Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal

Detalhes bibliográficos
Autor(a) principal: Rocha, Rafael
Data de Publicação: 2023
Outros Autores: Gonçalves, Luzia, Conceição, Cláudia, Andrade, Patrícia, Cristóvão, José Manuel, Condeço, Jorge, Delgado, Beatriz, Caeiro, Cristina, Kuzmenko, Tetyana, Vasconcelos, Eugénia, Escoval, Maria Antónia, Rey, Carmen, Guz, Madalina, Norte, Cláudia, Aldeia, Carlos, Cruz, Diego, Maia, Carla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/163181
Resumo: Funding Information: This paper has been sponsored by Elanco Animal Health in the framework of the CVBD®World Forum Symposium. The authors would like to acknowledge all the blood donors who agreed to participate in this study for contributing with their answers to the questionnaire and their blood samples. The authors would also like to acknowledge all the professionals of the following institutions that contributed to organization of physical equipment, presentation of the study to potential participants, obtaining of informed consent, separation and storage of samples and questionnaires: Instituto Português do Sangue e da Transplantação; Centro Hospitalar Universitário do Algarve; Hospital do Espírito Santo de Évora; Unidade Local de Sáude do Norte Alentejano; Unidade Local de Saúde do Baixo Alentejo and Unidade Local de Saúde do Litoral Alentejano. Lastly, the authors would like to acknowledge the researcher Regina Loesch for her contribution to filling in the electronic database with answers to the paper questionnaires, and the researcher Ana Rita Goes for her contributions to designing the questionnaire for this study. Funding Information: RR was supported by the Portuguese Ministry of Education and Science (via Fundação para a Ciência e a Tecnologia, I.P.—FCT) through a PhD grant (UI/BD/151067/2021). The authors would also like to acknowledge FCT for funding through contracts with GHTM (UID/Multi/04413/2020, UIDB/00006/2020 and UIDP/00006/2020) and LA-REAL (LA/P/0117/2020). This project was also funded by the Tropical Clinic Unit (Institute of Hygiene and Tropical Medicine [IHMT]). Publisher Copyright: © 2023, BioMed Central Ltd., part of Springer Nature.
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spelling Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland PortugalAsymptomaticBlood donorKnowledgeLeishmaniaLeishmaniasisOne HealthPerceptionsPortugalPracticesSeroprevalenceR MedicineRA0421 Public health. Hygiene. Preventive MedicineParasitologyveterinary(all)Infectious DiseasesSDG 3 - Good Health and Well-beingSDG 13 - Climate ActionFunding Information: This paper has been sponsored by Elanco Animal Health in the framework of the CVBD®World Forum Symposium. The authors would like to acknowledge all the blood donors who agreed to participate in this study for contributing with their answers to the questionnaire and their blood samples. The authors would also like to acknowledge all the professionals of the following institutions that contributed to organization of physical equipment, presentation of the study to potential participants, obtaining of informed consent, separation and storage of samples and questionnaires: Instituto Português do Sangue e da Transplantação; Centro Hospitalar Universitário do Algarve; Hospital do Espírito Santo de Évora; Unidade Local de Sáude do Norte Alentejano; Unidade Local de Saúde do Baixo Alentejo and Unidade Local de Saúde do Litoral Alentejano. Lastly, the authors would like to acknowledge the researcher Regina Loesch for her contribution to filling in the electronic database with answers to the paper questionnaires, and the researcher Ana Rita Goes for her contributions to designing the questionnaire for this study. Funding Information: RR was supported by the Portuguese Ministry of Education and Science (via Fundação para a Ciência e a Tecnologia, I.P.