Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10198/23412 |
Resumo: | Echinacea purpurea (L.) Moench is widely known for its medicinal properties, being one of the most used medicinal plants for its immunostimulant properties. Nevertheless, there is still scarce information on its cytotoxic activity. Thus, this study aims at evaluating the cytotoxicity and antimicrobial activity of several aqueous and organic extracts of the aerial parts of this plant and chemically characterizing the obtained extracts. The analysis was performed by HPLC–DAD–ESI/MS. Fifteen compounds were identified; of these, seven were phenolic acids and eight were flavonoids. Non-polar compounds were evaluated by GC/MS, with a total of sixty-four compounds identified, and the most abundant groups were the sterols, fatty acids and long-chain hydrocarbons. The highest antimicrobial activity was exhibited by the dichloromethane, ethyl acetate, and acetone extracts. Dichloromethane and n-hexane extracts showed the highest cytotoxic activity. Therefore, they were fractionated, and the obtained fractions were also assessed for their cytotoxicity. Notwithstanding, the cytotoxicity of the extracts was superior to that of the obtained fractions, evidencing a possible synergistic effect of different compounds in the whole extracts. |
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Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractionsAntimicrobial activityCytotoxicityEchinacea purpurea (L.) MoenchPhenolic compoundsPolar compoundsEchinacea purpurea (L.) Moench is widely known for its medicinal properties, being one of the most used medicinal plants for its immunostimulant properties. Nevertheless, there is still scarce information on its cytotoxic activity. Thus, this study aims at evaluating the cytotoxicity and antimicrobial activity of several aqueous and organic extracts of the aerial parts of this plant and chemically characterizing the obtained extracts. The analysis was performed by HPLC–DAD–ESI/MS. Fifteen compounds were identified; of these, seven were phenolic acids and eight were flavonoids. Non-polar compounds were evaluated by GC/MS, with a total of sixty-four compounds identified, and the most abundant groups were the sterols, fatty acids and long-chain hydrocarbons. The highest antimicrobial activity was exhibited by the dichloromethane, ethyl acetate, and acetone extracts. Dichloromethane and n-hexane extracts showed the highest cytotoxic activity. Therefore, they were fractionated, and the obtained fractions were also assessed for their cytotoxicity. Notwithstanding, the cytotoxicity of the extracts was superior to that of the obtained fractions, evidencing a possible synergistic effect of different compounds in the whole extracts.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020) and CQ-VR (UID/QUI/00616/2013); national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros and R. C. Calhelha contracts. FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.Biblioteca Digital do IPBCoelho, Joana C.M.M.Barros, LillianDias, Maria InêsFinimundy, Tiane C.Amaral, Joana S.Alves, Maria JoséCalhelha, Ricardo C.Santos, Paulo F.Ferreira, Isabel C.F.R.2018-01-19T10:00:00Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/23412engCoelho, Joana; Barros, Lillian; Dias, Maria Inês; Finimundy, Tiane C.; Amaral, Joana S.; Alves, Maria José; Calhelha, Ricardo C.; Santos, P. F.; Ferreira, Isabel C.F.R. (2020). Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions. Pharmaceuticals. ISSN 1424-8247. 13:6, p. 1-1610.3390/ph13060125info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:52:21Zoai:bibliotecadigital.ipb.pt:10198/23412Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:14:24.745607Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions |
title |
Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions |
spellingShingle |
Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions Coelho, Joana C.M.M. Antimicrobial activity Cytotoxicity Echinacea purpurea (L.) Moench Phenolic compounds Polar compounds |
title_short |
Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions |
title_full |
Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions |
title_fullStr |
Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions |
title_full_unstemmed |
Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions |
title_sort |
Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions |
author |
Coelho, Joana C.M.M. |
author_facet |
Coelho, Joana C.M.M. Barros, Lillian Dias, Maria Inês Finimundy, Tiane C. Amaral, Joana S. Alves, Maria José Calhelha, Ricardo C. Santos, Paulo F. Ferreira, Isabel C.F.R. |
author_role |
author |
author2 |
Barros, Lillian Dias, Maria Inês Finimundy, Tiane C. Amaral, Joana S. Alves, Maria José Calhelha, Ricardo C. Santos, Paulo F. Ferreira, Isabel C.F.R. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Biblioteca Digital do IPB |
dc.contributor.author.fl_str_mv |
Coelho, Joana C.M.M. Barros, Lillian Dias, Maria Inês Finimundy, Tiane C. Amaral, Joana S. Alves, Maria José Calhelha, Ricardo C. Santos, Paulo F. Ferreira, Isabel C.F.R. |
dc.subject.por.fl_str_mv |
Antimicrobial activity Cytotoxicity Echinacea purpurea (L.) Moench Phenolic compounds Polar compounds |
topic |
Antimicrobial activity Cytotoxicity Echinacea purpurea (L.) Moench Phenolic compounds Polar compounds |
description |
Echinacea purpurea (L.) Moench is widely known for its medicinal properties, being one of the most used medicinal plants for its immunostimulant properties. Nevertheless, there is still scarce information on its cytotoxic activity. Thus, this study aims at evaluating the cytotoxicity and antimicrobial activity of several aqueous and organic extracts of the aerial parts of this plant and chemically characterizing the obtained extracts. The analysis was performed by HPLC–DAD–ESI/MS. Fifteen compounds were identified; of these, seven were phenolic acids and eight were flavonoids. Non-polar compounds were evaluated by GC/MS, with a total of sixty-four compounds identified, and the most abundant groups were the sterols, fatty acids and long-chain hydrocarbons. The highest antimicrobial activity was exhibited by the dichloromethane, ethyl acetate, and acetone extracts. Dichloromethane and n-hexane extracts showed the highest cytotoxic activity. Therefore, they were fractionated, and the obtained fractions were also assessed for their cytotoxicity. Notwithstanding, the cytotoxicity of the extracts was superior to that of the obtained fractions, evidencing a possible synergistic effect of different compounds in the whole extracts. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-01-19T10:00:00Z 2020 2020-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10198/23412 |
url |
http://hdl.handle.net/10198/23412 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Coelho, Joana; Barros, Lillian; Dias, Maria Inês; Finimundy, Tiane C.; Amaral, Joana S.; Alves, Maria José; Calhelha, Ricardo C.; Santos, P. F.; Ferreira, Isabel C.F.R. (2020). Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions. Pharmaceuticals. ISSN 1424-8247. 13:6, p. 1-16 10.3390/ph13060125 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135423339954176 |