation. We demonstrate that tumor A549 cells and human mesenchymal stem cells (hMSCs) can use the glucose resultant from the hydrogel degradation to survive and grow in non-added glucose cell culture medium. Additionally, in vivo biocompatibility and biodegradability of laminaran-based hydrogels were explored for the first time. The self-feeding hydrogels exhibited high potential in cell survival compared to native cell-laden laminaran hydrogels over two weeks of sub-cutaneous implantation. Such bioscaffolds with enzyme-empowered degradation capacity can be applied in diverse biotechnological contexts such as tissue regeneration devices, biofactories, disease models, and cell delivery systems. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-01T00:00:00Z 2022-02-01 2023-02-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/34426 |
url |
http://hdl.handle.net/10773/34426 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1039/d0mh01982h |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137712582688768 |