Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/108265 https://doi.org/10.3390/md15030062 |
Resumo: | The lipidome of the red seaweed Gracilaria sp., cultivated on land-based integrated multitrophic aquaculture (IMTA) system, was assessed for the first time using hydrophilic interaction liquid chromatography-mass spectrometry and tandem mass spectrometry (HILIC-MS and MS/MS). One hundred and forty-seven molecular species were identified in the lipidome of the Gracilaria genus and distributed between the glycolipids classes monogalactosyl diacylglyceride (MGDG), digalactosyl diacylglyceride (DGDG), sulfoquinovosyl monoacylglyceride (SQMG), sulfoquinovosyl diacylglyceride (SQDG), the phospholipids phosphatidylcholine (PC), lyso-PC, phosphatidylglycerol (PG), lyso-PG, phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatic acid (PA), inositolphosphoceramide (IPC), and betaine lipids monoacylglyceryl- and diacylglyceryl-N,N,N-trimethyl homoserine (MGTS and DGTS). Antiproliferative and anti-inflammatory effects promoted by lipid extract of Gracilaria sp. were evaluated by monitoring cell viability in human cancer lines and by using murine macrophages, respectively. The lipid extract decreased cell viability of human T-47D breast cancer cells and of 5637 human bladder cancer cells (estimated half-maximal inhibitory concentration (IC50) of 12.2 μg/mL and 12.9 μg/mL, respectively) and inhibited the production of nitric oxide (NO) evoked by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) on the macrophage cell line RAW 264.7 (35% inhibition at a concentration of 100 μg/mL). These findings contribute to increase the ranking in the value-chain of Gracilaria sp. biomass cultivated under controlled conditions on IMTA systems. |
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Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activityglycolipidsphospholipidsbetaine lipidsseaweedsbioactivitymass spectrometryhydrophilic interaction liquid chromatography–electrospray ionization–mass spectrometry HILIC–ESI–MSAnimalsAnti-Inflammatory AgentsBiomassBreast NeoplasmsCell LineCell Line, TumorCell ProliferationCell SurvivalChromatography, LiquidGlycolipidsGracilariaHumansLipidsMacrophagesMiceNitric OxideSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryToll-Like Receptor 4Urinary Bladder NeoplasmsThe lipidome of the red seaweed Gracilaria sp., cultivated on land-based integrated multitrophic aquaculture (IMTA) system, was assessed for the first time using hydrophilic interaction liquid chromatography-mass spectrometry and tandem mass spectrometry (HILIC-MS and MS/MS). One hundred and forty-seven molecular species were identified in the lipidome of the Gracilaria genus and distributed between the glycolipids classes monogalactosyl diacylglyceride (MGDG), digalactosyl diacylglyceride (DGDG), sulfoquinovosyl monoacylglyceride (SQMG), sulfoquinovosyl diacylglyceride (SQDG), the phospholipids phosphatidylcholine (PC), lyso-PC, phosphatidylglycerol (PG), lyso-PG, phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatic acid (PA), inositolphosphoceramide (IPC), and betaine lipids monoacylglyceryl- and diacylglyceryl-N,N,N-trimethyl homoserine (MGTS and DGTS). Antiproliferative and anti-inflammatory effects promoted by lipid extract of Gracilaria sp. were evaluated by monitoring cell viability in human cancer lines and by using murine macrophages, respectively. The lipid extract decreased cell viability of human T-47D breast cancer cells and of 5637 human bladder cancer cells (estimated half-maximal inhibitory concentration (IC50) of 12.2 μg/mL and 12.9 μg/mL, respectively) and inhibited the production of nitric oxide (NO) evoked by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) on the macrophage cell line RAW 264.7 (35% inhibition at a concentration of 100 μg/mL). These findings contribute to increase the ranking in the value-chain of Gracilaria sp. biomass cultivated under controlled conditions on IMTA systems.MDPI2017-03-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/108265http://hdl.handle.net/10316/108265https://doi.org/10.3390/md15030062eng1660-3397Costa, Elisabete daMelo, TâniaMoreira, Ana S. P.Bernardo, CarinaHelguero, LuisaFerreira, IsabelCruz, Maria TeresaRego, Andreia M.Domingues, PedroCalado, RicardoAbreu, Maria H.Domingues, Maria Rosárioinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-21T11:36:29Zoai:estudogeral.uc.pt:10316/108265Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:24:34.351621Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity |
title |
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity |
spellingShingle |
