Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal

Detalhes bibliográficos
Autor(a) principal: Praça, Joana
Data de Publicação: 2023
Outros Autores: Furtado, Rosália, Coelho, Anabela, Correia, Cristina Belo, Borges, Vítor, Gomes, João Paulo, Pista, Angela, Batista, Rita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/8958
Resumo: (This article belongs to the Special Issue Foodborne Pathogens: Infections and Pathogenesis)
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spelling Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, PortugalListeria MonocytogenesEscherichia coliCoagulase Positive StaphylococciWhole-genome SequencingRaw Milk CheeseAlentejo PortugalGastrointestinal InfectionsFood MicrobiologySegurança AlimentarInfecções Gastrointestinais(This article belongs to the Special Issue Foodborne Pathogens: Infections and Pathogenesis)Traditional cheeses are part of the Portuguese gastronomic identity, and raw milk of autochthonous species is a common primary ingredient. Here, we investigated the presence of Listeria monocytogenes, Coagulase Positive Staphylococci (CPS) and pathogenic Escherichia coli, as well as of indicator microorganisms (E. coli and other Listeria spp.) in 96 cured raw milk cheeses from the Alentejo region. Whole genome sequencing (WGS) of pathogenic E. coli and Listeria spp. as well as antimicrobial resistance (AMR) screening of E. coli isolates was also performed. L. monocytogenes, CPS > 104 cfu/g and Extraintestinal E. coli were detected in 15.6%, 16.9% and 10.1% of the samples, respectively. Moreover, L. monocytogenes > 102 cfu/g and Staphylococcal enterotoxins were detected in 4.2% and 2.2% of the samples, respectively. AMR was observed in 27.3% of the E. coli isolates, six of which were multidrug resistant. WGS analysis unveiled clusters of high closely related isolates for both L. monocytogenes and L. innocua (often correlating with the cheese producer). This study can indicate poor hygiene practices during milk collection/preservation or during cheese-making procedures and handling, and highlights the need of more effective prevention and control measures and of multi-sectoral WGS data integration, in order to prevent and detect foodborne bacterial outbreaks.MDPIRepositório Científico do Instituto Nacional de SaúdePraça, JoanaFurtado, RosáliaCoelho, AnabelaCorreia, Cristina BeloBorges, VítorGomes, João PauloPista, AngelaBatista, Rita2024-01-24T12:01:24Z2023-01-272023-01-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/8958engMicroorganisms. 2023 Jan 27;11(2):322. doi: 10.3390/microorganisms110203222076-260710.3390/microorganisms11020322info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-27T01:31:56Zoai:repositorio.insa.pt:10400.18/8958Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:57:59.677101Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal
title Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal
spellingShingle Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal
Praça, Joana
Listeria Monocytogenes
Escherichia coli
Coagulase Positive Staphylococci
Whole-genome Sequencing
Raw Milk Cheese
Alentejo Portugal
Gastrointestinal Infections
Food Microbiology
Segurança Alimentar
Infecções Gastrointestinais
title_short Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal
title_full Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal
title_fullStr Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal
title_full_unstemmed Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal
title_sort Listeria monocytogenes, Escherichia coli and Coagulase Positive Staphylococci in Cured Raw Milk Cheese from Alentejo Region, Portugal
author Praça, Joana
author_facet Praça, Joana
Furtado, Rosália
Coelho, Anabela
Correia, Cristina Belo
Borges, Vítor
Gomes, João Paulo
Pista, Angela
Batista, Rita
author_role author
author2 Furtado, Rosália
Coelho, Anabela
Correia, Cristina Belo
Borges, Vítor
Gomes, João Paulo
Pista, Angela
Batista, Rita
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Praça, Joana
Furtado, Rosália
Coelho, Anabela
Correia, Cristina Belo
Borges, Vítor
Gomes, João Paulo
Pista, Angela
Batista, Rita
dc.subject.por.fl_str_mv Listeria Monocytogenes
Escherichia coli
Coagulase Positive Staphylococci
Whole-genome Sequencing
Raw Milk Cheese
Alentejo Portugal
Gastrointestinal Infections
Food Microbiology
Segurança Alimentar
Infecções Gastrointestinais
topic Listeria Monocytogenes
Escherichia coli
Coagulase Positive Staphylococci
Whole-genome Sequencing
Raw Milk Cheese
Alentejo Portugal
Gastrointestinal Infections
Food Microbiology
Segurança Alimentar
Infecções Gastrointestinais
description (This article belongs to the Special Issue Foodborne Pathogens: Infections and Pathogenesis)
publishDate 2023
dc.date.none.fl_str_mv 2023-01-27
2023-01-27T00:00:00Z
2024-01-24T12:01:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/8958
url http://hdl.handle.net/10400.18/8958
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms. 2023 Jan 27;11(2):322. doi: 10.3390/microorganisms11020322
2076-2607
10.3390/microorganisms11020322
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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