A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/746 |
Resumo: | Conditional expression of diphtheria toxin receptor (DTR) is widely used for tissue-specific ablation of cells. However, diphtheria toxin (DT) crosses the blood-brain barrier, which limits its utility for ablating peripheral cells using Cre drivers that are also expressed in the central nervous system (CNS). Here we report the development of a brain-sparing DT, termed BRAINSPAReDT, for tissue-specific genetic ablation of cells outside the CNS. We prevent blood-brain barrier passage of DT through PEGylation, which polarizes the molecule and increases its size. We validate BRAINSPAReDT with regional genetic sympathectomy: BRAINSPAReDT ablates peripheral but not central catecholaminergic neurons, thus avoiding the Parkinson-like phenotype associated with full dopaminergic depletion. Regional sympathectomy compromises adipose tissue thermogenesis, and renders mice susceptible to obesity. We provide a proof of principle that BRAINSPAReDT can be used for Cre/DTR tissue-specific ablation outside the brain using CNS drivers, while consolidating the link between adiposity and the sympathetic nervous system. |
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A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineagesChemical modificationMolecular neuroscienceNeuroimmunologyPeripheral nervous systemConditional expression of diphtheria toxin receptor (DTR) is widely used for tissue-specific ablation of cells. However, diphtheria toxin (DT) crosses the blood-brain barrier, which limits its utility for ablating peripheral cells using Cre drivers that are also expressed in the central nervous system (CNS). Here we report the development of a brain-sparing DT, termed BRAINSPAReDT, for tissue-specific genetic ablation of cells outside the CNS. We prevent blood-brain barrier passage of DT through PEGylation, which polarizes the molecule and increases its size. We validate BRAINSPAReDT with regional genetic sympathectomy: BRAINSPAReDT ablates peripheral but not central catecholaminergic neurons, thus avoiding the Parkinson-like phenotype associated with full dopaminergic depletion. Regional sympathectomy compromises adipose tissue thermogenesis, and renders mice susceptible to obesity. We provide a proof of principle that BRAINSPAReDT can be used for Cre/DTR tissue-specific ablation outside the brain using CNS drivers, while consolidating the link between adiposity and the sympathetic nervous system.Fundação para a Ciência e Tecnologia grant: (PTDC/BIM-MET/3750/2014); European Molecular Biology Organization (EMBO); European Community’s Seventh Framework Programme (FP7/2007-2013) under agreement no. (281854—the ObERStress project); MINECO co-funded by the FEDER Program of EU: (ML: SAF20015-71026-R); Xunta de Galicia grant: (ML: 2015-CP079); Instituto Gulbenkian de Ciência; Royal Society University.Nature Publishing GroupARCAPereira, Mafalda M. A.Mahú, InêsSeixas, ElsaMartinéz-Sánchez, NoeliaKubasova, NadiyaPirzgalska, Roksana MCohen, PaulDietrich, Marcelo OLópez, MiguelBernardes, Gonçalo J. L.Domingos, Ana I.2017-04-10T14:21:49Z2017-04-032017-04-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfvideo/quicktimevideo/quicktimevideo/quicktimevideo/quicktimeapplication/pdfhttp://hdl.handle.net/10400.7/746engPereira, M. M. A. et al. A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages. Nat. Commun. 8, 14967 doi: 10.1038/ncomms14967 (2017).10.1038/ncomms14967info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:08Zoai:arca.igc.gulbenkian.pt:10400.7/746Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:11:58.503026Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages |
title |
A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages |
spellingShingle |
A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages Pereira, Mafalda M. A. Chemical modification Molecular neuroscience Neuroimmunology Peripheral nervous system |
title_short |
A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages |
title_full |
A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages |
title_fullStr |
A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages |
title_full_unstemmed |
A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages |
title_sort |
A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages |
author |
Pereira, Mafalda M. A. |
author_facet |
Pereira, Mafalda M. A. Mahú, Inês Seixas, Elsa Martinéz-Sánchez, Noelia Kubasova, Nadiya Pirzgalska, Roksana M Cohen, Paul Dietrich, Marcelo O López, Miguel Bernardes, Gonçalo J. L. Domingos, Ana I. |
author_role |
author |
author2 |
Mahú, Inês Seixas, Elsa Martinéz-Sánchez, Noelia Kubasova, Nadiya Pirzgalska, Roksana M Cohen, Paul Dietrich, Marcelo O López, Miguel Bernardes, Gonçalo J. L. Domingos, Ana I. |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Pereira, Mafalda M. A. Mahú, Inês Seixas, Elsa Martinéz-Sánchez, Noelia Kubasova, Nadiya Pirzgalska, Roksana M Cohen, Paul Dietrich, Marcelo O López, Miguel Bernardes, Gonçalo J. L. Domingos, Ana I. |
dc.subject.por.fl_str_mv |
Chemical modification Molecular neuroscience Neuroimmunology Peripheral nervous system |
topic |
Chemical modification Molecular neuroscience Neuroimmunology Peripheral nervous system |
description |
Conditional expression of diphtheria toxin receptor (DTR) is widely used for tissue-specific ablation of cells. However, diphtheria toxin (DT) crosses the blood-brain barrier, which limits its utility for ablating peripheral cells using Cre drivers that are also expressed in the central nervous system (CNS). Here we report the development of a brain-sparing DT, termed BRAINSPAReDT, for tissue-specific genetic ablation of cells outside the CNS. We prevent blood-brain barrier passage of DT through PEGylation, which polarizes the molecule and increases its size. We validate BRAINSPAReDT with regional genetic sympathectomy: BRAINSPAReDT ablates peripheral but not central catecholaminergic neurons, thus avoiding the Parkinson-like phenotype associated with full dopaminergic depletion. Regional sympathectomy compromises adipose tissue thermogenesis, and renders mice susceptible to obesity. We provide a proof of principle that BRAINSPAReDT can be used for Cre/DTR tissue-specific ablation outside the brain using CNS drivers, while consolidating the link between adiposity and the sympathetic nervous system. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-04-10T14:21:49Z 2017-04-03 2017-04-03T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/746 |
url |
http://hdl.handle.net/10400.7/746 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pereira, M. M. A. et al. A brain-sparing diphtheria toxin for chemical genetic ablation of peripheral cell lineages. Nat. Commun. 8, 14967 doi: 10.1038/ncomms14967 (2017). 10.1038/ncomms14967 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf video/quicktime video/quicktime video/quicktime video/quicktime application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130574779056128 |