An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca

Detalhes bibliográficos
Autor(a) principal: Mesquita, Elsa
Data de Publicação: 2021
Outros Autores: Ribeiro, Rita, Silva, Carla J. C., Alves, Rita, Baptista, Rita, Condinho, Sílvia, Rosa, Maria João, Perdigão, João, Caneiras, Catia, Duarte, Aida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/47498
Resumo: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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spelling An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytocaWastewater treatment plant (WWTP)CTX-M-15SED-1ESBLKPC-3 carbapenemaseKlebsiella oxytocaOchromobactrum intermediumCitrobacter farmeriPortugal© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Wastewater treatment plants (WWTPs) are significant reservoirs of bacterial resistance. This work aims to identify the determinants of resistance produced by Gram-negative bacteria in the influent and effluent of two WWTPs in Portugal. A total of 96 wastewater samples were obtained between 2016 and 2019. The numbers of total aerobic and fecal contamination bacteria were evaluated, and genomic features were searched by polymerase chain reaction (PCR) and Next-Generation Sequencing (NGS). Enterobacteriaceae corresponded to 78.6% (n = 161) of the 205 isolates identified by 16sRNA. The most frequent isolates were Escherichia spp. (57.1%, n = 117), followed by Aeromonas spp. (16.1%, n = 33) and Klebsiella spp. (12.7%, n = 26). The remaining 29 isolates (14.1%) were distributed across 10 different genera. Among the 183 resistant genes detected, 54 isolates produced extended spectrum β-lactamases (ESBL), of which blaCTX-M-15 was predominant (37 isolates; 68.5%). A KPC-3 carbapenemase-producing K. oxytoca was identified (n = 1), with blaKPC-3 included in a transposon Tn4401 isoform b. A higher number of virulence genes (VG) (19 genes) was found in the E. coli 5301 (O25b-ST131-B2) isolate compared with a commensal E. coli 5281 (O25b-ST410-A) (six genes). Both shared five VG [Enterobactin; Aerobactin, CFA/1 (clade α); Type1 (clade γ1); Type IV]. In conclusion, this work highlights the role of relevant clinical bacteria in WWTPs, such as KPC-3-producing K. oxytoca, and, for the first time, a CTX-M-15-producing Ochromobactrum intermedium, a human opportunistic pathogen, and a SED-1-producing Citrobacter farmeri, an uncommon CTX-M-type extended-spectrum beta-lactamase.This work was supported by the European Union LIFE Programme under Grant Agreement LIFE14 ENV/PT/000739—LIFE Impetus (https://life-impetus.eu/), accessed on 1 February 2021.MDPIRepositório da Universidade de LisboaMesquita, ElsaRibeiro, RitaSilva, Carla J. C.Alves, RitaBaptista, RitaCondinho, SílviaRosa, Maria JoãoPerdigão, JoãoCaneiras, CatiaDuarte, Aida2021-04-21T12:01:05Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/47498engMicroorganisms. 2021 Mar 11;9(3):57610.3390/microorganisms90305762076-2607info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:50:28Zoai:repositorio.ul.pt:10451/47498Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:59:31.587195Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca
title An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca
spellingShingle An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca
Mesquita, Elsa
Wastewater treatment plant (WWTP)
CTX-M-15
SED-1
ESBL
KPC-3 carbapenemase
Klebsiella oxytoca
Ochromobactrum intermedium
Citrobacter farmeri
Portugal
title_short An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca
title_full An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca
title_fullStr An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca
title_full_unstemmed An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca
title_sort An update on wastewater multi-resistant bacteria : identification of clinical pathogens such as Escherichia coli O25b:H4-B2-ST131-producing CTX-M-15 ESBL and KPC-3 Carbapenemase-Producing Klebsiella oxytoca
author Mesquita, Elsa
author_facet Mesquita, Elsa
Ribeiro, Rita
Silva, Carla J. C.
Alves, Rita
Baptista, Rita
Condinho, Sílvia
Rosa, Maria João
Perdigão, João
Caneiras, Catia
Duarte, Aida
author_role author
author2 Ribeiro, Rita
Silva, Carla J. C.
Alves, Rita
Baptista, Rita
Condinho, Sílvia
Rosa, Maria João
Perdigão, João
Caneiras, Catia
Duarte, Aida
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Mesquita, Elsa
Ribeiro, Rita
Silva, Carla J. C.
Alves, Rita
Baptista, Rita
Condinho, Sílvia
Rosa, Maria João
Perdigão, João
Caneiras, Catia
Duarte, Aida
dc.subject.por.fl_str_mv Wastewater treatment plant (WWTP)
CTX-M-15
SED-1
ESBL
KPC-3 carbapenemase
Klebsiella oxytoca
Ochromobactrum intermedium
Citrobacter farmeri
Portugal
topic Wastewater treatment plant (WWTP)
CTX-M-15
SED-1
ESBL
KPC-3 carbapenemase
Klebsiella oxytoca
Ochromobactrum intermedium
Citrobacter farmeri
Portugal
description © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
publishDate 2021
dc.date.none.fl_str_mv 2021-04-21T12:01:05Z
2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/47498
url http://hdl.handle.net/10451/47498
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Microorganisms. 2021 Mar 11;9(3):576
10.3390/microorganisms9030576
2076-2607
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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