Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/51147 |
Resumo: | The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fpls.2016. 01150 |
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Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berriesFlavonoidsFruit qualityGrape berryKaolinMetabolic changesPhenylpropanoidsSecondary metabolitesStress mitigationThe Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fpls.2016. 01150Drought, elevated air temperature, and high evaporative demand are increasingly frequent during summer in grape growing areas like the Mediterranean basin, limiting grapevine productivity and berry quality. The foliar exogenous application of kaolin, a radiation-reflecting inert mineral, has proven effective in mitigating the negative impacts of these abiotic stresses in grapevine and other fruit crops, however, little is known about its influence on the composition of the grape berry and on key molecular mechanisms and metabolic pathways notably important for grape berry quality parameters. Here, we performed a thorough molecular and biochemical analysis to assess how foliar application of kaolin influences major secondary metabolism pathways associated with berry quality-traits, leading to biosynthesis of phenolics and anthocyanins, with a focus on the phenylpropanoid, flavonoid (both flavonol- and anthocyanin-biosynthetic) and stilbenoid pathways. In grape berries from different ripening stages, targeted transcriptional analysis by qPCR revealed that several genes involved in these pathways—VvPAL1, VvC4H1, VvSTSs, VvCHS1, VvFLS1, VvDFR, and VvUFGT—were more expressed in response to the foliar kaolin treatment, particularly in the latter maturation phases. In agreement, enzymatic activities of phenylalanine ammonia lyase (PAL), flavonol synthase (FLS), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) were about two-fold higher in mature or fully mature berries from kaolin-treated plants, suggesting regulation also at a transcriptional level. The expression of the glutathione S-transferase VvGST4, and of the tonoplast anthocyanin transporters VvMATE1 and VvABCC1 were also all significantly increased at véraison and in mature berries, thus, when anthocyanins start to accumulate in the vacuole, in agreement with previously observed higher total concentrations of phenolics and anthocyanins in berries from kaolin-treated plaThe work was supported by European Union Funds (FEDER/COMPETE-Operational Competitiveness Programme —INNOVINE—ref. 311775, Enoexcel—Norte—07-0124FEDER-000032 and INTERACT - NORTE-01-0145-FEDER000017 - Linha VitalityWine - ON 0013), and by Portuguese national funds (FCT-Portuguese Foundation for Science and Technology) under the project FCOMP-01-0124FEDER-022692. AC was supported by Enoexcel—Norte— 07-0124-FEDER-000032 and INTERACT - NORTE-010145-FEDER-000017.This work also benefited from the networking activities within the European funded COST ACTION FA1106 “QualityFruit.”info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoConde, ArturPimentel, DianaNeves, AndreiaDinis, Lia TâniaBernardo, SaraCorreia, Carlos M.Gerós, H.Moutinho-Pereira, José20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51147eng10.3389/fpls.2016.01150info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:00Zoai:repositorium.sdum.uminho.pt:1822/51147Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:33.113666Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries |
| title |
Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries |
| spellingShingle |
Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries Conde, Artur Flavonoids Fruit quality Grape berry Kaolin Metabolic changes Phenylpropanoids Secondary metabolites Stress mitigation |
| title_short |
Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries |
| title_full |
Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries |
| title_fullStr |
Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries |
| title_full_unstemmed |
Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries |
| title_sort |
Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries |
| author |
Conde, Artur |
| author_facet |
Conde, Artur Pimentel, Diana Neves, Andreia Dinis, Lia Tânia Bernardo, Sara Correia, Carlos M. Gerós, H. Moutinho-Pereira, José |
| author_role |
author |
| author2 |
Pimentel, Diana Neves, Andreia Dinis, Lia Tânia Bernardo, Sara Correia, Carlos M. Gerós, H. Moutinho-Pereira, José |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Conde, Artur Pimentel, Diana Neves, Andreia Dinis, Lia Tânia Bernardo, Sara Correia, Carlos M. Gerós, H. Moutinho-Pereira, José |
| dc.subject.por.fl_str_mv |
Flavonoids Fruit quality Grape berry Kaolin Metabolic changes Phenylpropanoids Secondary metabolites Stress mitigation |
| topic |
Flavonoids Fruit quality Grape berry Kaolin Metabolic changes Phenylpropanoids Secondary metabolites Stress mitigation |
| description |
The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fpls.2016. 01150 |
| publishDate |
2016 |
| dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/51147 |
| url |
http://hdl.handle.net/1822/51147 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.3389/fpls.2016.01150 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
| publisher.none.fl_str_mv |
Frontiers Media |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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