Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario

Detalhes bibliográficos
Autor(a) principal: A. L. Gonçalves
Data de Publicação: 2016
Outros Autores: Ana Abreu, A. Coqueiro, A. Gaspar, F. Borges, Y. Choi, J. C. M. Pires, M. Simões
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/103421
Resumo: Microalgae and cyanobacteria have received ample attention in the last few decades due to their environmental and biotechnological applications. Co-cultures of these microorganisms may present benefits particularly on wastewater bioremediation and biomass production. However, the understanding on the interactions between photosynthetic microorganisms is still in an early stage of knowledge. In this line, the aim of the present study was the evaluation of the growth dynamics of co-cultures of a cyanobacterium, Synechocystis salina, and a microalga, Pseudokirchneriella subcapitata, under low phosphate-phosphorus concentrations. Kinetic growth parameters were determined through the Monod and modified Gompertz models and evidence of allelochemical production was confirmed through metabolomic analysis of the supernatant obtained from the co-cultures using GC-MS and 1D-NMR. Kinetic growth parameters have shown that P. subcapitata was better adapted to grow under low phosphorus concentrations. Co-cultivation of these microorganisms did not influence P. subcapitata growth; however, S. salina growth was strongly inhibited. The modified Gompertz model has shown that growth inhibition of S. salina in co-cultures may be related to the activity of allelochemicals produced by P. subcapitata. This assumption was corroborated by the assessment of the antimicrobial potential of lactic acid (2-hydroxypropanoic acid), an organic acid identified in the supernatant from the co-cultures with growth inhibitory effects against S. salina.
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spelling Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenarioEngenharia de bioprocessos, Engenharia de projecto, Engenharia do ambienteBioprocess engineering, Project engineering, Environmental engineeringMicroalgae and cyanobacteria have received ample attention in the last few decades due to their environmental and biotechnological applications. Co-cultures of these microorganisms may present benefits particularly on wastewater bioremediation and biomass production. However, the understanding on the interactions between photosynthetic microorganisms is still in an early stage of knowledge. In this line, the aim of the present study was the evaluation of the growth dynamics of co-cultures of a cyanobacterium, Synechocystis salina, and a microalga, Pseudokirchneriella subcapitata, under low phosphate-phosphorus concentrations. Kinetic growth parameters were determined through the Monod and modified Gompertz models and evidence of allelochemical production was confirmed through metabolomic analysis of the supernatant obtained from the co-cultures using GC-MS and 1D-NMR. Kinetic growth parameters have shown that P. subcapitata was better adapted to grow under low phosphorus concentrations. Co-cultivation of these microorganisms did not influence P. subcapitata growth; however, S. salina growth was strongly inhibited. The modified Gompertz model has shown that growth inhibition of S. salina in co-cultures may be related to the activity of allelochemicals produced by P. subcapitata. This assumption was corroborated by the assessment of the antimicrobial potential of lactic acid (2-hydroxypropanoic acid), an organic acid identified in the supernatant from the co-cultures with growth inhibitory effects against S. salina.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/103421eng2046-206910.1039/c6ra07771dA. L. GonçalvesAna AbreuA. CoqueiroA. GasparF. BorgesY. ChoiJ. C. M. PiresM. Simõesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T14:24:14Zoai:repositorio-aberto.up.pt:10216/103421Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:00:30.751004Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
title Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
spellingShingle Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
A. L. Gonçalves
Engenharia de bioprocessos, Engenharia de projecto, Engenharia do ambiente
Bioprocess engineering, Project engineering, Environmental engineering
title_short Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
title_full Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
title_fullStr Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
title_full_unstemmed Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
title_sort Co-cultivation of Synechocystis salina and Pseudokirchneriella subcapitata under varying phosphorus concentrations evidences an allelopathic competition scenario
author A. L. Gonçalves
author_facet A. L. Gonçalves
Ana Abreu
A. Coqueiro
A. Gaspar
F. Borges
Y. Choi
J. C. M. Pires
M. Simões
author_role author
author2 Ana Abreu
A. Coqueiro
A. Gaspar
F. Borges
Y. Choi
J. C. M. Pires
M. Simões
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv A. L. Gonçalves
Ana Abreu
A. Coqueiro
A. Gaspar
F. Borges
Y. Choi
J. C. M. Pires
M. Simões
dc.subject.por.fl_str_mv Engenharia de bioprocessos, Engenharia de projecto, Engenharia do ambiente
Bioprocess engineering, Project engineering, Environmental engineering
topic Engenharia de bioprocessos, Engenharia de projecto, Engenharia do ambiente
Bioprocess engineering, Project engineering, Environmental engineering
description Microalgae and cyanobacteria have received ample attention in the last few decades due to their environmental and biotechnological applications. Co-cultures of these microorganisms may present benefits particularly on wastewater bioremediation and biomass production. However, the understanding on the interactions between photosynthetic microorganisms is still in an early stage of knowledge. In this line, the aim of the present study was the evaluation of the growth dynamics of co-cultures of a cyanobacterium, Synechocystis salina, and a microalga, Pseudokirchneriella subcapitata, under low phosphate-phosphorus concentrations. Kinetic growth parameters were determined through the Monod and modified Gompertz models and evidence of allelochemical production was confirmed through metabolomic analysis of the supernatant obtained from the co-cultures using GC-MS and 1D-NMR. Kinetic growth parameters have shown that P. subcapitata was better adapted to grow under low phosphorus concentrations. Co-cultivation of these microorganisms did not influence P. subcapitata growth; however, S. salina growth was strongly inhibited. The modified Gompertz model has shown that growth inhibition of S. salina in co-cultures may be related to the activity of allelochemicals produced by P. subcapitata. This assumption was corroborated by the assessment of the antimicrobial potential of lactic acid (2-hydroxypropanoic acid), an organic acid identified in the supernatant from the co-cultures with growth inhibitory effects against S. salina.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
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dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/103421
url https://hdl.handle.net/10216/103421
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 2046-2069
10.1039/c6ra07771d
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