—FCT) through a PhD grant (UI/BD/151067/2021). The authors would also like to acknowledge FCT for funding through contracts with GHTM (UID/Multi/04413/2020, UIDB/00006/2020 and UIDP/00006/2020) and LA-REAL (LA/P/0117/2020). This project was also funded by the Tropical Clinic Unit (Institute of Hygiene and Tropical Medicine [IHMT]). Publisher Copyright: © 2023, BioMed Central Ltd., part of Springer Nature.Background: Asymptomatic infection is the most common outcome of exposure to Leishmania parasites. In the Mediterranean region, where Leishmania infantum is endemic, studies on the prevalence of asymptomatic infection have often relied on serological testing in blood donors. In Spain, regional studies have shown seroprevalence in blood donors between 1 and 8%; in Portugal, values of 0 and 2% were suggested by two localized studies, in different populations. The purpose of this study was (i) to estimate the prevalence of asymptomatic Leishmania infection in blood donors in mainland Portugal, and (ii) to study the association between the detection of antibodies to Leishmania and sociodemographic factors, and also the knowledge, perceptions and practices (KPP) of the blood donors regarding leishmaniasis. Methods: A cross-sectional study targeted the population of people who donated blood in mainland Portugal. Participants, distributed proportionally by municipality and aged between 18 and 65 years, were selected randomly in 347 blood collection points between February and June 2022, and completed a sociodemographic and a KPP questionnaire. Detection of anti-Leishmania antibodies in serum was performed using an ELISA commercial kit. Individual KPP scores were calculated by adding grades defined for each question. Results: Globally, 201/3763 samples were positive. The estimated national true seroprevalence was 4.8% (95% CI 4.1–5.5%). The proportion of positive results was significantly different between NUTS (Nomenclature of Territorial Units for Statistics) regions. Models suggested that seropositivity was significantly higher in male sex, people older than 25 years, or residing in the Centro NUTS2 region, but not in dog owners nor people with lower KPP scores. Overall, 72.3% of participants had previously heard of leishmaniasis and, in multivariate analysis, a higher Knowledge score was associated with age 25–40 years, female sex, ownership of dogs, and higher education. Conclusions: Global estimated true seroprevalence (4.8%) was similar to previous regional studies in blood donors in neighboring Spain. Higher seroprevalence values in the NUTS2 Centro region were consistent with incidence data from humans and seroprevalence studies in dogs. On the other hand, the low values in the Alentejo and the high values in the northern subregions may be the result of geographical shifts in parasite circulation due to climate change and should prompt localized and integrated, vector, canine, and human research, following a One Health approach. Graphical Abstract: [Figure not available: see fulltext.].Population health, policies and services (PPS)Global Health and Tropical Medicine (GHTM)Instituto de Higiene e Medicina Tropical (IHMT)Individual Health Care (IHC)Vector borne diseases and pathogens (VBD)RUNRocha, RafaelGonçalves, LuziaConceição, CláudiaAndrade, PatríciaCristóvão, José ManuelCondeço, JorgeDelgado, BeatrizCaeiro, CristinaKuzmenko, TetyanaVasconcelos, EugéniaEscoval, Maria AntóniaRey, CarmenGuz, MadalinaNorte, CláudiaAldeia, CarlosCruz, DiegoMaia, Carla2024-02-06T23:19:57Z2023-122023-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article22application/pdfhttp://hdl.handle.net/10362/163181eng1756-3305PURE: 75196904https://doi.org/10.1186/s13071-023-05980-1info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:46:16Zoai:run.unl.pt:10362/163181Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:59:17.547387Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal
title Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal
spellingShingle Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal
Rocha, Rafael
Asymptomatic
Blood donor
Knowledge
Leishmania
Leishmaniasis
One Health
Perceptions
Portugal
Practices
Seroprevalence
R Medicine
RA0421 Public health. Hygiene. Preventive Medicine
Parasitology
veterinary(all)
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
