A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease

Detalhes bibliográficos
Autor(a) principal: Lemos, M. A.
Data de Publicação: 2000
Outros Autores: Teixeira, J. A., Mota, M., Gama, F. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/1183
Resumo: Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.
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spelling A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a proteaseCapillary electrophoresisCore-binding domainTrichoderma reeseiUltrafiltrationScience & TechnologyCore-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI.KluwerUniversidade do MinhoLemos, M. A.Teixeira, J. A.Mota, M.Gama, F. M.20002000-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/1183eng“Biotechnology letters”. ISSN 0141-5492. 22 (2000) 703–707.0141-549210.1023/A:1005691821173info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:17:15Zoai:repositorium.sdum.uminho.pt:1822/1183Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:09:50.685361Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
title A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
spellingShingle A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
Lemos, M. A.
Capillary electrophoresis
Core-binding domain
Trichoderma reesei
Ultrafiltration
Science & Technology
title_short A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
title_full A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
title_fullStr A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
title_full_unstemmed A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
title_sort A simple method to separate cellulose-binding domains of fungal cellulases after digestion by a protease
author Lemos, M. A.
author_facet Lemos, M. A.
Teixeira, J. A.
Mota, M.
Gama, F. M.
author_role author
author2 Teixeira, J. A.
Mota, M.
Gama, F. M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lemos, M. A.
Teixeira, J. A.
Mota, M.
Gama, F. M.
dc.subject.por.fl_str_mv Capillary electrophoresis
Core-binding domain
Trichoderma reesei
Ultrafiltration
Science & Technology
topic Capillary electrophoresis
Core-binding domain
Trichoderma reesei
Ultrafiltration
Science & Technology
description Core-binding domains of fungal cellulases from Trichoderma reesei were purified using a new and simple technique. Cellulases were hydrolysed with papain and the binding domains were then separated from the digested mixture by ultrafiltration. The enzymatic digestion process was monitored using capillary electrophoresis. This methodology produced a yield of 85% of binding domains.
publishDate 2000
dc.date.none.fl_str_mv 2000
2000-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/1183
url http://hdl.handle.net/1822/1183
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv “Biotechnology letters”. ISSN 0141-5492. 22 (2000) 703–707.
0141-5492
10.1023/A:1005691821173
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Kluwer
publisher.none.fl_str_mv Kluwer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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