Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/10216/103421 |
Resumo: | Microalgae and cyanobacteria have received ample attention in the last few decades due to their environmental and biotechnological applications. Co-cultures of these microorganisms may present benefits particularly on wastewater bioremediation and biomass production. However, the understanding on the interactions between photosynthetic microorganisms is still in an early stage of knowledge. In this line, the aim of the present study was the evaluation of the growth dynamics of co-cultures of a cyanobacterium, Synechocystis salina, and a microalga, Pseudokirchneriella subcapitata, under low phosphate-phosphorus concentrations. Kinetic growth parameters were determined through the Monod and modified Gompertz models and evidence of allelochemical production was confirmed through metabolomic analysis of the supernatant obtained from the co-cultures using GC-MS and 1D-NMR. Kinetic growth parameters have shown that P. subcapitata was better adapted to grow under low phosphorus concentrations. Co-cultivation of these microorganisms did not influence P. subcapitata growth; however, S. salina growth was strongly inhibited. The modified Gompertz model has shown that growth inhibition of S. salina in co-cultures may be related to the activity of allelochemicals produced by P. subcapitata. This assumption was corroborated by the assessment of the antimicrobial potential of lactic acid (2-hydroxypropanoic acid), an organic acid identified in the supernatant from the co-cultures with growth inhibitory effects against S. salina. |
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Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenarioEngenharia de bioprocessos, Engenharia de projecto, Engenharia do ambienteBioprocess engineering, Project engineering, Environmental engineeringMicroalgae and cyanobacteria have received ample attention in the last few decades due to their environmental and biotechnological applications. Co-cultures of these microorganisms may present benefits particularly on wastewater bioremediation and biomass production. However, the understanding on the interactions between photosynthetic microorganisms is still in an early stage of knowledge. In this line, the aim of the present study was the evaluation of the growth dynamics of co-cultures of a cyanobacterium, Synechocystis salina, and a microalga, Pseudokirchneriella subcapitata, under low phosphate-phosphorus concentrations. Kinetic growth parameters were determined through the Monod and modified Gompertz models and evidence of allelochemical production was confirmed through metabolomic analysis of the supernatant obtained from the co-cultures using GC-MS and 1D-NMR. Kinetic growth parameters have shown that P. subcapitata was better adapted to grow under low phosphorus concentrations. Co-cultivation of these microorganisms did not influence P. subcapitata growth; however, S. salina growth was strongly inhibited. The modified Gompertz model has shown that growth inhibition of S. salina in co-cultures may be related to the activity of allelochemicals produced by P. subcapitata. This assumption was corroborated by the assessment of the antimicrobial potential of lactic acid (2-hydroxypropanoic acid), an organic acid identified in the supernatant from the co-cultures with growth inhibitory effects against S. salina.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/103421eng2046-206910.1039/c6ra07771dA. L. GonçalvesAna AbreuA. CoqueiroA. GasparF. BorgesY. ChoiJ. C. M. PiresM. Simõesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T14:24:14Zoai:repositorio-aberto.up.pt:10216/103421Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:00:30.751004Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario |
title |
Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario |
spellingShingle |
Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario A. L. Gonçalves Engenharia de bioprocessos, Engenharia de projecto, Engenharia do ambiente Bioprocess engineering, Project engineering, Environmental engineering |
title_short |
Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario |
title_full |
Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario |
title_fullStr |
Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario |
title_full_unstemmed |
Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario |
title_sort |
Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario |
author |
A. L. Gonçalves |
author_facet |
A. L. Gonçalves Ana Abreu A. Coqueiro A. Gaspar F. Borges Y. Choi J. C. M. Pires M. Simões |
author_role |
author |
author2 |
Ana Abreu A. Coqueiro A. Gaspar F. Borges Y. Choi J. C. M. Pires M. Simões |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
A. L. Gonçalves Ana Abreu A. Coqueiro A. Gaspar F. Borges Y. Choi J. C. M. Pires M. Simões |
dc.subject.por.fl_str_mv |
Engenharia de bioprocessos, Engenharia de projecto, Engenharia do ambiente Bioprocess engineering, Project engineering, Environmental engineering |
topic |
Engenharia de bioprocessos, Engenharia de projecto, Engenharia do ambiente Bioprocess engineering, Project engineering, Environmental engineering |
description |
Microalgae and cyanobacteria have received ample attention in the last few decades due to their environmental and biotechnological applications. Co-cultures of these microorganisms may present benefits particularly on wastewater bioremediation and biomass production. However, the understanding on the interactions between photosynthetic microorganisms is still in an early stage of knowledge. In this line, the aim of the present study was the evaluation of the growth dynamics of co-cultures of a cyanobacterium, Synechocystis salina, and a microalga, Pseudokirchneriella subcapitata, under low phosphate-phosphorus concentrations. Kinetic growth parameters were determined through the Monod and modified Gompertz models and evidence of allelochemical production was confirmed through metabolomic analysis of the supernatant obtained from the co-cultures using GC-MS and 1D-NMR. Kinetic growth parameters have shown that P. subcapitata was better adapted to grow under low phosphorus concentrations. Co-cultivation of these microorganisms did not influence P. subcapitata growth; however, S. salina growth was strongly inhibited. The modified Gompertz model has shown that growth inhibition of S. salina in co-cultures may be related to the activity of allelochemicals produced by P. subcapitata. This assumption was corroborated by the assessment of the antimicrobial potential of lactic acid (2-hydroxypropanoic acid), an organic acid identified in the supernatant from the co-cultures with growth inhibitory effects against S. salina. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/10216/103421 |
url |
https://hdl.handle.net/10216/103421 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2046-2069 10.1039/c6ra07771d |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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