Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion

Detalhes bibliográficos
Autor(a) principal: Afonso, Beatriz S.
Data de Publicação: 2020
Outros Autores: Azevedo, Ana G., Gonçalves, Catarina, Amado, Isabel R., Ferreira, Eugénio C., Pastrana, Lorenzo M., Cerqueira, Miguel A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/67170
Resumo: β-carotene loaded bio-based nanoparticles (NPs) were produced by the solvent-displacement method using two polymers: zein and ethylcellulose. The production of NPs was optimised through an experimental design and characterised in terms of average size and polydispersity index. The processing conditions that allowed to obtain NPs (<100 nm) were used for β-carotene encapsulation. Then β-carotene loaded NPs were characterised in terms of zeta potential and encapsulation efficiency. Transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction analysis were performed for further morphological and chemical characterisation. In the end, a static in vitro digestion following the INFOGEST protocol was performed and the bioaccessibility of β-carotene encapsulated in both NPs was determined. Results show that the best conditions for a size-controlled production with a narrow size distribution are lower polymer concentrations and higher antisolvent concentrations. The encapsulation of β-carotene in ethylcellulose NPs resulted in nanoparticles with a mean average size of 60 ± 9 nm and encapsulation efficiency of 74 ± 2%. β-carotene loaded zein-based NPs resulted in a mean size of 83 ± 8 nm and encapsulation efficiency of 93 ± 4%. Results obtained from the in vitro digestion showed that β-carotene bioaccessibility when encapsulated in zein NPs is 37 ± 1%, which is higher than the value of 8.3 ± 0.1% obtained for the ethylcellulose NPs.
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spelling Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestionEncapsulationNanotechnologyBioactive compoundsZeinEthylcelluloseBiopolymersScience & Technologyβ-carotene loaded bio-based nanoparticles (NPs) were produced by the solvent-displacement method using two polymers: zein and ethylcellulose. The production of NPs was optimised through an experimental design and characterised in terms of average size and polydispersity index. The processing conditions that allowed to obtain NPs (<100 nm) were used for β-carotene encapsulation. Then β-carotene loaded NPs were characterised in terms of zeta potential and encapsulation efficiency. Transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction analysis were performed for further morphological and chemical characterisation. In the end, a static in vitro digestion following the INFOGEST protocol was performed and the bioaccessibility of β-carotene encapsulated in both NPs was determined. Results show that the best conditions for a size-controlled production with a narrow size distribution are lower polymer concentrations and higher antisolvent concentrations. The encapsulation of β-carotene in ethylcellulose NPs resulted in nanoparticles with a mean average size of 60 ± 9 nm and encapsulation efficiency of 74 ± 2%. β-carotene loaded zein-based NPs resulted in a mean size of 83 ± 8 nm and encapsulation efficiency of 93 ± 4%. Results obtained from the in vitro digestion showed that β-carotene bioaccessibility when encapsulated in zein NPs is 37 ± 1%, which is higher than the value of 8.3 ± 0.1% obtained for the ethylcellulose NPs.This research was funded by “MobFood—Mobilizing scientific and technological knowledge in response to the challenges of the agri-food market” (POCI-01-0247-FEDER-024524; LISBOA-01-0247-FEDER024524), by “MobFood” Consortium, and financed by European Regional Development Fund (ERDF), through the Incentive System to Research and Technological development, within the Portugal2020 Competitiveness and Internationalization Operational Program. The research also received funding from the European Union’s H2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement N 778388 (H2020MSCA-RISE-2017 project Food for Diabetes and Cognition (FODIAC), and grant agreement N 713640 (MSCA2015-COFUND-FP), and MICRODIGEST project (grant agreement 037716) co-funded by FCT and ERDF through COMPETE2020.info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoAfonso, Beatriz S.Azevedo, Ana G.Gonçalves, CatarinaAmado, Isabel R.Ferreira, Eugénio C.Pastrana, Lorenzo M.Cerqueira, Miguel A.2020-09-302020-09-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67170engAfonso, Beatriz S.; Azevedo, Ana G.; Gonçalves, Catarina; Amado, Isabel R.; Ferreira, Eugénio C.; Pastrana, Lorenzo M.; Cerqueira, Miguel A., Bio-based nanoparticles as a carrier of -carotene: production, characterisation and in vitro gastrointestinal digestion. Molecules, 25(19), 4497, 20201420-304910.3390/molecules2519449733008004https://www.mdpi.com/journal/moleculesinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:18Zoai:repositorium.sdum.uminho.pt:1822/67170Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:04:12.726044Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion
title Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion
spellingShingle Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion
Afonso, Beatriz S.
