Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells

Detalhes bibliográficos
Autor(a) principal: Gomes, Anita Q.
Data de Publicação: 2010
Outros Autores: Correia, Daniel V., Grosso, Ana R., Lança, Telma, Ferreira, Cristina, Lacerda, João F., Barata, João T., Gomes da Silva, Maria, Silva-Santos, Bruno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/6154
Resumo: ©2010 Ferrata Storti Foundation. This is an open-access paper
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spelling Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cellsBiomarkersVγ9Vδ2 T-lymphocytesHematopoietic tumorsLymphoma cell lines©2010 Ferrata Storti Foundation. This is an open-access paperBackground Vγ9Vδ2 T lymphocytes are regarded as promising mediators of cancer immunotherapy due to their capacity to eliminate multiple experimental tumors, particularly within those of hematopoietic origin. However, Vγ9Vδ2 T-cell based lymphoma clinical trials have suffered from the lack of biomarkers that can be used as prognostic of therapeutic success. Design and Methods We have conducted a comprehensive study of gene expression in acute lymphoblastic leukemias and non-Hodgkin’s lymphomas, aimed at identifying markers of susceptibility versus resistance to Vγ9Vδ2 T cell-mediated cytotoxicity. We employed cDNA microarrays and quantitative real-time PCR to screen 20 leukemia and lymphoma cell lines, and 23 primary hematopoietic tumor samples. These data were analyzed using state-of-the-art bioinformatics, and gene expression patterns were correlated with susceptibility to Vγ9Vδ2 T cell mediated cytolysis in vitro. Results We identified a panel of 10 genes encoding cell surface proteins that were statistically differentially expressed between “γδ-susceptible” and “γδ-resistant” hematopoietic tumors. Within this panel, 3 genes (ULBP1, TFR2 and IFITM1) were associated with increased susceptibility to Vγ9Vδ2 T-cell cytotoxicity, whereas the other 7 (CLEC2D, NRP2, SELL, PKD2, KCNK12, ITGA6 and SLAMF1) were enriched in resistant tumors. Furthermore, some of these candidates displayed a striking variance of expression among primary follicular lymphomas and T-cell acute lymphoblastic leukemias. Conclusions Our results suggest that hematopoietic tumors display a highly variable repertoire of surface proteins that can impact on Vγ9Vδ2 cell-mediated immunotargeting. The prognostic value of the proposed markers can now be evaluated in upcoming Vγ9Vδ2 T cell-based lymphoma/leukemia clinical trials.This work was supported by the European Molecular Biology Organization (YIP Installation Grant to BS-S),Fundação Calouste Gulbenkian (SDH Oncologia 2008 - Projecto 99293) and Fundação para a Ciência e Tecnologia/FCT (PTDC/BIA-BCM/71663/2006).Ferrata Storti FoundationRepositório da Universidade de LisboaGomes, Anita Q.Correia, Daniel V.Grosso, Ana R.Lança, TelmaFerreira, CristinaLacerda, João F.Barata, João T.Gomes da Silva, MariaSilva-Santos, Bruno2012-04-24T13:09:44Z2010-022010-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/6154engHaematologica | 2010; 95(8)0390-6078info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:48:28Zoai:repositorio.ul.pt:10451/6154Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:31:21.217193Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells
title Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells
spellingShingle Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells
Gomes, Anita Q.
Biomarkers
Vγ9Vδ2 T-lymphocytes
Hematopoietic tumors
Lymphoma cell lines
title_short Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells
title_full Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells
title_fullStr Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells
title_full_unstemmed Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells
title_sort Identification of a panel of ten cell surface protein antigens associated with immunotargeting of leukemias and lymphomas by peripheral blood γδ T cells
author Gomes, Anita Q.
author_facet Gomes, Anita Q.
Correia, Daniel V.
Grosso, Ana R.
Lança, Telma
Ferreira, Cristina
Lacerda, João F.
Barata, João T.
Gomes da Silva, Maria
Silva-Santos, Bruno
author_role author
author2 Correia, Daniel V.
Grosso, Ana R.
Lança, Telma
Ferreira, Cristina
Lacerda, João F.
Barata, João T.
Gomes da Silva, Maria
Silva-Santos, Bruno
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gomes, Anita Q.
Correia, Daniel V.
Grosso, Ana R.
Lança, Telma
Ferreira, Cristina
Lacerda, João F.
Barata, João T.
Gomes da Silva, Maria
Silva-Santos, Bruno
dc.subject.por.fl_str_mv Biomarkers
Vγ9Vδ2 T-lymphocytes
Hematopoietic tumors
Lymphoma cell lines
topic Biomarkers
Vγ9Vδ2 T-lymphocytes
Hematopoietic tumors
Lymphoma cell lines
description ©2010 Ferrata Storti Foundation. This is an open-access paper
publishDate 2010
dc.date.none.fl_str_mv 2010-02
2010-02-01T00:00:00Z
2012-04-24T13:09:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/6154
url http://hdl.handle.net/10451/6154
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Haematologica | 2010; 95(8)
0390-6078
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Ferrata Storti Foundation
publisher.none.fl_str_mv Ferrata Storti Foundation
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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