Human adipose tissue primary cultures and impact in prostate cancer

• Autor(a) principal: Cunha, Virgínia Filipa Pereira Monteiro da
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10773/8985
• topic: Biologia molecular; Cancro da próstata; Tecidos adiposos; Patologia celular

Prostate cancer (Pca) is one of the most frequent diagnosed neoplasies and the second cause of cancer-related death in the world, in men. Between others risk factors, obesity has been associated to Pca although the innerent mechanisms to this association remain to be clear. With this work, throuhg in vitro studies, we wish to contribute to the understanding of the impact of white adipose tissue and its sub-fractions (adipocytes and stromal vascular fraction), from visceral and periprostatic anatomic regions, in celular proliferation, apoptosis and invasion of castration sensitivity (LNCaP) and castration resistant (PC-3) prostate cells. With the purpose of obtaining answers directly from humam studies, were performed visceral and periprostatic adipose tissue primary cultures obtained during urologic surgeries (radical prostatectomy and prostatic adenomectomy) (n=16). Adipose tissue was used to make primary organotipical cultures (WAT) and after collagenase digestion adipocytes and SVF primary cultures. Sobrenatants and infranatants of each culture were collect and used as conditioned medium representing adipokines production. LNCaP and PC-3 cell lines were stimulated with these mediums and apoptosis, proliferation and invasion were evaluated, in vitro. This study model represent a potential form for analyze the impact of adipose tissue in tumor cells, allowing to evaluate adipose tissue-tumor interactions. The results show that adipose tissue promotes tumor cells proliferation, that periprostatic adipose tissue increase apoptosis in obese individuals and that SVF subfraction suppresses invasion of PC-3 cells through a direct effect in tumor cells.