title_short Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal
title_full Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal
title_fullStr Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal
title_full_unstemmed Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal
title_sort Prevalence of asymptomatic Leishmania infection and knowledge, perceptions, and practices in blood donors in mainland Portugal
author Rocha, Rafael
author_facet Rocha, Rafael
Gonçalves, Luzia
Conceição, Cláudia
Andrade, Patrícia
Cristóvão, José Manuel
Condeço, Jorge
Delgado, Beatriz
Caeiro, Cristina
Kuzmenko, Tetyana
Vasconcelos, Eugénia
Escoval, Maria Antónia
Rey, Carmen
Guz, Madalina
Norte, Cláudia
Aldeia, Carlos
Cruz, Diego
Maia, Carla
author_role author
author2 Gonçalves, Luzia
Conceição, Cláudia
Andrade, Patrícia
Cristóvão, José Manuel
Condeço, Jorge
Delgado, Beatriz
Caeiro, Cristina
Kuzmenko, Tetyana
Vasconcelos, Eugénia
Escoval, Maria Antónia
Rey, Carmen
Guz, Madalina
Norte, Cláudia
Aldeia, Carlos
Cruz, Diego
Maia, Carla
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Population health, policies and services (PPS)
Global Health and Tropical Medicine (GHTM)
Instituto de Higiene e Medicina Tropical (IHMT)
Individual Health Care (IHC)
Vector borne diseases and pathogens (VBD)
RUN
dc.contributor.author.fl_str_mv Rocha, Rafael
Gonçalves, Luzia
Conceição, Cláudia
Andrade, Patrícia
Cristóvão, José Manuel
Condeço, Jorge
Delgado, Beatriz
Caeiro, Cristina
Kuzmenko, Tetyana
Vasconcelos, Eugénia
Escoval, Maria Antónia
Rey, Carmen
Guz, Madalina
Norte, Cláudia
Aldeia, Carlos
Cruz, Diego
Maia, Carla
dc.subject.por.fl_str_mv Asymptomatic
Blood donor
Knowledge
Leishmania
Leishmaniasis
One Health
Perceptions
Portugal
Practices
Seroprevalence
R Medicine
RA0421 Public health. Hygiene. Preventive Medicine
Parasitology
veterinary(all)
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
topic Asymptomatic
Blood donor
Knowledge
Leishmania
Leishmaniasis
One Health
Perceptions
Portugal
Practices
Seroprevalence
R Medicine
RA0421 Public health. Hygiene. Preventive Medicine
Parasitology
veterinary(all)
Infectious Diseases
SDG 3 - Good Health and Well-being
SDG 13 - Climate Action
description Funding Information: This paper has been sponsored by Elanco Animal Health in the framework of the CVBD®World Forum Symposium. The authors would like to acknowledge all the blood donors who agreed to participate in this study for contributing with their answers to the questionnaire and their blood samples. The authors would also like to acknowledge all the professionals of the following institutions that contributed to organization of physical equipment, presentation of the study to potential participants, obtaining of informed consent, separation and storage of samples and questionnaires: Instituto Português do Sangue e da Transplantação; Centro Hospitalar Universitário do Algarve; Hospital do Espírito Santo de Évora; Unidade Local de Sáude do Norte Alentejano; Unidade Local de Saúde do Baixo Alentejo and Unidade Local de Saúde do Litoral Alentejano. Lastly, the authors would like to acknowledge the researcher Regina Loesch for her contribution to filling in the electronic database with answers to the paper questionnaires, and the researcher Ana Rita Goes for her contributions to designing the questionnaire for this study. Funding Information: RR was supported by the Portuguese Ministry of Education and Science (via Fundação para a Ciência e a Tecnologia, I.P.—FCT) through a PhD grant (UI/BD/151067/2021). The authors would also like to acknowledge FCT for funding through contracts with GHTM (UID/Multi/04413/2020, UIDB/00006/2020 and UIDP/00006/2020) and LA-REAL (LA/P/0117/2020). This project was also funded by the Tropical Clinic Unit (Institute of Hygiene and Tropical Medicine [IHMT]). Publisher Copyright: © 2023, BioMed Central Ltd., part of Springer Nature.
publishDate 2023
dc.date.none.fl_str_mv 2023-12
2023-12-01T00:00:00Z
2024-02-06T23:19:57Z
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PURE: 75196904
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