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity Costa, Elisabete da glycolipids phospholipids betaine lipids seaweeds bioactivity mass spectrometry hydrophilic interaction liquid chromatography–electrospray ionization–mass spectrometry HILIC–ESI–MS Animals Anti-Inflammatory Agents Biomass Breast Neoplasms Cell Line Cell Line, Tumor Cell Proliferation Cell Survival Chromatography, Liquid Glycolipids Gracilaria Humans Lipids Macrophages Mice Nitric Oxide Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Toll-Like Receptor 4 Urinary Bladder Neoplasms |
title_short |
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity |
title_full |
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity |
title_fullStr |
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity |
title_full_unstemmed |
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity |
title_sort |
Valorization of Lipids from Gracilaria sp. through Lipidomics and Decoding of Antiproliferative and Anti-Inflammatory Activity |
author |
Costa, Elisabete da |
author_facet |
Costa, Elisabete da Melo, Tânia Moreira, Ana S. P. Bernardo, Carina Helguero, Luisa Ferreira, Isabel Cruz, Maria Teresa Rego, Andreia M. Domingues, Pedro Calado, Ricardo Abreu, Maria H. Domingues, Maria Rosário |
author_role |
author |
author2 |
Melo, Tânia Moreira, Ana S. P. Bernardo, Carina Helguero, Luisa Ferreira, Isabel Cruz, Maria Teresa Rego, Andreia M. Domingues, Pedro Calado, Ricardo Abreu, Maria H. Domingues, Maria Rosário |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Costa, Elisabete da Melo, Tânia Moreira, Ana S. P. Bernardo, Carina Helguero, Luisa Ferreira, Isabel Cruz, Maria Teresa Rego, Andreia M. Domingues, Pedro Calado, Ricardo Abreu, Maria H. Domingues, Maria Rosário |
dc.subject.por.fl_str_mv |
glycolipids phospholipids betaine lipids seaweeds bioactivity mass spectrometry hydrophilic interaction liquid chromatography–electrospray ionization–mass spectrometry HILIC–ESI–MS Animals Anti-Inflammatory Agents Biomass Breast Neoplasms Cell Line Cell Line, Tumor Cell Proliferation Cell Survival Chromatography, Liquid Glycolipids Gracilaria Humans Lipids Macrophages Mice Nitric Oxide Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Toll-Like Receptor 4 Urinary Bladder Neoplasms |
topic |
glycolipids phospholipids betaine lipids seaweeds bioactivity mass spectrometry hydrophilic interaction liquid chromatography–electrospray ionization–mass spectrometry HILIC–ESI–MS Animals Anti-Inflammatory Agents Biomass Breast Neoplasms Cell Line Cell Line, Tumor Cell Proliferation Cell Survival Chromatography, Liquid Glycolipids Gracilaria Humans Lipids Macrophages Mice Nitric Oxide Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Toll-Like Receptor 4 Urinary Bladder Neoplasms |
description |
The lipidome of the red seaweed Gracilaria sp., cultivated on land-based integrated multitrophic aquaculture (IMTA) system, was assessed for the first time using hydrophilic interaction liquid chromatography-mass spectrometry and tandem mass spectrometry (HILIC-MS and MS/MS). One hundred and forty-seven molecular species were identified in the lipidome of the Gracilaria genus and distributed between the glycolipids classes monogalactosyl diacylglyceride (MGDG), digalactosyl diacylglyceride (DGDG), sulfoquinovosyl monoacylglyceride (SQMG), sulfoquinovosyl diacylglyceride (SQDG), the phospholipids phosphatidylcholine (PC), lyso-PC, phosphatidylglycerol (PG), lyso-PG, phosphatidylinositol (PI), phosphatidylethanolamine (PE), phosphatic acid (PA), inositolphosphoceramide (IPC), and betaine lipids monoacylglyceryl- and diacylglyceryl-N,N,N-trimethyl homoserine (MGTS and DGTS). Antiproliferative and anti-inflammatory effects promoted by lipid extract of Gracilaria sp. were evaluated by monitoring cell viability in human cancer lines and by using murine macrophages, respectively. The lipid extract decreased cell viability of human T-47D breast cancer cells and of 5637 human bladder cancer cells (estimated half-maximal inhibitory concentration (IC50) of 12.2 μg/mL and 12.9 μg/mL, respectively) and inhibited the production of nitric oxide (NO) evoked by the Toll-like receptor 4 agonist lipopolysaccharide (LPS) on the macrophage cell line RAW 264.7 (35% inhibition at a concentration of 100 μg/mL). These findings contribute to increase the ranking in the value-chain of Gracilaria sp. biomass cultivated under controlled conditions on IMTA systems. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/108265 http://hdl.handle.net/10316/108265 https://doi.org/10.3390/md15030062 |
url |
http://hdl.handle.net/10316/108265 https://doi.org/10.3390/md15030062 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1660-3397 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1817553079191470080 |