Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries

Detalhes bibliográficos
Autor(a) principal: Conde, Artur
Data de Publicação: 2016
Outros Autores: Pimentel, Diana, Neves, Andreia, Dinis, Lia Tânia, Bernardo, Sara, Correia, Carlos M., Gerós, H., Moutinho-Pereira, José
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/51147
Resumo: The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fpls.2016. 01150
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spelling Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berriesFlavonoidsFruit qualityGrape berryKaolinMetabolic changesPhenylpropanoidsSecondary metabolitesStress mitigationThe Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fpls.2016. 01150Drought, elevated air temperature, and high evaporative demand are increasingly frequent during summer in grape growing areas like the Mediterranean basin, limiting grapevine productivity and berry quality. The foliar exogenous application of kaolin, a radiation-reflecting inert mineral, has proven effective in mitigating the negative impacts of these abiotic stresses in grapevine and other fruit crops, however, little is known about its influence on the composition of the grape berry and on key molecular mechanisms and metabolic pathways notably important for grape berry quality parameters. Here, we performed a thorough molecular and biochemical analysis to assess how foliar application of kaolin influences major secondary metabolism pathways associated with berry quality-traits, leading to biosynthesis of phenolics and anthocyanins, with a focus on the phenylpropanoid, flavonoid (both flavonol- and anthocyanin-biosynthetic) and stilbenoid pathways. In grape berries from different ripening stages, targeted transcriptional analysis by qPCR revealed that several genes involved in these pathways—VvPAL1, VvC4H1, VvSTSs, VvCHS1, VvFLS1, VvDFR, and VvUFGT—were more expressed in response to the foliar kaolin treatment, particularly in the latter maturation phases. In agreement, enzymatic activities of phenylalanine ammonia lyase (PAL), flavonol synthase (FLS), and UDP-glucose:flavonoid 3-O-glucosyltransferase (UFGT) were about two-fold higher in mature or fully mature berries from kaolin-treated plants, suggesting regulation also at a transcriptional level. The expression of the glutathione S-transferase VvGST4, and of the tonoplast anthocyanin transporters VvMATE1 and VvABCC1 were also all significantly increased at véraison and in mature berries, thus, when anthocyanins start to accumulate in the vacuole, in agreement with previously observed higher total concentrations of phenolics and anthocyanins in berries from kaolin-treated plaThe work was supported by European Union Funds (FEDER/COMPETE-Operational Competitiveness Programme —INNOVINE—ref. 311775, Enoexcel—Norte—07-0124FEDER-000032 and INTERACT - NORTE-01-0145-FEDER000017 - Linha VitalityWine - ON 0013), and by Portuguese national funds (FCT-Portuguese Foundation for Science and Technology) under the project FCOMP-01-0124FEDER-022692. AC was supported by Enoexcel—Norte— 07-0124-FEDER-000032 and INTERACT - NORTE-010145-FEDER-000017.This work also benefited from the networking activities within the European funded COST ACTION FA1106 “QualityFruit.”info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoConde, ArturPimentel, DianaNeves, AndreiaDinis, Lia TâniaBernardo, SaraCorreia, Carlos M.Gerós, H.Moutinho-Pereira, José20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51147eng10.3389/fpls.2016.01150info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:30:10Zoai:repositorium.sdum.uminho.pt:1822/51147Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:30:10Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
title Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
spellingShingle Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
Conde, Artur
Flavonoids
Fruit quality
Grape berry
Kaolin
Metabolic changes
Phenylpropanoids
Secondary metabolites
Stress mitigation
title_short Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
title_full Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
title_fullStr Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
title_full_unstemmed Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
title_sort Kaolin foliar application has a stimulatory effect on phenylpropanoid and flavonoid pathways in grape berries
author Conde, Artur
author_facet Conde, Artur
Pimentel, Diana
Neves, Andreia
Dinis, Lia Tânia
Bernardo, Sara
Correia, Carlos M.
Gerós, H.
Moutinho-Pereira, José
author_role author
author2 Pimentel, Diana
Neves, Andreia
Dinis, Lia Tânia
Bernardo, Sara
Correia, Carlos M.
Gerós, H.
Moutinho-Pereira, José
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Conde, Artur
Pimentel, Diana
Neves, Andreia
Dinis, Lia Tânia
Bernardo, Sara
Correia, Carlos M.
Gerós, H.
Moutinho-Pereira, José
dc.subject.por.fl_str_mv Flavonoids
Fruit quality
Grape berry
Kaolin
Metabolic changes
Phenylpropanoids
Secondary metabolites
Stress mitigation
topic Flavonoids
Fruit quality
Grape berry
Kaolin
Metabolic changes
Phenylpropanoids
Secondary metabolites
Stress mitigation
description The Supplementary Material for this article can be found online at: http://journal.frontiersin.org/article/10.3389/fpls.2016. 01150
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/51147
url http://hdl.handle.net/1822/51147
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.3389/fpls.2016.01150
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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