Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration

Detalhes bibliográficos
Autor(a) principal: Alves, Ana L.
Data de Publicação: 2023
Outros Autores: Carvalho, Ana C., Machado, Inês, Diogo, Gabriela S., Fernandes, Emanuel M., Castro, Vânia Isabel Baptista, Pires, Ricardo A., Vázquez, José A., Pérez-Martín, Ricardo I., Alaminos, Miguel, Reis, R. L., Silva, Tiago H.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/84802
Resumo: Corneal pathologies from infectious or noninfectious origin have a significant impact on the daily lives of millions of people worldwide. Despite the risk of organ rejection or infection, corneal transplantation is currently the only effective treatment. Finding safe and innovative strategies is the main goal of tissue-engineering-based approaches. In this study, the potential of gelatin methacryloyl (GelMA) hydrogels produced from marine-derived gelatin and loaded with ascorbic acid (as an enhancer of the biological activity of cells) was evaluated for corneal stromal applications. Marine GelMA was synthesized with a methacrylation degree of 75%, enabling effective photocrosslinking, and hydrogels with or without ascorbic acid were produced, encompassing human keratocytes. All the produced formulations exhibited excellent optical and swelling properties with easy handling as well as structural stability and adequate degradation rates that may allow proper extracellular matrix remodeling by corneal stromal cells. Formulations loaded with 0.5 mg/mL of ascorbic acid enhanced the biological performance of keratocytes and induced collagen production. These results suggest that, in addition to marine-derived gelatin being suitable for the synthesis of GelMA, the hydrogels produced are promising biomaterials for corneal regeneration applications.
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spelling Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regenerationMarine biomaterialsGelatinCodfishGelMAKeratocytesCorneaScience & TechnologyCorneal pathologies from infectious or noninfectious origin have a significant impact on the daily lives of millions of people worldwide. Despite the risk of organ rejection or infection, corneal transplantation is currently the only effective treatment. Finding safe and innovative strategies is the main goal of tissue-engineering-based approaches. In this study, the potential of gelatin methacryloyl (GelMA) hydrogels produced from marine-derived gelatin and loaded with ascorbic acid (as an enhancer of the biological activity of cells) was evaluated for corneal stromal applications. Marine GelMA was synthesized with a methacrylation degree of 75%, enabling effective photocrosslinking, and hydrogels with or without ascorbic acid were produced, encompassing human keratocytes. All the produced formulations exhibited excellent optical and swelling properties with easy handling as well as structural stability and adequate degradation rates that may allow proper extracellular matrix remodeling by corneal stromal cells. Formulations loaded with 0.5 mg/mL of ascorbic acid enhanced the biological performance of keratocytes and induced collagen production. These results suggest that, in addition to marine-derived gelatin being suitable for the synthesis of GelMA, the hydrogels produced are promising biomaterials for corneal regeneration applications.This research has received funds from the European Regional Development Fund (ERDF) under the scope of INTERREG Atlantic Area Programme through the project BLUEHUMAN (EAPA_151/2016), under the scope of INTERREG España–Portugal Programme through the project CVMar+I (0302_CVMAR_I_1_P), and under the scope of NORTE2020 through the project ATLANTIDA (Norte-01-0145-FEDER-000040) and the R&D Infrastructure TERM RES-Hub PINFRA/ 22190/2016 (Norte-01-0145-FEDER-022190), also funded by the Portuguese Foundation for Science and Technology (FCT). Financial support from FCT is also acknowledged for the PhD scholarship of Ana. L. Alves (PD/BD/127995/2016) under Doctoral Programme Do*Mar and the investigator contract of Ricardo Pires from institutional CEEC program (CEECINST/00077/2018). José Antonio Vázquez and Ricardo I. Pérez-Martín want to thank Xunta de Galicia (Grupos de Potential Crecimiento, IN607B 2021/11) for their financial support.Multidisciplinary Digital Publishing Institute (MDPI)Universidade do MinhoAlves, Ana L.Carvalho, Ana C.Machado, InêsDiogo, Gabriela S.Fernandes, Emanuel M.Castro, Vânia Isabel BaptistaPires, Ricardo A.Vázquez, José A.Pérez-Martín, Ricardo I.Alaminos, MiguelReis, R. L.Silva, Tiago H.2023-01-042023-01-04T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/84802engAlves, A.L.; Carvalho, A.C.; Machado, I.; Diogo, G.S.; Fernandes, E.M.; Castro, V.I.B.; Pires, R.A.; Vázquez, J.A.; Pérez-Martín, R.I.; Alaminos, M.; Reis, R.L.; Silva, T.H. Cell-Laden Marine Gelatin Methacryloyl Hydrogels Enriched with Ascorbic Acid for Corneal Stroma Regeneration. Bioengineering 2023, 10, 62. https://doi.org/10.3390/bioengineering100100622306-535410.3390/bioengineering1001006262https://www.mdpi.com/2306-5354/10/1/62info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-23T01:29:45Zoai:repositorium.sdum.uminho.pt:1822/84802Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:07:38.822798Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration
title Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration
spellingShingle Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration
Alves, Ana L.