Encapsulation
Nanotechnology
Bioactive compounds
Zein
Ethylcellulose
Biopolymers
Science & Technology
title_short Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion
title_full Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion
title_fullStr Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion
title_full_unstemmed Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion
title_sort Bio-based nanoparticles as a carrier of β-carotene: production, characterisation and in vitro gastrointestinal digestion
author Afonso, Beatriz S.
author_facet Afonso, Beatriz S.
Azevedo, Ana G.
Gonçalves, Catarina
Amado, Isabel R.
Ferreira, Eugénio C.
Pastrana, Lorenzo M.
Cerqueira, Miguel A.
author_role author
author2 Azevedo, Ana G.
Gonçalves, Catarina
Amado, Isabel R.
Ferreira, Eugénio C.
Pastrana, Lorenzo M.
Cerqueira, Miguel A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Afonso, Beatriz S.
Azevedo, Ana G.
Gonçalves, Catarina
Amado, Isabel R.
Ferreira, Eugénio C.
Pastrana, Lorenzo M.
Cerqueira, Miguel A.
dc.subject.por.fl_str_mv Encapsulation
Nanotechnology
Bioactive compounds
Zein
Ethylcellulose
Biopolymers
Science & Technology
topic Encapsulation
Nanotechnology
Bioactive compounds
Zein
Ethylcellulose
Biopolymers
Science & Technology
description β-carotene loaded bio-based nanoparticles (NPs) were produced by the solvent-displacement method using two polymers: zein and ethylcellulose. The production of NPs was optimised through an experimental design and characterised in terms of average size and polydispersity index. The processing conditions that allowed to obtain NPs (<100 nm) were used for β-carotene encapsulation. Then β-carotene loaded NPs were characterised in terms of zeta potential and encapsulation efficiency. Transmission electron microscopy, Fourier transform infrared spectroscopy and X-ray diffraction analysis were performed for further morphological and chemical characterisation. In the end, a static in vitro digestion following the INFOGEST protocol was performed and the bioaccessibility of β-carotene encapsulated in both NPs was determined. Results show that the best conditions for a size-controlled production with a narrow size distribution are lower polymer concentrations and higher antisolvent concentrations. The encapsulation of β-carotene in ethylcellulose NPs resulted in nanoparticles with a mean average size of 60 ± 9 nm and encapsulation efficiency of 74 ± 2%. β-carotene loaded zein-based NPs resulted in a mean size of 83 ± 8 nm and encapsulation efficiency of 93 ± 4%. Results obtained from the in vitro digestion showed that β-carotene bioaccessibility when encapsulated in zein NPs is 37 ± 1%, which is higher than the value of 8.3 ± 0.1% obtained for the ethylcellulose NPs.
publishDate 2020
dc.date.none.fl_str_mv 2020-09-30
2020-09-30T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/67170
url http://hdl.handle.net/1822/67170
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Afonso, Beatriz S.; Azevedo, Ana G.; Gonçalves, Catarina; Amado, Isabel R.; Ferreira, Eugénio C.; Pastrana, Lorenzo M.; Cerqueira, Miguel A., Bio-based nanoparticles as a carrier of -carotene: production, characterisation and in vitro gastrointestinal digestion. Molecules, 25(19), 4497, 2020
1420-3049
10.3390/molecules25194497
33008004
https://www.mdpi.com/journal/molecules
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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