Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions

Detalhes bibliográficos
Autor(a) principal: Coelho, Joana C.M.M.
Data de Publicação: 2018
Outros Autores: Barros, Lillian, Dias, Maria Inês, Finimundy, Tiane C., Amaral, Joana S., Alves, Maria José, Calhelha, Ricardo C., Santos, Paulo F., Ferreira, Isabel C.F.R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/23412
Resumo: Echinacea purpurea (L.) Moench is widely known for its medicinal properties, being one of the most used medicinal plants for its immunostimulant properties. Nevertheless, there is still scarce information on its cytotoxic activity. Thus, this study aims at evaluating the cytotoxicity and antimicrobial activity of several aqueous and organic extracts of the aerial parts of this plant and chemically characterizing the obtained extracts. The analysis was performed by HPLC–DAD–ESI/MS. Fifteen compounds were identified; of these, seven were phenolic acids and eight were flavonoids. Non-polar compounds were evaluated by GC/MS, with a total of sixty-four compounds identified, and the most abundant groups were the sterols, fatty acids and long-chain hydrocarbons. The highest antimicrobial activity was exhibited by the dichloromethane, ethyl acetate, and acetone extracts. Dichloromethane and n-hexane extracts showed the highest cytotoxic activity. Therefore, they were fractionated, and the obtained fractions were also assessed for their cytotoxicity. Notwithstanding, the cytotoxicity of the extracts was superior to that of the obtained fractions, evidencing a possible synergistic effect of different compounds in the whole extracts.
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spelling Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractionsAntimicrobial activityCytotoxicityEchinacea purpurea (L.) MoenchPhenolic compoundsPolar compoundsEchinacea purpurea (L.) Moench is widely known for its medicinal properties, being one of the most used medicinal plants for its immunostimulant properties. Nevertheless, there is still scarce information on its cytotoxic activity. Thus, this study aims at evaluating the cytotoxicity and antimicrobial activity of several aqueous and organic extracts of the aerial parts of this plant and chemically characterizing the obtained extracts. The analysis was performed by HPLC–DAD–ESI/MS. Fifteen compounds were identified; of these, seven were phenolic acids and eight were flavonoids. Non-polar compounds were evaluated by GC/MS, with a total of sixty-four compounds identified, and the most abundant groups were the sterols, fatty acids and long-chain hydrocarbons. The highest antimicrobial activity was exhibited by the dichloromethane, ethyl acetate, and acetone extracts. Dichloromethane and n-hexane extracts showed the highest cytotoxic activity. Therefore, they were fractionated, and the obtained fractions were also assessed for their cytotoxicity. Notwithstanding, the cytotoxicity of the extracts was superior to that of the obtained fractions, evidencing a possible synergistic effect of different compounds in the whole extracts.The authors are grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020) and CQ-VR (UID/QUI/00616/2013); national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros and R. C. Calhelha contracts. FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E.Biblioteca Digital do IPBCoelho, Joana C.M.M.Barros, LillianDias, Maria InêsFinimundy, Tiane C.Amaral, Joana S.Alves, Maria JoséCalhelha, Ricardo C.Santos, Paulo F.Ferreira, Isabel C.F.R.2018-01-19T10:00:00Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/23412engCoelho, Joana; Barros, Lillian; Dias, Maria Inês; Finimundy, Tiane C.; Amaral, Joana S.; Alves, Maria José; Calhelha, Ricardo C.; Santos, P. F.; Ferreira, Isabel C.F.R. (2020). Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions. Pharmaceuticals. ISSN 1424-8247. 13:6, p. 1-1610.3390/ph13060125info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:52:21Zoai:bibliotecadigital.ipb.pt:10198/23412Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:14:24.745607Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
title Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
spellingShingle Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
Coelho, Joana C.M.M.
Antimicrobial activity
Cytotoxicity
Echinacea purpurea (L.) Moench
Phenolic compounds
Polar compounds
title_short Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
title_full Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
title_fullStr Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
title_full_unstemmed Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
title_sort Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions
author Coelho, Joana C.M.M.
author_facet Coelho, Joana C.M.M.
Barros, Lillian
Dias, Maria Inês
Finimundy, Tiane C.
Amaral, Joana S.
Alves, Maria José
Calhelha, Ricardo C.
Santos, Paulo F.
Ferreira, Isabel C.F.R.
author_role author
author2 Barros, Lillian
Dias, Maria Inês
Finimundy, Tiane C.
Amaral, Joana S.
Alves, Maria José
Calhelha, Ricardo C.
Santos, Paulo F.
Ferreira, Isabel C.F.R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Coelho, Joana C.M.M.
Barros, Lillian
Dias, Maria Inês
Finimundy, Tiane C.
Amaral, Joana S.
Alves, Maria José
Calhelha, Ricardo C.
Santos, Paulo F.
Ferreira, Isabel C.F.R.
dc.subject.por.fl_str_mv Antimicrobial activity
Cytotoxicity
Echinacea purpurea (L.) Moench
Phenolic compounds
Polar compounds
topic Antimicrobial activity
Cytotoxicity
Echinacea purpurea (L.) Moench
Phenolic compounds
Polar compounds
description Echinacea purpurea (L.) Moench is widely known for its medicinal properties, being one of the most used medicinal plants for its immunostimulant properties. Nevertheless, there is still scarce information on its cytotoxic activity. Thus, this study aims at evaluating the cytotoxicity and antimicrobial activity of several aqueous and organic extracts of the aerial parts of this plant and chemically characterizing the obtained extracts. The analysis was performed by HPLC–DAD–ESI/MS. Fifteen compounds were identified; of these, seven were phenolic acids and eight were flavonoids. Non-polar compounds were evaluated by GC/MS, with a total of sixty-four compounds identified, and the most abundant groups were the sterols, fatty acids and long-chain hydrocarbons. The highest antimicrobial activity was exhibited by the dichloromethane, ethyl acetate, and acetone extracts. Dichloromethane and n-hexane extracts showed the highest cytotoxic activity. Therefore, they were fractionated, and the obtained fractions were also assessed for their cytotoxicity. Notwithstanding, the cytotoxicity of the extracts was superior to that of the obtained fractions, evidencing a possible synergistic effect of different compounds in the whole extracts.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-19T10:00:00Z
2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/23412
url http://hdl.handle.net/10198/23412
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Coelho, Joana; Barros, Lillian; Dias, Maria Inês; Finimundy, Tiane C.; Amaral, Joana S.; Alves, Maria José; Calhelha, Ricardo C.; Santos, P. F.; Ferreira, Isabel C.F.R. (2020). Echinacea purpurea (L.) moench: chemical characterization and bioactivity of its extracts and fractions. Pharmaceuticals. ISSN 1424-8247. 13:6, p. 1-16
10.3390/ph13060125
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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