Marine biomaterials
Gelatin
Codfish
GelMA
Keratocytes
Cornea
Science & Technology
title_short Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration
title_full Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration
title_fullStr Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration
title_full_unstemmed Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration
title_sort Cell-laden marine gelatin methacryloyl hydrogels enriched with ascorbic acid for corneal stroma regeneration
author Alves, Ana L.
author_facet Alves, Ana L.
Carvalho, Ana C.
Machado, Inês
Diogo, Gabriela S.
Fernandes, Emanuel M.
Castro, Vânia Isabel Baptista
Pires, Ricardo A.
Vázquez, José A.
Pérez-Martín, Ricardo I.
Alaminos, Miguel
Reis, R. L.
Silva, Tiago H.
author_role author
author2 Carvalho, Ana C.
Machado, Inês
Diogo, Gabriela S.
Fernandes, Emanuel M.
Castro, Vânia Isabel Baptista
Pires, Ricardo A.
Vázquez, José A.
Pérez-Martín, Ricardo I.
Alaminos, Miguel
Reis, R. L.
Silva, Tiago H.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Alves, Ana L.
Carvalho, Ana C.
Machado, Inês
Diogo, Gabriela S.
Fernandes, Emanuel M.
Castro, Vânia Isabel Baptista
Pires, Ricardo A.
Vázquez, José A.
Pérez-Martín, Ricardo I.
Alaminos, Miguel
Reis, R. L.
Silva, Tiago H.
dc.subject.por.fl_str_mv Marine biomaterials
Gelatin
Codfish
GelMA
Keratocytes
Cornea
Science & Technology
topic Marine biomaterials
Gelatin
Codfish
GelMA
Keratocytes
Cornea
Science & Technology
description Corneal pathologies from infectious or noninfectious origin have a significant impact on the daily lives of millions of people worldwide. Despite the risk of organ rejection or infection, corneal transplantation is currently the only effective treatment. Finding safe and innovative strategies is the main goal of tissue-engineering-based approaches. In this study, the potential of gelatin methacryloyl (GelMA) hydrogels produced from marine-derived gelatin and loaded with ascorbic acid (as an enhancer of the biological activity of cells) was evaluated for corneal stromal applications. Marine GelMA was synthesized with a methacrylation degree of 75%, enabling effective photocrosslinking, and hydrogels with or without ascorbic acid were produced, encompassing human keratocytes. All the produced formulations exhibited excellent optical and swelling properties with easy handling as well as structural stability and adequate degradation rates that may allow proper extracellular matrix remodeling by corneal stromal cells. Formulations loaded with 0.5 mg/mL of ascorbic acid enhanced the biological performance of keratocytes and induced collagen production. These results suggest that, in addition to marine-derived gelatin being suitable for the synthesis of GelMA, the hydrogels produced are promising biomaterials for corneal regeneration applications.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-04
2023-01-04T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/84802
url https://hdl.handle.net/1822/84802
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Alves, A.L.; Carvalho, A.C.; Machado, I.; Diogo, G.S.; Fernandes, E.M.; Castro, V.I.B.; Pires, R.A.; Vázquez, J.A.; Pérez-Martín, R.I.; Alaminos, M.; Reis, R.L.; Silva, T.H. Cell-Laden Marine Gelatin Methacryloyl Hydrogels Enriched with Ascorbic Acid for Corneal Stroma Regeneration. Bioengineering 2023, 10, 62. https://doi.org/10.3390/bioengineering10010062
2306-5354
10.3390/bioengineering10010062
62
https://www.mdpi.com/2306-5354/10/1/62
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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