Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/117154 |
Resumo: | PTDC/AAG-TEC/5400/2014 POCI-01-0145-FEDER-016637 POCI-010145-FEDER-007688 UID/CTM/50025/2019 SFRH/BD/115173/2016 SFRH/BPD/97891/2013 |
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oai:run.unl.pt:10362/117154 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-AmyloidChemistry(all)Chemical Engineering(all)PTDC/AAG-TEC/5400/2014 POCI-01-0145-FEDER-016637 POCI-010145-FEDER-007688 UID/CTM/50025/2019 SFRH/BD/115173/2016 SFRH/BPD/97891/2013Alzheimer's disease (AD) is one of the most common forms of dementia affecting millions of people worldwide. Currently, an easy and effective form of diagnosis is missing, which significantly hinders a possible improvement of the patient's quality of life. In this context, biosensors emerge as a future solution, opening the doors for preventive medicine and allowing the premature diagnosis of numerous pathologies. This work presents a pioneering biosensor that combines a bottom-up design approach using paper as a platform for the electrochemical recognition of peptide amyloid β-42 (Aβ-42), a biomarker for AD present in blood, associated with visible differences in the brain tissue and responsible for the formation of senile plaques. The sensor layer relies on a molecularly imprinted polymer as a biorecognition element, created on the carbon ink electrode's surface by electropolymerizing a mixture of the target analyte (Aβ-42) and a monomer (O-phenylenediamine) at neutral pH 7.2. Next, the template molecule was removed from the polymeric network by enzymatic and acidic treatments. The vacant sites so obtained preserved the shape of the imprinted protein and were able to rebind the target analyte. Morphological and chemical analyses were performed in order to control the surface modification of the materials. The analytical performance of the biosensor was evaluated by an electroanalytical technique, namely, square wave voltammetry. For this purpose, the analytical response of the biosensor was tested with standard solutions ranging from 0.1 ng/mL to 1 μg/mL of Aβ-42. The linear response of the biosensor went down to 0.1 ng/mL. Overall, the developed biosensor offered numerous benefits, such as simplicity, low cost, reproducibility, fast response, and repeatability less than 10%. All together, these features may have a strong impact in the early detection of AD.DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)UNINOVA-Instituto de Desenvolvimento de Novas TecnologiasRUNPereira, Marta V.Marques, Ana CarolinaOliveira, DanielaMartins, RodrigoMoreira, Felismina T. C.Sales, M. Goreti F.Fortunato, Elvira2021-05-05T23:27:31Z2020-06-022020-06-02T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttp://hdl.handle.net/10362/117154eng2470-1343PURE: 28385665https://doi.org/10.1021/acsomega.0c00062info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:00:13Zoai:run.unl.pt:10362/117154Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:43:30.361912Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid |
| title |
Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid |
| spellingShingle |
Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid Pereira, Marta V. Chemistry(all) Chemical Engineering(all) |
| title_short |
Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid |
| title_full |
Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid |
| title_fullStr |
Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid |
| title_full_unstemmed |
Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid |
| title_sort |
Paper-Based Platform with an in Situ Molecularly Imprinted Polymer for β-Amyloid |
| author |
Pereira, Marta V. |
| author_facet |
Pereira, Marta V. Marques, Ana Carolina Oliveira, Daniela Martins, Rodrigo Moreira, Felismina T. C. Sales, M. Goreti F. Fortunato, Elvira |
| author_role |
author |
| author2 |
Marques, Ana Carolina Oliveira, Daniela Martins, Rodrigo Moreira, Felismina T. C. Sales, M. Goreti F. Fortunato, Elvira |
| author2_role |
author author author author author author |
| dc.contributor.none.fl_str_mv |
DCM - Departamento de Ciência dos Materiais CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N) UNINOVA-Instituto de Desenvolvimento de Novas Tecnologias RUN |
| dc.contributor.author.fl_str_mv |
Pereira, Marta V. Marques, Ana Carolina Oliveira, Daniela Martins, Rodrigo Moreira, Felismina T. C. Sales, M. Goreti F. Fortunato, Elvira |
| dc.subject.por.fl_str_mv |
Chemistry(all) Chemical Engineering(all) |
| topic |
Chemistry(all) Chemical Engineering(all) |
| description |
PTDC/AAG-TEC/5400/2014 POCI-01-0145-FEDER-016637 POCI-010145-FEDER-007688 UID/CTM/50025/2019 SFRH/BD/115173/2016 SFRH/BPD/97891/2013 |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-06-02 2020-06-02T00:00:00Z 2021-05-05T23:27:31Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/117154 |
| url |
http://hdl.handle.net/10362/117154 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2470-1343 PURE: 28385665 https://doi.org/10.1021/acsomega.0c00062 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
10 application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138044601696256 |