Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia

Detalhes bibliográficos
Autor(a) principal: Teixeira, Cristiana Alexandra Ferreira Monteiro
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/1431
Resumo: Tese de mestrado, Biologia (Biologia Humana e Ambiente), 2009, Universidade de Lisboa, Faculdade de Ciências
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spelling Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemiaBiologia celularLeucemiaLinfocitoseTeses de mestradoTese de mestrado, Biologia (Biologia Humana e Ambiente), 2009, Universidade de Lisboa, Faculdade de CiênciasDuring tumor angiogenesis bone marrow (BM)-derived endothelial progenitor cells (EPC) are recruited to circulation and contribute to the neo-vasculature of growing tumors. Although the pathophysiologic role of angiogenesis in solid tumors has been extensively studied, its involvement in hematological malignancies has only recently been recognized. Increased angiogenesis has been documented in patients with Chronic Lymphocytic Leukemia (CLL). The World Health Organization classification of hematopoietic neoplasias recognizes some lymphomas and corresponding lymphoid leukemias as different phases (solid and circulating, respectively) of the same disease: CLL as a leukemic, lymphocytic lymphoma, only distinguishable from small lymphocytic lymphoma (SLL) by its leukemic presentation and lower aggressiveness. It arises mainly from a slow accumulation of mature-looking malignant B lymphocyte population resistant to apoptosis, in the peripheral blood (PB), BM and/or lymph nodes (LN). Given the clinical importance of distinguishing leukemic from lymphoma forms of CLL, we characterized the angiogenic and vasculogenic profile of CLL patients to test the hypothesis that there might be differences in angiogenesis-related parameters in circulation (circulating endothelial progenitor cells (CEPC) and vascular endothelial growth factor (VEGF) and incorporated into the affected BM and LN tissues between the two phases of the disease. Our data shows that patients with SLL under treatment have higher number of circulating EPC and higher levels of circulating VEGF. Importantly, we show that CEPC are functional in CLL and SLL patients, as seen in vitro in endothelial differentiation assays. We also observed increased microvessel density, decreased tumor cell apoptosis and higher CD38 expression by the tumor cells in BM biopsies from SLL patients which could be related with increased aggressiveness and bad prognosis of the disease at this phase. The miRNAs expression profile showed that there are different miRNAs patterns associated with SLL and CLL phases. Our study demonstrates for the first time significant differences in angiogenic and vasculogenic factors in circulation and in the affected tissues between leukemia and lymphoma phases, as suggested by a significant higher number of CEPC in SLL patients vs. CLL patients; this result together with increased MVD observed in BM of SLL patients could be related with a more aggressive phenotype.Resumo alargado em português disponível no documentoDias, Sérgio Jerónimo RodriguesRodrigues, Maria GabrielaRepositório da Universidade de LisboaTeixeira, Cristiana Alexandra Ferreira Monteiro2010-07-27T08:58:15Z20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1431enghttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000570142info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:40:56Zoai:repositorio.ul.pt:10451/1431Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:28:04.543473Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
title Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
spellingShingle Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
Teixeira, Cristiana Alexandra Ferreira Monteiro
Biologia celular
Leucemia
Linfocitose
Teses de mestrado
title_short Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
title_full Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
title_fullStr Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
title_full_unstemmed Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
title_sort Angiogenic and vasculogenic phenotyping allows distinguishing lymphoma from leukemic 'stages' of chronic lymphocytic leukemia
author Teixeira, Cristiana Alexandra Ferreira Monteiro
author_facet Teixeira, Cristiana Alexandra Ferreira Monteiro
author_role author
dc.contributor.none.fl_str_mv Dias, Sérgio Jerónimo Rodrigues
Rodrigues, Maria Gabriela
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Teixeira, Cristiana Alexandra Ferreira Monteiro
dc.subject.por.fl_str_mv Biologia celular
Leucemia
Linfocitose
Teses de mestrado
topic Biologia celular
Leucemia
Linfocitose
Teses de mestrado
description Tese de mestrado, Biologia (Biologia Humana e Ambiente), 2009, Universidade de Lisboa, Faculdade de Ciências
publishDate 2009
dc.date.none.fl_str_mv 2009
2009-01-01T00:00:00Z
2010-07-27T08:58:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1431
url http://hdl.handle.net/10451/1431
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000570142
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
text/xml
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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