Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Pedro L.
Data de Publicação: 2014
Outros Autores: Rodrigues, Nuno F., Duque, Duarte, Granja, Sara Costa, Pinto, Jorge Correia, Vilaça, João L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/30426
Resumo: Article ID 820214
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spelling Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explantsScience & TechnologyArticle ID 820214Background. Regulating mechanisms of branching morphogenesis of fetal lung rat explants have been an essential tool for molecular research. This work presents a new methodology to accurately quantify the epithelial, outer contour, and peripheral airway buds of lung explants during cellular development from microscopic images. Methods. The outer contour was defined using an adaptive and multiscale threshold algorithm whose level was automatically calculated based on an entropy maximization criterion. The inner lung epithelium was defined by a clustering procedure that groups small image regions according to the minimum description length principle and local statistical properties. Finally, the number of peripheral buds was counted as the skeleton branched ends from a skeletonized image of the lung inner epithelia. Results. The time for lung branching morphometric analysis was reduced in 98% in contrast to the manual method. Best results were obtained in the first two days of cellular development, with lesser standard deviations. Nonsignificant differences were found between the automatic and manual results in all culture days. Conclusions. The proposed method introduces a series of advantages related to its intuitive use and accuracy, making the technique suitable to images with different lighting characteristics and allowing a reliable comparison between different researchers.The authors acknowledge Foundation for Science and Technology (FCT), Portugal, for the fellowship with the references: SFRH/BD/74276/2010 and SFRH/BPD/46851/2008.Hindawi Publishing CorporationUniversidade do MinhoRodrigues, Pedro L.Rodrigues, Nuno F.Duque, DuarteGranja, Sara CostaPinto, Jorge CorreiaVilaça, João L.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/30426eng1748-670X10.1155/2014/82021425250057http://www.hindawi.com/journals/cmmm/2014/820214/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:21Zoai:repositorium.sdum.uminho.pt:1822/30426Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:38.694990Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
title Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
spellingShingle Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
Rodrigues, Pedro L.
Science & Technology
title_short Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
title_full Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
title_fullStr Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
title_full_unstemmed Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
title_sort Automated image analysis of lung branching morphogenesis from microscopic images of fetal rat explants
author Rodrigues, Pedro L.
author_facet Rodrigues, Pedro L.
Rodrigues, Nuno F.
Duque, Duarte
Granja, Sara Costa
Pinto, Jorge Correia
Vilaça, João L.
author_role author
author2 Rodrigues, Nuno F.
Duque, Duarte
Granja, Sara Costa
Pinto, Jorge Correia
Vilaça, João L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, Pedro L.
Rodrigues, Nuno F.
Duque, Duarte
Granja, Sara Costa
Pinto, Jorge Correia
Vilaça, João L.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description Article ID 820214
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/30426
url http://hdl.handle.net/1822/30426
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1748-670X
10.1155/2014/820214
25250057
http://www.hindawi.com/journals/cmmm/2014/820214/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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